Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17630 and RR42_RS17635 are separated by 783 nucleotides RR42_RS17635 and RR42_RS17640 are separated by 23 nucleotides RR42_RS17640 and RR42_RS17645 are separated by 92 nucleotides
RR42_RS17630: RR42_RS17630 - orotidine 5'-phosphate decarboxylase, at 3,814,537 to 3,815,355
_RS17630
RR42_RS17635: RR42_RS17635 - magnesium transporter CorA, at 3,816,139 to 3,817,101
_RS17635
RR42_RS17640: RR42_RS17640 - peptidoglycan transglycosylase, at 3,817,125 to 3,817,826
_RS17640
RR42_RS17645: RR42_RS17645 - shikimate dehydrogenase, at 3,817,919 to 3,818,794
_RS17645
Position (kb)
3816
3817
3818 Strain fitness (log2 ratio)
-2
-1
0
1 at 3815.454 kb on - strand at 3815.490 kb on + strand at 3815.491 kb on - strand at 3815.491 kb on - strand at 3815.491 kb on - strand at 3815.533 kb on + strand at 3815.681 kb on + strand at 3815.681 kb on + strand at 3815.681 kb on + strand at 3815.720 kb on + strand at 3815.720 kb on + strand at 3815.721 kb on - strand at 3815.735 kb on + strand at 3815.735 kb on + strand at 3815.735 kb on + strand at 3815.736 kb on - strand at 3815.746 kb on + strand at 3815.881 kb on + strand at 3815.881 kb on + strand at 3815.882 kb on - strand at 3815.882 kb on - strand at 3815.937 kb on - strand at 3815.947 kb on + strand at 3815.947 kb on + strand at 3816.287 kb on + strand, within RR42_RS17635 at 3816.287 kb on + strand, within RR42_RS17635 at 3816.338 kb on - strand, within RR42_RS17635 at 3816.553 kb on + strand, within RR42_RS17635 at 3816.598 kb on + strand, within RR42_RS17635 at 3816.599 kb on - strand, within RR42_RS17635 at 3816.973 kb on + strand, within RR42_RS17635 at 3816.974 kb on - strand, within RR42_RS17635 at 3817.073 kb on - strand at 3817.073 kb on - strand at 3817.073 kb on - strand at 3817.207 kb on + strand, within RR42_RS17640 at 3817.207 kb on + strand, within RR42_RS17640 at 3817.207 kb on + strand, within RR42_RS17640 at 3817.207 kb on + strand, within RR42_RS17640 at 3817.207 kb on + strand, within RR42_RS17640 at 3817.207 kb on + strand, within RR42_RS17640 at 3817.208 kb on - strand, within RR42_RS17640 at 3817.208 kb on - strand, within RR42_RS17640 at 3817.300 kb on + strand, within RR42_RS17640 at 3817.300 kb on + strand, within RR42_RS17640 at 3817.301 kb on - strand, within RR42_RS17640 at 3817.301 kb on - strand, within RR42_RS17640 at 3817.301 kb on - strand, within RR42_RS17640 at 3817.360 kb on + strand, within RR42_RS17640 at 3817.361 kb on - strand, within RR42_RS17640 at 3817.361 kb on - strand, within RR42_RS17640 at 3817.373 kb on + strand, within RR42_RS17640 at 3817.373 kb on + strand, within RR42_RS17640 at 3817.373 kb on + strand, within RR42_RS17640 at 3817.373 kb on + strand, within RR42_RS17640 at 3817.374 kb on - strand, within RR42_RS17640 at 3817.374 kb on - strand, within RR42_RS17640 at 3817.374 kb on - strand, within RR42_RS17640 at 3817.439 kb on - strand, within RR42_RS17640 at 3817.439 kb on - strand, within RR42_RS17640 at 3817.508 kb on + strand, within RR42_RS17640 at 3817.508 kb on + strand, within RR42_RS17640 at 3817.508 kb on + strand, within RR42_RS17640 at 3817.508 kb on + strand, within RR42_RS17640 at 3817.509 kb on - strand, within RR42_RS17640 at 3817.628 kb on + strand, within RR42_RS17640 at 3817.628 kb on + strand, within RR42_RS17640 at 3817.629 kb on - strand, within RR42_RS17640 at 3817.629 kb on - strand, within RR42_RS17640 at 3817.629 kb on - strand, within RR42_RS17640 at 3817.629 kb on - strand, within RR42_RS17640 at 3817.636 kb on + strand, within RR42_RS17640 at 3817.637 kb on - strand, within RR42_RS17640 at 3817.765 kb on + strand at 3817.765 kb on + strand at 3817.765 kb on + strand at 3817.765 kb on + strand at 3817.766 kb on - strand at 3817.810 kb on + strand at 3818.001 kb on + strand at 3818.001 kb on + strand at 3818.001 kb on + strand at 3818.001 kb on + strand at 3818.002 kb on - strand at 3818.084 kb on + strand, within RR42_RS17645 at 3818.084 kb on + strand, within RR42_RS17645 at 3818.085 kb on - strand, within RR42_RS17645 at 3818.085 kb on - strand, within RR42_RS17645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,815,454 - +0.3 3,815,490 + -0.5 3,815,491 - -0.0 3,815,491 - +0.5 3,815,491 - +0.4 3,815,533 + +0.2 3,815,681 + -0.9 3,815,681 + +0.3 3,815,681 + -1.0 3,815,720 + -0.2 3,815,720 + -1.0 3,815,721 - +1.3 3,815,735 + -1.7 3,815,735 + +0.6 3,815,735 + +0.4 3,815,736 - +1.0 3,815,746 + -0.0 3,815,881 + -0.1 3,815,881 + +1.1 3,815,882 - +0.5 3,815,882 - -0.1 3,815,937 - +0.1 3,815,947 + -0.1 3,815,947 + -0.0 3,816,287 + RR42_RS17635 0.15 -0.7 3,816,287 + RR42_RS17635 0.15 +0.0 3,816,338 - RR42_RS17635 0.21 -0.6 3,816,553 + RR42_RS17635 0.43 +0.7 3,816,598 + RR42_RS17635 0.48 +1.4 3,816,599 - RR42_RS17635 0.48 +1.2 3,816,973 + RR42_RS17635 0.87 -0.5 3,816,974 - RR42_RS17635 0.87 +0.8 3,817,073 - -0.7 3,817,073 - -0.8 3,817,073 - +0.4 3,817,207 + RR42_RS17640 0.12 +0.2 3,817,207 + RR42_RS17640 0.12 +0.2 3,817,207 + RR42_RS17640 0.12 -0.4 3,817,207 + RR42_RS17640 0.12 -0.2 3,817,207 + RR42_RS17640 0.12 -1.0 3,817,207 + RR42_RS17640 0.12 -0.0 3,817,208 - RR42_RS17640 0.12 +1.0 3,817,208 - RR42_RS17640 0.12 -1.4 3,817,300 + RR42_RS17640 0.25 +0.1 3,817,300 + RR42_RS17640 0.25 +0.1 3,817,301 - RR42_RS17640 0.25 -0.3 3,817,301 - RR42_RS17640 0.25 -0.1 3,817,301 - RR42_RS17640 0.25 +0.1 3,817,360 + RR42_RS17640 0.33 +1.1 3,817,361 - RR42_RS17640 0.34 -1.2 3,817,361 - RR42_RS17640 0.34 +0.9 3,817,373 + RR42_RS17640 0.35 -0.3 3,817,373 + RR42_RS17640 0.35 -0.3 3,817,373 + RR42_RS17640 0.35 -0.7 3,817,373 + RR42_RS17640 0.35 -0.7 3,817,374 - RR42_RS17640 0.35 -2.4 3,817,374 - RR42_RS17640 0.35 +0.8 3,817,374 - RR42_RS17640 0.35 +0.2 3,817,439 - RR42_RS17640 0.45 +0.0 3,817,439 - RR42_RS17640 0.45 +1.1 3,817,508 + RR42_RS17640 0.55 +1.5 3,817,508 + RR42_RS17640 0.55 +0.2 3,817,508 + RR42_RS17640 0.55 +0.2 3,817,508 + RR42_RS17640 0.55 +0.1 3,817,509 - RR42_RS17640 0.55 +0.1 3,817,628 + RR42_RS17640 0.72 -0.7 3,817,628 + RR42_RS17640 0.72 -1.1 3,817,629 - RR42_RS17640 0.72 -0.4 3,817,629 - RR42_RS17640 0.72 -1.1 3,817,629 - RR42_RS17640 0.72 -0.1 3,817,629 - RR42_RS17640 0.72 -0.8 3,817,636 + RR42_RS17640 0.73 +1.5 3,817,637 - RR42_RS17640 0.73 -0.0 3,817,765 + -1.4 3,817,765 + +0.1 3,817,765 + -0.3 3,817,765 + +0.2 3,817,766 - +0.3 3,817,810 + -0.3 3,818,001 + -0.7 3,818,001 + -0.2 3,818,001 + -0.0 3,818,001 + -0.7 3,818,002 - -0.3 3,818,084 + RR42_RS17645 0.19 -0.7 3,818,084 + RR42_RS17645 0.19 +0.9 3,818,085 - RR42_RS17645 0.19 -0.6 3,818,085 - RR42_RS17645 0.19 +1.1
Or see this region's nucleotide sequence