Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14980 and RR42_RS14985 are separated by 130 nucleotides RR42_RS14985 and RR42_RS14990 are separated by 111 nucleotides RR42_RS14990 and RR42_RS14995 are separated by 184 nucleotides
RR42_RS14980: RR42_RS14980 - glutamyl-tRNA amidotransferase, at 3,239,567 to 3,240,016
_RS14980
RR42_RS14985: RR42_RS14985 - 30S ribosomal protein S21, at 3,240,147 to 3,240,359
_RS14985
RR42_RS14990: RR42_RS14990 - membrane protein, at 3,240,471 to 3,241,745
_RS14990
RR42_RS14995: RR42_RS14995 - tRNA threonylcarbamoyladenosine modification protein TsaD, at 3,241,930 to 3,242,970
_RS14995
Position (kb)
3240
3241
3242 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3239.513 kb on + strand at 3239.551 kb on + strand at 3239.660 kb on - strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.752 kb on - strand, within RR42_RS14980 at 3240.022 kb on + strand at 3240.152 kb on - strand at 3240.152 kb on - strand at 3240.191 kb on - strand, within RR42_RS14985 at 3240.371 kb on - strand at 3240.403 kb on - strand at 3240.403 kb on - strand at 3240.410 kb on + strand at 3240.433 kb on + strand at 3240.557 kb on + strand at 3240.557 kb on + strand at 3240.558 kb on - strand at 3240.559 kb on + strand at 3240.560 kb on - strand at 3240.604 kb on + strand, within RR42_RS14990 at 3240.604 kb on + strand, within RR42_RS14990 at 3240.767 kb on + strand, within RR42_RS14990 at 3240.767 kb on + strand, within RR42_RS14990 at 3241.471 kb on + strand, within RR42_RS14990 at 3241.471 kb on + strand, within RR42_RS14990 at 3241.774 kb on - strand at 3241.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,239,513 + -0.1 3,239,551 + +0.3 3,239,660 - RR42_RS14980 0.21 -1.0 3,239,751 + RR42_RS14980 0.41 -1.2 3,239,751 + RR42_RS14980 0.41 -0.8 3,239,751 + RR42_RS14980 0.41 -0.2 3,239,752 - RR42_RS14980 0.41 -1.0 3,240,022 + +0.0 3,240,152 - +0.7 3,240,152 - +1.0 3,240,191 - RR42_RS14985 0.21 -1.2 3,240,371 - +0.0 3,240,403 - -0.3 3,240,403 - -0.5 3,240,410 + -0.6 3,240,433 + +1.1 3,240,557 + -1.3 3,240,557 + -0.1 3,240,558 - +0.2 3,240,559 + -0.0 3,240,560 - -0.1 3,240,604 + RR42_RS14990 0.10 -0.1 3,240,604 + RR42_RS14990 0.10 -0.5 3,240,767 + RR42_RS14990 0.23 -0.5 3,240,767 + RR42_RS14990 0.23 -2.7 3,241,471 + RR42_RS14990 0.78 -0.4 3,241,471 + RR42_RS14990 0.78 +0.8 3,241,774 - -2.9 3,241,830 + -0.2
Or see this region's nucleotide sequence