Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22180 and RR42_RS22185 are separated by 159 nucleotides RR42_RS22185 and RR42_RS22190 are separated by 123 nucleotides
RR42_RS22180: RR42_RS22180 - hypothetical protein, at 313,760 to 314,458
_RS22180
RR42_RS22185: RR42_RS22185 - LysR family transcriptional regulator, at 314,618 to 315,583
_RS22185
RR42_RS22190: RR42_RS22190 - ABC transporter substrate-binding protein, at 315,707 to 316,678
_RS22190
Position (kb) 314
315
316 Strain fitness (log2 ratio) -1
0
1 at 313.703 kb on + strand at 313.703 kb on + strand at 313.704 kb on - strand at 313.704 kb on - strand at 313.921 kb on + strand, within RR42_RS22180 at 314.363 kb on - strand, within RR42_RS22180 at 314.465 kb on + strand at 314.472 kb on - strand at 314.550 kb on + strand at 314.550 kb on + strand at 314.550 kb on + strand at 314.550 kb on + strand at 314.550 kb on + strand at 314.550 kb on + strand at 314.551 kb on - strand at 314.551 kb on - strand at 314.551 kb on - strand at 314.551 kb on - strand at 314.551 kb on - strand at 314.576 kb on + strand at 314.576 kb on + strand at 314.577 kb on - strand at 314.693 kb on - strand at 314.764 kb on - strand, within RR42_RS22185 at 314.764 kb on - strand, within RR42_RS22185 at 314.764 kb on - strand, within RR42_RS22185 at 315.152 kb on + strand, within RR42_RS22185 at 315.364 kb on + strand, within RR42_RS22185 at 315.783 kb on - strand at 315.783 kb on - strand at 315.783 kb on - strand at 315.815 kb on + strand, within RR42_RS22190 at 315.965 kb on + strand, within RR42_RS22190 at 315.965 kb on + strand, within RR42_RS22190 at 315.966 kb on - strand, within RR42_RS22190 at 316.080 kb on + strand, within RR42_RS22190 at 316.163 kb on + strand, within RR42_RS22190 at 316.164 kb on - strand, within RR42_RS22190 at 316.164 kb on - strand, within RR42_RS22190 at 316.164 kb on - strand, within RR42_RS22190 at 316.195 kb on + strand, within RR42_RS22190 at 316.196 kb on - strand, within RR42_RS22190 at 316.196 kb on - strand, within RR42_RS22190 at 316.250 kb on + strand, within RR42_RS22190 at 316.251 kb on - strand, within RR42_RS22190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 313,703 + -0.4 313,703 + -1.0 313,704 - -0.4 313,704 - -0.4 313,921 + RR42_RS22180 0.23 -0.4 314,363 - RR42_RS22180 0.86 +0.2 314,465 + -0.3 314,472 - -0.8 314,550 + -1.0 314,550 + -0.4 314,550 + +0.9 314,550 + -0.9 314,550 + +0.3 314,550 + +1.0 314,551 - +1.0 314,551 - -0.4 314,551 - +0.2 314,551 - -0.3 314,551 - +0.1 314,576 + +1.2 314,576 + -0.1 314,577 - +0.4 314,693 - -0.0 314,764 - RR42_RS22185 0.15 +0.2 314,764 - RR42_RS22185 0.15 +0.2 314,764 - RR42_RS22185 0.15 +0.3 315,152 + RR42_RS22185 0.55 +1.1 315,364 + RR42_RS22185 0.77 +1.0 315,783 - +0.9 315,783 - +0.9 315,783 - -0.2 315,815 + RR42_RS22190 0.11 -0.2 315,965 + RR42_RS22190 0.27 -0.5 315,965 + RR42_RS22190 0.27 +0.6 315,966 - RR42_RS22190 0.27 -0.2 316,080 + RR42_RS22190 0.38 +0.1 316,163 + RR42_RS22190 0.47 -0.2 316,164 - RR42_RS22190 0.47 +1.7 316,164 - RR42_RS22190 0.47 -0.7 316,164 - RR42_RS22190 0.47 -0.6 316,195 + RR42_RS22190 0.50 -1.0 316,196 - RR42_RS22190 0.50 -1.6 316,196 - RR42_RS22190 0.50 +0.5 316,250 + RR42_RS22190 0.56 +0.1 316,251 - RR42_RS22190 0.56 -0.4
Or see this region's nucleotide sequence