Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18000 and RR42_RS18005 are separated by 4 nucleotides RR42_RS18005 and RR42_RS18010 are separated by 39 nucleotides RR42_RS18010 and RR42_RS18015 overlap by 4 nucleotides
RR42_RS18000: RR42_RS18000 - cell division protein ZapD, at 3,893,931 to 3,894,689
_RS18000
RR42_RS18005: RR42_RS18005 - pilus assembly protein, at 3,894,694 to 3,894,879
_RS18005
RR42_RS18010: RR42_RS18010 - 7,8-dihydro-8-oxoguanine-triphosphatase, at 3,894,919 to 3,895,359
_RS18010
RR42_RS18015: RR42_RS18015 - ATPase AAA, at 3,895,356 to 3,896,258
_RS18015
Position (kb)
3894
3895
3896 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3893.923 kb on + strand at 3893.923 kb on + strand at 3893.923 kb on + strand at 3893.924 kb on - strand at 3893.924 kb on - strand at 3893.940 kb on + strand at 3893.946 kb on + strand at 3893.947 kb on - strand at 3893.954 kb on + strand at 3893.955 kb on - strand at 3894.009 kb on + strand, within RR42_RS18000 at 3894.010 kb on - strand, within RR42_RS18000 at 3894.342 kb on + strand, within RR42_RS18000 at 3894.342 kb on + strand, within RR42_RS18000 at 3894.342 kb on + strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.495 kb on + strand, within RR42_RS18000 at 3894.495 kb on + strand, within RR42_RS18000 at 3894.495 kb on + strand, within RR42_RS18000 at 3894.496 kb on - strand, within RR42_RS18000 at 3894.522 kb on + strand, within RR42_RS18000 at 3894.522 kb on + strand, within RR42_RS18000 at 3894.523 kb on - strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.704 kb on + strand at 3894.704 kb on + strand at 3894.704 kb on + strand at 3894.753 kb on + strand, within RR42_RS18005 at 3894.754 kb on - strand, within RR42_RS18005 at 3894.754 kb on - strand, within RR42_RS18005 at 3894.820 kb on + strand, within RR42_RS18005 at 3894.821 kb on - strand, within RR42_RS18005 at 3894.821 kb on - strand, within RR42_RS18005 at 3894.877 kb on + strand at 3894.877 kb on + strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.966 kb on + strand, within RR42_RS18010 at 3894.966 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.083 kb on - strand, within RR42_RS18010 at 3895.083 kb on - strand, within RR42_RS18010 at 3895.083 kb on - strand, within RR42_RS18010 at 3895.095 kb on - strand, within RR42_RS18010 at 3895.095 kb on - strand, within RR42_RS18010 at 3895.232 kb on + strand, within RR42_RS18010 at 3895.232 kb on + strand, within RR42_RS18010 at 3895.233 kb on - strand, within RR42_RS18010 at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.552 kb on + strand, within RR42_RS18015 at 3895.552 kb on + strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.573 kb on + strand, within RR42_RS18015 at 3895.666 kb on + strand, within RR42_RS18015 at 3895.667 kb on - strand, within RR42_RS18015 at 3895.687 kb on + strand, within RR42_RS18015 at 3895.723 kb on + strand, within RR42_RS18015 at 3895.723 kb on + strand, within RR42_RS18015 at 3895.723 kb on + strand, within RR42_RS18015 at 3895.724 kb on - strand, within RR42_RS18015 at 3896.030 kb on + strand, within RR42_RS18015 at 3896.031 kb on - strand, within RR42_RS18015 at 3896.031 kb on - strand, within RR42_RS18015 at 3896.116 kb on + strand, within RR42_RS18015 at 3896.117 kb on - strand, within RR42_RS18015 at 3896.231 kb on + strand at 3896.231 kb on + strand at 3896.231 kb on + strand at 3896.231 kb on + strand at 3896.232 kb on - strand at 3896.232 kb on - strand at 3896.232 kb on - strand at 3896.232 kb on - strand at 3896.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,893,923 + +0.5 3,893,923 + -0.2 3,893,923 + +0.0 3,893,924 - -0.7 3,893,924 - -1.7 3,893,940 + +0.6 3,893,946 + -0.3 3,893,947 - +0.1 3,893,954 + -0.8 3,893,955 - -1.2 3,894,009 + RR42_RS18000 0.10 +0.4 3,894,010 - RR42_RS18000 0.10 -0.3 3,894,342 + RR42_RS18000 0.54 -0.2 3,894,342 + RR42_RS18000 0.54 -1.0 3,894,342 + RR42_RS18000 0.54 +1.0 3,894,343 - RR42_RS18000 0.54 +0.5 3,894,343 - RR42_RS18000 0.54 +0.8 3,894,343 - RR42_RS18000 0.54 +0.0 3,894,343 - RR42_RS18000 0.54 +1.8 3,894,495 + RR42_RS18000 0.74 -0.0 3,894,495 + RR42_RS18000 0.74 -0.8 3,894,495 + RR42_RS18000 0.74 +0.0 3,894,496 - RR42_RS18000 0.74 -2.4 3,894,522 + RR42_RS18000 0.78 -0.6 3,894,522 + RR42_RS18000 0.78 +0.9 3,894,523 - RR42_RS18000 0.78 -1.0 3,894,591 + RR42_RS18000 0.87 -0.8 3,894,591 + RR42_RS18000 0.87 +0.8 3,894,591 + RR42_RS18000 0.87 +0.0 3,894,591 + RR42_RS18000 0.87 -0.4 3,894,591 + RR42_RS18000 0.87 +0.3 3,894,591 + RR42_RS18000 0.87 -1.7 3,894,592 - RR42_RS18000 0.87 -0.8 3,894,592 - RR42_RS18000 0.87 -0.8 3,894,592 - RR42_RS18000 0.87 -0.1 3,894,592 - RR42_RS18000 0.87 -1.3 3,894,592 - RR42_RS18000 0.87 +0.0 3,894,704 + +1.5 3,894,704 + -0.1 3,894,704 + +0.1 3,894,753 + RR42_RS18005 0.32 -0.0 3,894,754 - RR42_RS18005 0.32 -0.6 3,894,754 - RR42_RS18005 0.32 -0.7 3,894,820 + RR42_RS18005 0.68 +0.8 3,894,821 - RR42_RS18005 0.68 -1.4 3,894,821 - RR42_RS18005 0.68 +0.1 3,894,877 + +0.0 3,894,877 + +0.1 3,894,878 - -1.1 3,894,878 - +0.0 3,894,878 - -1.4 3,894,878 - +1.3 3,894,878 - +1.1 3,894,878 - -1.7 3,894,966 + RR42_RS18010 0.11 +0.5 3,894,966 + RR42_RS18010 0.11 -0.0 3,894,985 + RR42_RS18010 0.15 -0.6 3,894,985 + RR42_RS18010 0.15 +0.7 3,894,985 + RR42_RS18010 0.15 -0.2 3,894,985 + RR42_RS18010 0.15 -0.2 3,894,986 - RR42_RS18010 0.15 +0.9 3,894,986 - RR42_RS18010 0.15 -0.2 3,894,986 - RR42_RS18010 0.15 +0.7 3,894,986 - RR42_RS18010 0.15 +0.2 3,894,986 - RR42_RS18010 0.15 -0.1 3,894,986 - RR42_RS18010 0.15 +0.7 3,894,986 - RR42_RS18010 0.15 -1.0 3,895,082 + RR42_RS18010 0.37 -1.5 3,895,082 + RR42_RS18010 0.37 -0.5 3,895,082 + RR42_RS18010 0.37 -0.1 3,895,082 + RR42_RS18010 0.37 -0.7 3,895,082 + RR42_RS18010 0.37 -0.5 3,895,082 + RR42_RS18010 0.37 -1.7 3,895,082 + RR42_RS18010 0.37 +0.2 3,895,082 + RR42_RS18010 0.37 -1.2 3,895,082 + RR42_RS18010 0.37 +0.9 3,895,082 + RR42_RS18010 0.37 +0.9 3,895,083 - RR42_RS18010 0.37 -0.0 3,895,083 - RR42_RS18010 0.37 -1.8 3,895,083 - RR42_RS18010 0.37 -1.4 3,895,095 - RR42_RS18010 0.40 -0.9 3,895,095 - RR42_RS18010 0.40 -0.3 3,895,232 + RR42_RS18010 0.71 -0.3 3,895,232 + RR42_RS18010 0.71 -0.3 3,895,233 - RR42_RS18010 0.71 +0.3 3,895,387 + -1.0 3,895,387 + +0.1 3,895,387 + -0.1 3,895,387 + -0.3 3,895,387 + -0.5 3,895,387 + +0.3 3,895,387 + -1.0 3,895,388 - +0.3 3,895,388 - +0.1 3,895,388 - -1.5 3,895,388 - +0.2 3,895,388 - -0.2 3,895,388 - +0.1 3,895,388 - +0.7 3,895,388 - +0.3 3,895,388 - -1.4 3,895,388 - +1.3 3,895,552 + RR42_RS18015 0.22 -0.1 3,895,552 + RR42_RS18015 0.22 +0.2 3,895,553 - RR42_RS18015 0.22 +0.3 3,895,553 - RR42_RS18015 0.22 -0.3 3,895,553 - RR42_RS18015 0.22 +0.8 3,895,553 - RR42_RS18015 0.22 +0.3 3,895,573 + RR42_RS18015 0.24 -1.1 3,895,666 + RR42_RS18015 0.34 -0.2 3,895,667 - RR42_RS18015 0.34 +0.4 3,895,687 + RR42_RS18015 0.37 +1.2 3,895,723 + RR42_RS18015 0.41 -1.3 3,895,723 + RR42_RS18015 0.41 +0.7 3,895,723 + RR42_RS18015 0.41 -1.1 3,895,724 - RR42_RS18015 0.41 +0.6 3,896,030 + RR42_RS18015 0.75 +0.6 3,896,031 - RR42_RS18015 0.75 -0.6 3,896,031 - RR42_RS18015 0.75 +1.4 3,896,116 + RR42_RS18015 0.84 -0.8 3,896,117 - RR42_RS18015 0.84 -0.4 3,896,231 + -0.1 3,896,231 + -0.2 3,896,231 + -0.1 3,896,231 + -0.1 3,896,232 - +0.7 3,896,232 - -0.9 3,896,232 - -0.3 3,896,232 - +0.2 3,896,232 - -0.3
Or see this region's nucleotide sequence