Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14735

Experiment: R2A with Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14730 and RR42_RS14735 are separated by 564 nucleotidesRR42_RS14735 and RR42_RS14740 are separated by 187 nucleotidesRR42_RS14740 and RR42_RS14745 overlap by 4 nucleotides RR42_RS14730: RR42_RS14730 - methyltransferase, at 3,174,925 to 3,176,025 _RS14730 RR42_RS14735: RR42_RS14735 - membrane protein, at 3,176,590 to 3,177,288 _RS14735 RR42_RS14740: RR42_RS14740 - thymidylate synthase, at 3,177,476 to 3,178,270 _RS14740 RR42_RS14745: RR42_RS14745 - diacylglycerol kinase, at 3,178,267 to 3,178,767 _RS14745 Position (kb) 3176 3177 3178Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3175.712 kb on + strand, within RR42_RS14730at 3175.712 kb on + strand, within RR42_RS14730at 3175.715 kb on + strand, within RR42_RS14730at 3175.715 kb on + strand, within RR42_RS14730at 3175.766 kb on + strand, within RR42_RS14730at 3175.767 kb on - strand, within RR42_RS14730at 3175.767 kb on - strand, within RR42_RS14730at 3175.767 kb on - strand, within RR42_RS14730at 3175.767 kb on - strand, within RR42_RS14730at 3175.987 kb on + strandat 3175.988 kb on - strandat 3175.988 kb on - strandat 3176.057 kb on + strandat 3176.057 kb on + strandat 3176.058 kb on - strandat 3176.058 kb on - strandat 3176.058 kb on - strandat 3176.058 kb on - strandat 3176.058 kb on - strandat 3176.298 kb on + strandat 3176.298 kb on + strandat 3176.299 kb on - strandat 3176.312 kb on + strandat 3176.314 kb on + strandat 3176.450 kb on + strandat 3176.550 kb on + strandat 3176.563 kb on + strandat 3176.567 kb on + strandat 3176.567 kb on + strandat 3177.199 kb on + strand, within RR42_RS14735at 3177.200 kb on - strand, within RR42_RS14735at 3177.200 kb on - strand, within RR42_RS14735at 3177.200 kb on - strand, within RR42_RS14735at 3177.286 kb on + strandat 3177.316 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM
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3,175,712 + RR42_RS14730 0.71 +0.2
3,175,712 + RR42_RS14730 0.71 +0.3
3,175,715 + RR42_RS14730 0.72 +0.3
3,175,715 + RR42_RS14730 0.72 -0.1
3,175,766 + RR42_RS14730 0.76 +0.8
3,175,767 - RR42_RS14730 0.76 -0.3
3,175,767 - RR42_RS14730 0.76 -1.7
3,175,767 - RR42_RS14730 0.76 +0.7
3,175,767 - RR42_RS14730 0.76 -0.1
3,175,987 + -1.0
3,175,988 - -0.3
3,175,988 - -0.0
3,176,057 + -1.1
3,176,057 + +0.6
3,176,058 - -0.6
3,176,058 - -0.6
3,176,058 - +2.9
3,176,058 - +0.1
3,176,058 - -0.3
3,176,298 + -0.2
3,176,298 + -2.6
3,176,299 - +0.1
3,176,312 + -0.3
3,176,314 + +0.2
3,176,450 + -1.1
3,176,550 + +1.6
3,176,563 + +0.1
3,176,567 + -1.2
3,176,567 + -0.3
3,177,199 + RR42_RS14735 0.87 +0.5
3,177,200 - RR42_RS14735 0.87 -0.1
3,177,200 - RR42_RS14735 0.87 +1.3
3,177,200 - RR42_RS14735 0.87 +1.1
3,177,286 + +1.8
3,177,316 + +1.6

Or see this region's nucleotide sequence