Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21045 and RR42_RS21050 are separated by 125 nucleotides RR42_RS21050 and RR42_RS21055 are separated by 123 nucleotides RR42_RS21055 and RR42_RS21060 are separated by 408 nucleotides
RR42_RS21045: RR42_RS21045 - cyanate hydratase, at 47,320 to 47,763
_RS21045
RR42_RS21050: RR42_RS21050 - hypothetical protein, at 47,889 to 48,107
_RS21050
RR42_RS21055: RR42_RS21055 - excisionase, at 48,231 to 48,836
_RS21055
RR42_RS21060: RR42_RS21060 - histidine kinase, at 49,245 to 51,272
_RS21060
Position (kb) 48
49 Strain fitness (log2 ratio) -1
0
1
2
3 at 47.335 kb on - strand at 47.533 kb on + strand, within RR42_RS21045 at 47.533 kb on + strand, within RR42_RS21045 at 47.534 kb on - strand, within RR42_RS21045 at 47.534 kb on - strand, within RR42_RS21045 at 47.587 kb on + strand, within RR42_RS21045 at 47.587 kb on + strand, within RR42_RS21045 at 47.587 kb on + strand, within RR42_RS21045 at 47.587 kb on + strand, within RR42_RS21045 at 47.587 kb on + strand, within RR42_RS21045 at 47.588 kb on - strand, within RR42_RS21045 at 47.588 kb on - strand, within RR42_RS21045 at 47.588 kb on - strand, within RR42_RS21045 at 47.606 kb on - strand, within RR42_RS21045 at 47.606 kb on - strand, within RR42_RS21045 at 47.606 kb on - strand, within RR42_RS21045 at 47.749 kb on + strand at 47.758 kb on + strand at 47.863 kb on - strand at 48.042 kb on + strand, within RR42_RS21050 at 48.043 kb on - strand, within RR42_RS21050 at 48.071 kb on + strand, within RR42_RS21050 at 48.072 kb on - strand, within RR42_RS21050 at 48.072 kb on - strand, within RR42_RS21050 at 48.140 kb on + strand at 48.184 kb on + strand at 48.185 kb on - strand at 48.232 kb on + strand at 48.317 kb on + strand, within RR42_RS21055 at 48.318 kb on - strand, within RR42_RS21055 at 48.509 kb on - strand, within RR42_RS21055 at 48.607 kb on - strand, within RR42_RS21055 at 48.836 kb on + strand at 48.836 kb on + strand at 48.836 kb on + strand at 48.836 kb on + strand at 48.837 kb on - strand at 48.837 kb on - strand at 48.837 kb on - strand at 48.837 kb on - strand at 48.837 kb on - strand at 48.837 kb on - strand at 48.889 kb on - strand at 48.889 kb on - strand at 48.894 kb on - strand at 48.894 kb on - strand at 48.905 kb on + strand at 48.987 kb on + strand at 49.067 kb on + strand at 49.068 kb on - strand at 49.068 kb on - strand at 49.074 kb on + strand at 49.075 kb on - strand at 49.075 kb on - strand at 49.075 kb on - strand at 49.157 kb on + strand at 49.157 kb on + strand at 49.157 kb on + strand at 49.157 kb on + strand at 49.158 kb on - strand at 49.158 kb on - strand at 49.158 kb on - strand at 49.192 kb on + strand at 49.192 kb on + strand at 49.193 kb on - strand at 49.193 kb on - strand at 49.194 kb on + strand at 49.194 kb on + strand at 49.195 kb on - strand at 49.195 kb on - strand at 49.208 kb on + strand at 49.233 kb on + strand at 49.337 kb on - strand at 49.418 kb on + strand at 49.418 kb on + strand at 49.515 kb on + strand, within RR42_RS21060 at 49.516 kb on - strand, within RR42_RS21060 at 49.662 kb on + strand, within RR42_RS21060 at 49.663 kb on - strand, within RR42_RS21060 at 49.800 kb on + strand, within RR42_RS21060 at 49.801 kb on - strand, within RR42_RS21060 at 49.801 kb on - strand, within RR42_RS21060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 47,335 - +0.4 47,533 + RR42_RS21045 0.48 +0.8 47,533 + RR42_RS21045 0.48 -0.7 47,534 - RR42_RS21045 0.48 +0.6 47,534 - RR42_RS21045 0.48 -0.6 47,587 + RR42_RS21045 0.60 +1.0 47,587 + RR42_RS21045 0.60 +0.1 47,587 + RR42_RS21045 0.60 +0.9 47,587 + RR42_RS21045 0.60 +1.8 47,587 + RR42_RS21045 0.60 -0.6 47,588 - RR42_RS21045 0.60 +0.4 47,588 - RR42_RS21045 0.60 +0.7 47,588 - RR42_RS21045 0.60 +0.4 47,606 - RR42_RS21045 0.64 -0.0 47,606 - RR42_RS21045 0.64 -0.5 47,606 - RR42_RS21045 0.64 +3.3 47,749 + +0.9 47,758 + +0.1 47,863 - -0.7 48,042 + RR42_RS21050 0.70 -0.2 48,043 - RR42_RS21050 0.70 -0.2 48,071 + RR42_RS21050 0.83 +0.5 48,072 - RR42_RS21050 0.84 -0.9 48,072 - RR42_RS21050 0.84 +0.1 48,140 + -0.1 48,184 + +0.4 48,185 - -0.1 48,232 + -0.5 48,317 + RR42_RS21055 0.14 +0.1 48,318 - RR42_RS21055 0.14 +0.9 48,509 - RR42_RS21055 0.46 +0.4 48,607 - RR42_RS21055 0.62 +0.5 48,836 + -0.3 48,836 + -0.2 48,836 + +0.3 48,836 + -0.4 48,837 - -1.3 48,837 - +1.9 48,837 - -0.9 48,837 - -1.3 48,837 - -0.2 48,837 - +1.0 48,889 - -1.1 48,889 - -0.6 48,894 - +0.8 48,894 - +0.9 48,905 + -1.5 48,987 + -1.6 49,067 + -0.0 49,068 - +0.1 49,068 - +0.4 49,074 + -0.8 49,075 - -1.2 49,075 - -0.7 49,075 - -0.6 49,157 + -0.5 49,157 + -0.3 49,157 + +0.9 49,157 + -1.3 49,158 - -1.3 49,158 - -0.7 49,158 - +0.5 49,192 + +0.4 49,192 + +0.2 49,193 - -1.1 49,193 - +0.6 49,194 + +0.3 49,194 + +0.1 49,195 - +0.8 49,195 - +1.0 49,208 + +0.2 49,233 + -1.2 49,337 - +0.2 49,418 + +0.7 49,418 + -0.1 49,515 + RR42_RS21060 0.13 -0.7 49,516 - RR42_RS21060 0.13 +0.2 49,662 + RR42_RS21060 0.21 +0.5 49,663 - RR42_RS21060 0.21 -0.4 49,800 + RR42_RS21060 0.27 -1.7 49,801 - RR42_RS21060 0.27 -0.5 49,801 - RR42_RS21060 0.27 -0.0
Or see this region's nucleotide sequence