Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19550 and RR42_RS19555 are separated by 138 nucleotides RR42_RS19555 and RR42_RS19560 are separated by 59 nucleotides RR42_RS19560 and RR42_RS19565 overlap by 4 nucleotides
RR42_RS19550: RR42_RS19550 - hypothetical protein, at 4,226,496 to 4,227,194
_RS19550
RR42_RS19555: RR42_RS19555 - hypothetical protein, at 4,227,333 to 4,227,704
_RS19555
RR42_RS19560: RR42_RS19560 - hypothetical protein, at 4,227,764 to 4,228,375
_RS19560
RR42_RS19565: RR42_RS19565 - sugar nucleotide epimerase YfcH family protein, at 4,228,372 to 4,229,883
_RS19565
Position (kb)
4227
4228 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4226.433 kb on + strand at 4226.433 kb on + strand at 4226.433 kb on + strand at 4226.433 kb on + strand at 4226.434 kb on - strand at 4226.434 kb on - strand at 4226.434 kb on - strand at 4226.434 kb on - strand at 4226.576 kb on - strand, within RR42_RS19550 at 4226.576 kb on - strand, within RR42_RS19550 at 4226.631 kb on + strand, within RR42_RS19550 at 4226.631 kb on + strand, within RR42_RS19550 at 4226.737 kb on + strand, within RR42_RS19550 at 4226.738 kb on - strand, within RR42_RS19550 at 4226.738 kb on - strand, within RR42_RS19550 at 4226.738 kb on - strand, within RR42_RS19550 at 4226.895 kb on - strand, within RR42_RS19550 at 4226.941 kb on - strand, within RR42_RS19550 at 4226.962 kb on + strand, within RR42_RS19550 at 4226.962 kb on + strand, within RR42_RS19550 at 4226.963 kb on - strand, within RR42_RS19550 at 4227.020 kb on + strand, within RR42_RS19550 at 4227.021 kb on - strand, within RR42_RS19550 at 4227.021 kb on - strand, within RR42_RS19550 at 4227.115 kb on + strand, within RR42_RS19550 at 4227.115 kb on + strand, within RR42_RS19550 at 4227.209 kb on + strand at 4227.211 kb on + strand at 4227.237 kb on + strand at 4227.237 kb on + strand at 4227.237 kb on + strand at 4227.238 kb on - strand at 4227.257 kb on - strand at 4227.308 kb on + strand at 4227.332 kb on - strand at 4227.332 kb on - strand at 4227.661 kb on + strand, within RR42_RS19555 at 4227.662 kb on - strand, within RR42_RS19555 at 4227.671 kb on + strand at 4227.672 kb on - strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.752 kb on + strand at 4227.753 kb on - strand at 4227.753 kb on - strand at 4227.753 kb on - strand at 4227.783 kb on + strand at 4227.784 kb on - strand at 4227.784 kb on - strand at 4227.843 kb on + strand, within RR42_RS19560 at 4227.843 kb on + strand, within RR42_RS19560 at 4227.844 kb on - strand, within RR42_RS19560 at 4227.855 kb on + strand, within RR42_RS19560 at 4227.856 kb on - strand, within RR42_RS19560 at 4227.895 kb on - strand, within RR42_RS19560 at 4228.326 kb on + strand at 4228.326 kb on + strand at 4228.364 kb on + strand at 4228.443 kb on - strand at 4228.443 kb on - strand at 4228.624 kb on - strand, within RR42_RS19565 at 4228.624 kb on - strand, within RR42_RS19565 at 4228.625 kb on + strand, within RR42_RS19565 at 4228.625 kb on + strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 4,226,433 + +0.6 4,226,433 + +0.1 4,226,433 + -0.4 4,226,433 + -0.1 4,226,434 - +1.4 4,226,434 - -1.1 4,226,434 - +1.0 4,226,434 - +0.7 4,226,576 - RR42_RS19550 0.11 -0.0 4,226,576 - RR42_RS19550 0.11 +1.0 4,226,631 + RR42_RS19550 0.19 +1.7 4,226,631 + RR42_RS19550 0.19 -1.7 4,226,737 + RR42_RS19550 0.34 -0.9 4,226,738 - RR42_RS19550 0.35 +0.3 4,226,738 - RR42_RS19550 0.35 -1.3 4,226,738 - RR42_RS19550 0.35 -2.1 4,226,895 - RR42_RS19550 0.57 -0.3 4,226,941 - RR42_RS19550 0.64 -0.1 4,226,962 + RR42_RS19550 0.67 -0.1 4,226,962 + RR42_RS19550 0.67 -0.4 4,226,963 - RR42_RS19550 0.67 +0.7 4,227,020 + RR42_RS19550 0.75 -0.0 4,227,021 - RR42_RS19550 0.75 -0.9 4,227,021 - RR42_RS19550 0.75 +0.7 4,227,115 + RR42_RS19550 0.89 -1.3 4,227,115 + RR42_RS19550 0.89 -0.2 4,227,209 + +1.4 4,227,211 + -1.4 4,227,237 + -0.1 4,227,237 + +0.6 4,227,237 + -0.8 4,227,238 - +1.1 4,227,257 - -1.0 4,227,308 + +0.2 4,227,332 - -1.1 4,227,332 - -1.5 4,227,661 + RR42_RS19555 0.88 +0.9 4,227,662 - RR42_RS19555 0.88 +0.1 4,227,671 + -0.2 4,227,672 - -0.7 4,227,673 + -1.4 4,227,673 + +0.9 4,227,673 + +1.0 4,227,673 + +0.1 4,227,673 + -0.8 4,227,674 - +0.2 4,227,674 - +0.8 4,227,674 - +0.5 4,227,674 - -0.8 4,227,674 - -0.3 4,227,752 + -0.0 4,227,753 - +0.0 4,227,753 - -1.8 4,227,753 - -0.7 4,227,783 + -2.8 4,227,784 - +0.2 4,227,784 - +0.1 4,227,843 + RR42_RS19560 0.13 -0.6 4,227,843 + RR42_RS19560 0.13 +0.4 4,227,844 - RR42_RS19560 0.13 +0.3 4,227,855 + RR42_RS19560 0.15 +0.3 4,227,856 - RR42_RS19560 0.15 +0.5 4,227,895 - RR42_RS19560 0.21 -1.0 4,228,326 + -0.4 4,228,326 + +0.6 4,228,364 + +0.2 4,228,443 - +0.5 4,228,443 - -1.0 4,228,624 - RR42_RS19565 0.17 +1.1 4,228,624 - RR42_RS19565 0.17 +1.4 4,228,625 + RR42_RS19565 0.17 -0.3 4,228,625 + RR42_RS19565 0.17 -2.4 4,228,626 - RR42_RS19565 0.17 +0.4 4,228,626 - RR42_RS19565 0.17 -1.3 4,228,626 - RR42_RS19565 0.17 +0.0 4,228,626 - RR42_RS19565 0.17 -0.1
Or see this region's nucleotide sequence