Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16530 and RR42_RS16535 are separated by 36 nucleotides RR42_RS16535 and RR42_RS16540 are separated by 264 nucleotides
RR42_RS16530: RR42_RS16530 - metallophosphoesterase, at 3,580,760 to 3,581,704
_RS16530
RR42_RS16535: RR42_RS16535 - hypothetical protein, at 3,581,741 to 3,582,088
_RS16535
RR42_RS16540: RR42_RS16540 - penicillin-binding protein, at 3,582,353 to 3,584,542
_RS16540
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-2
-1
0
1 at 3580.759 kb on + strand at 3580.759 kb on + strand at 3580.759 kb on + strand at 3580.952 kb on - strand, within RR42_RS16530 at 3581.065 kb on + strand, within RR42_RS16530 at 3581.066 kb on - strand, within RR42_RS16530 at 3581.074 kb on - strand, within RR42_RS16530 at 3581.074 kb on - strand, within RR42_RS16530 at 3581.126 kb on - strand, within RR42_RS16530 at 3581.140 kb on + strand, within RR42_RS16530 at 3581.186 kb on + strand, within RR42_RS16530 at 3581.228 kb on + strand, within RR42_RS16530 at 3581.228 kb on + strand, within RR42_RS16530 at 3581.405 kb on + strand, within RR42_RS16530 at 3581.406 kb on - strand, within RR42_RS16530 at 3581.453 kb on + strand, within RR42_RS16530 at 3581.454 kb on - strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.528 kb on - strand, within RR42_RS16530 at 3581.540 kb on - strand, within RR42_RS16530 at 3581.745 kb on + strand at 3582.003 kb on - strand, within RR42_RS16535 at 3582.033 kb on - strand, within RR42_RS16535 at 3582.051 kb on + strand, within RR42_RS16535 at 3582.061 kb on + strand at 3582.232 kb on + strand at 3582.232 kb on + strand at 3582.233 kb on - strand at 3582.239 kb on - strand at 3582.242 kb on + strand at 3582.242 kb on + strand at 3582.242 kb on + strand at 3582.243 kb on - strand at 3582.298 kb on - strand at 3582.298 kb on - strand at 3582.429 kb on + strand at 3582.429 kb on + strand at 3582.521 kb on + strand at 3582.521 kb on + strand at 3582.521 kb on + strand at 3582.522 kb on - strand at 3582.522 kb on - strand at 3582.625 kb on + strand, within RR42_RS16540 at 3582.748 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.911 kb on + strand, within RR42_RS16540 at 3582.962 kb on + strand, within RR42_RS16540 at 3582.998 kb on + strand, within RR42_RS16540 at 3582.999 kb on - strand, within RR42_RS16540 at 3582.999 kb on - strand, within RR42_RS16540 at 3583.076 kb on + strand, within RR42_RS16540 at 3583.077 kb on - strand, within RR42_RS16540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,580,759 + -0.3 3,580,759 + -0.5 3,580,759 + -0.6 3,580,952 - RR42_RS16530 0.20 +0.3 3,581,065 + RR42_RS16530 0.32 -0.3 3,581,066 - RR42_RS16530 0.32 +1.0 3,581,074 - RR42_RS16530 0.33 -0.6 3,581,074 - RR42_RS16530 0.33 +0.3 3,581,126 - RR42_RS16530 0.39 -1.0 3,581,140 + RR42_RS16530 0.40 +0.5 3,581,186 + RR42_RS16530 0.45 +0.1 3,581,228 + RR42_RS16530 0.50 -1.1 3,581,228 + RR42_RS16530 0.50 -0.3 3,581,405 + RR42_RS16530 0.68 +0.3 3,581,406 - RR42_RS16530 0.68 -1.2 3,581,453 + RR42_RS16530 0.73 +0.1 3,581,454 - RR42_RS16530 0.73 -0.4 3,581,527 + RR42_RS16530 0.81 +0.4 3,581,527 + RR42_RS16530 0.81 +0.2 3,581,527 + RR42_RS16530 0.81 -1.6 3,581,528 - RR42_RS16530 0.81 -0.3 3,581,540 - RR42_RS16530 0.83 -1.2 3,581,745 + -0.4 3,582,003 - RR42_RS16535 0.75 +1.4 3,582,033 - RR42_RS16535 0.84 +1.1 3,582,051 + RR42_RS16535 0.89 +1.2 3,582,061 + +0.5 3,582,232 + -0.4 3,582,232 + -0.6 3,582,233 - +1.2 3,582,239 - -0.3 3,582,242 + -2.5 3,582,242 + +0.8 3,582,242 + +1.2 3,582,243 - +0.5 3,582,298 - +0.1 3,582,298 - -0.1 3,582,429 + -1.4 3,582,429 + -0.8 3,582,521 + -0.8 3,582,521 + +0.7 3,582,521 + -2.2 3,582,522 - +0.5 3,582,522 - -0.2 3,582,625 + RR42_RS16540 0.12 +1.3 3,582,748 + RR42_RS16540 0.18 -1.3 3,582,769 + RR42_RS16540 0.19 -1.5 3,582,769 + RR42_RS16540 0.19 +1.1 3,582,769 + RR42_RS16540 0.19 -0.2 3,582,769 + RR42_RS16540 0.19 -0.6 3,582,911 + RR42_RS16540 0.25 -0.7 3,582,962 + RR42_RS16540 0.28 -0.0 3,582,998 + RR42_RS16540 0.29 +0.6 3,582,999 - RR42_RS16540 0.29 -1.8 3,582,999 - RR42_RS16540 0.29 -0.9 3,583,076 + RR42_RS16540 0.33 -2.0 3,583,077 - RR42_RS16540 0.33 -0.5
Or see this region's nucleotide sequence