Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20275 and RR42_RS20280 are separated by 95 nucleotides RR42_RS20280 and RR42_RS20285 are separated by 9 nucleotides RR42_RS20285 and RR42_RS20290 are separated by 381 nucleotides
RR42_RS20275: RR42_RS20275 - ABC transporter permease, at 4,385,888 to 4,387,096
_RS20275
RR42_RS20280: RR42_RS20280 - histidinol dehydrogenase, at 4,387,192 to 4,387,917
_RS20280
RR42_RS20285: RR42_RS20285 - amino acid ABC transporter ATP-binding protein, at 4,387,927 to 4,388,703
_RS20285
RR42_RS20290: RR42_RS20290 - choline dehydrogenase, at 4,389,085 to 4,390,785
_RS20290
Position (kb)
4387
4388
4389 Strain fitness (log2 ratio)
-1
0
1 at 4386.969 kb on + strand, within RR42_RS20275 at 4386.993 kb on + strand at 4386.994 kb on - strand at 4387.109 kb on + strand at 4387.110 kb on - strand at 4387.146 kb on + strand at 4387.147 kb on - strand at 4387.284 kb on - strand, within RR42_RS20280 at 4387.284 kb on - strand, within RR42_RS20280 at 4387.344 kb on - strand, within RR42_RS20280 at 4387.545 kb on - strand, within RR42_RS20280 at 4387.545 kb on - strand, within RR42_RS20280 at 4387.665 kb on - strand, within RR42_RS20280 at 4387.965 kb on + strand at 4387.966 kb on - strand at 4387.966 kb on - strand at 4387.967 kb on + strand at 4387.967 kb on + strand at 4387.967 kb on + strand at 4387.967 kb on + strand at 4387.968 kb on - strand at 4387.996 kb on + strand at 4387.997 kb on - strand at 4388.007 kb on - strand, within RR42_RS20285 at 4388.042 kb on - strand, within RR42_RS20285 at 4388.360 kb on + strand, within RR42_RS20285 at 4388.361 kb on - strand, within RR42_RS20285 at 4388.526 kb on + strand, within RR42_RS20285 at 4388.526 kb on + strand, within RR42_RS20285 at 4388.527 kb on - strand, within RR42_RS20285 at 4388.852 kb on + strand at 4388.852 kb on + strand at 4388.853 kb on - strand at 4388.856 kb on + strand at 4388.856 kb on + strand at 4388.857 kb on - strand at 4388.857 kb on - strand at 4388.869 kb on + strand at 4388.870 kb on - strand at 4388.972 kb on + strand at 4388.972 kb on + strand at 4388.972 kb on + strand at 4388.972 kb on + strand at 4388.973 kb on - strand at 4389.082 kb on + strand at 4389.082 kb on + strand at 4389.082 kb on + strand at 4389.558 kb on - strand, within RR42_RS20290 at 4389.558 kb on - strand, within RR42_RS20290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 4,386,969 + RR42_RS20275 0.89 -0.8 4,386,993 + +0.1 4,386,994 - -0.5 4,387,109 + +1.3 4,387,110 - -0.5 4,387,146 + +0.3 4,387,147 - -0.3 4,387,284 - RR42_RS20280 0.13 +0.6 4,387,284 - RR42_RS20280 0.13 -0.0 4,387,344 - RR42_RS20280 0.21 -0.1 4,387,545 - RR42_RS20280 0.49 -0.4 4,387,545 - RR42_RS20280 0.49 +0.3 4,387,665 - RR42_RS20280 0.65 -1.0 4,387,965 + +0.2 4,387,966 - -0.1 4,387,966 - -0.9 4,387,967 + +0.4 4,387,967 + +0.9 4,387,967 + +0.2 4,387,967 + +0.4 4,387,968 - +0.0 4,387,996 + -0.7 4,387,997 - +1.1 4,388,007 - RR42_RS20285 0.10 +0.1 4,388,042 - RR42_RS20285 0.15 -1.1 4,388,360 + RR42_RS20285 0.56 +1.3 4,388,361 - RR42_RS20285 0.56 +1.3 4,388,526 + RR42_RS20285 0.77 +1.7 4,388,526 + RR42_RS20285 0.77 +0.7 4,388,527 - RR42_RS20285 0.77 -0.6 4,388,852 + +0.0 4,388,852 + +0.1 4,388,853 - -0.1 4,388,856 + -0.1 4,388,856 + -0.1 4,388,857 - -0.2 4,388,857 - -0.1 4,388,869 + +0.2 4,388,870 - +0.8 4,388,972 + -1.1 4,388,972 + +0.1 4,388,972 + +0.6 4,388,972 + +1.0 4,388,973 - +0.1 4,389,082 + +0.3 4,389,082 + -0.1 4,389,082 + -0.2 4,389,558 - RR42_RS20290 0.28 +0.6 4,389,558 - RR42_RS20290 0.28 -0.1
Or see this region's nucleotide sequence