Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS22240

Experiment: R2A with Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS22235 and RR42_RS22240 are separated by 821 nucleotidesRR42_RS22240 and RR42_RS22245 are separated by 364 nucleotides RR42_RS22235: RR42_RS22235 - peptidase M19, at 326,256 to 326,885 _RS22235 RR42_RS22240: RR42_RS22240 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 327,707 to 329,008 _RS22240 RR42_RS22245: RR42_RS22245 - cytochrome C biogenesis protein, at 329,373 to 330,320 _RS22245 Position (kb) 327 328 329 330Strain fitness (log2 ratio) -2 -1 0 1 2at 326.827 kb on + strandat 326.828 kb on - strandat 326.828 kb on - strandat 327.005 kb on - strandat 327.651 kb on + strandat 327.652 kb on - strandat 327.652 kb on - strandat 327.697 kb on - strandat 327.751 kb on + strandat 327.752 kb on - strandat 327.752 kb on - strandat 327.752 kb on - strandat 327.755 kb on - strandat 327.902 kb on - strand, within RR42_RS22240at 327.902 kb on - strand, within RR42_RS22240at 327.902 kb on - strand, within RR42_RS22240at 328.196 kb on + strand, within RR42_RS22240at 328.196 kb on + strand, within RR42_RS22240at 328.197 kb on - strand, within RR42_RS22240at 328.197 kb on - strand, within RR42_RS22240at 328.724 kb on + strand, within RR42_RS22240at 328.850 kb on + strand, within RR42_RS22240at 329.007 kb on - strandat 329.024 kb on + strandat 329.176 kb on + strandat 329.177 kb on - strandat 329.177 kb on - strandat 329.177 kb on - strandat 329.177 kb on - strandat 329.197 kb on - strandat 329.228 kb on + strandat 329.228 kb on + strandat 329.295 kb on + strandat 329.295 kb on + strandat 329.296 kb on - strandat 329.297 kb on + strandat 329.297 kb on + strandat 329.297 kb on + strandat 329.297 kb on + strandat 329.298 kb on - strandat 329.298 kb on - strandat 329.301 kb on + strandat 329.302 kb on - strandat 329.302 kb on - strandat 329.302 kb on - strandat 329.379 kb on + strandat 329.380 kb on - strandat 329.391 kb on + strandat 329.392 kb on - strandat 329.466 kb on + strandat 329.467 kb on - strandat 329.467 kb on - strandat 329.529 kb on + strand, within RR42_RS22245at 329.530 kb on - strand, within RR42_RS22245at 329.587 kb on - strand, within RR42_RS22245at 329.640 kb on + strand, within RR42_RS22245at 329.640 kb on + strand, within RR42_RS22245at 329.834 kb on + strand, within RR42_RS22245at 329.834 kb on + strand, within RR42_RS22245at 329.983 kb on - strand, within RR42_RS22245

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM
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326,827 + +1.1
326,828 - +0.6
326,828 - +0.4
327,005 - +0.1
327,651 + -0.5
327,652 - +0.7
327,652 - -0.1
327,697 - +0.6
327,751 + +2.7
327,752 - -0.6
327,752 - -0.6
327,752 - +0.7
327,755 - -0.7
327,902 - RR42_RS22240 0.15 +0.5
327,902 - RR42_RS22240 0.15 +0.6
327,902 - RR42_RS22240 0.15 -0.5
328,196 + RR42_RS22240 0.38 -0.1
328,196 + RR42_RS22240 0.38 +0.6
328,197 - RR42_RS22240 0.38 +1.1
328,197 - RR42_RS22240 0.38 -1.5
328,724 + RR42_RS22240 0.78 +0.7
328,850 + RR42_RS22240 0.88 +0.3
329,007 - +2.4
329,024 + +0.5
329,176 + +0.1
329,177 - -0.5
329,177 - +0.7
329,177 - +0.2
329,177 - +1.3
329,197 - +0.1
329,228 + -0.2
329,228 + +0.6
329,295 + +0.8
329,295 + -0.1
329,296 - +0.6
329,297 + +1.1
329,297 + -0.0
329,297 + +0.9
329,297 + +0.4
329,298 - +0.4
329,298 - +0.2
329,301 + +0.6
329,302 - -0.3
329,302 - -0.0
329,302 - -0.3
329,379 + +0.7
329,380 - -0.0
329,391 + -0.2
329,392 - -0.2
329,466 + +0.5
329,467 - +0.7
329,467 - -0.3
329,529 + RR42_RS22245 0.16 -0.7
329,530 - RR42_RS22245 0.17 +0.2
329,587 - RR42_RS22245 0.23 -1.2
329,640 + RR42_RS22245 0.28 -0.7
329,640 + RR42_RS22245 0.28 -1.8
329,834 + RR42_RS22245 0.49 +0.7
329,834 + RR42_RS22245 0.49 -1.4
329,983 - RR42_RS22245 0.64 -0.5

Or see this region's nucleotide sequence