Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14525

Experiment: R2A with Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14520 and RR42_RS14525 are separated by 93 nucleotidesRR42_RS14525 and RR42_RS14530 are separated by 110 nucleotidesRR42_RS14530 and RR42_RS14535 are separated by 457 nucleotides RR42_RS14520: RR42_RS14520 - mobilization protein, at 3,125,517 to 3,126,212 _RS14520 RR42_RS14525: RR42_RS14525 - cysteine synthase, at 3,126,306 to 3,127,442 _RS14525 RR42_RS14530: RR42_RS14530 - 2-hydroxyacid dehydrogenase, at 3,127,553 to 3,127,948 _RS14530 RR42_RS14535: RR42_RS14535 - hypothetical protein, at 3,128,406 to 3,129,551 _RS14535 Position (kb) 3126 3127 3128Strain fitness (log2 ratio) -2 -1 0 1 2at 3125.368 kb on - strandat 3125.368 kb on - strandat 3125.374 kb on + strandat 3125.375 kb on - strandat 3125.393 kb on + strandat 3125.398 kb on + strandat 3125.415 kb on + strandat 3125.415 kb on + strandat 3125.415 kb on + strandat 3125.416 kb on - strandat 3125.416 kb on - strandat 3125.516 kb on + strandat 3125.523 kb on + strandat 3125.523 kb on + strandat 3125.523 kb on + strandat 3125.523 kb on + strandat 3125.584 kb on + strandat 3125.664 kb on + strand, within RR42_RS14520at 3125.665 kb on - strand, within RR42_RS14520at 3126.311 kb on + strandat 3126.312 kb on - strandat 3126.439 kb on + strand, within RR42_RS14525at 3126.455 kb on - strand, within RR42_RS14525at 3126.468 kb on - strand, within RR42_RS14525at 3126.469 kb on + strand, within RR42_RS14525at 3126.674 kb on + strand, within RR42_RS14525at 3126.730 kb on - strand, within RR42_RS14525at 3126.776 kb on - strand, within RR42_RS14525at 3126.825 kb on - strand, within RR42_RS14525at 3126.825 kb on - strand, within RR42_RS14525at 3126.836 kb on - strand, within RR42_RS14525at 3126.950 kb on + strand, within RR42_RS14525at 3126.988 kb on + strand, within RR42_RS14525at 3126.989 kb on - strand, within RR42_RS14525at 3127.032 kb on - strand, within RR42_RS14525at 3127.048 kb on + strand, within RR42_RS14525at 3127.049 kb on - strand, within RR42_RS14525at 3127.091 kb on - strand, within RR42_RS14525at 3127.174 kb on + strand, within RR42_RS14525at 3127.458 kb on - strandat 3127.507 kb on - strandat 3127.644 kb on + strand, within RR42_RS14530at 3127.644 kb on + strand, within RR42_RS14530at 3127.645 kb on - strand, within RR42_RS14530at 3127.765 kb on + strand, within RR42_RS14530at 3127.766 kb on - strand, within RR42_RS14530at 3127.858 kb on - strand, within RR42_RS14530at 3127.858 kb on - strand, within RR42_RS14530at 3127.977 kb on + strandat 3127.977 kb on + strandat 3128.019 kb on - strandat 3128.055 kb on + strandat 3128.059 kb on - strandat 3128.061 kb on + strandat 3128.062 kb on - strandat 3128.062 kb on - strandat 3128.062 kb on - strandat 3128.192 kb on + strandat 3128.193 kb on - strandat 3128.346 kb on - strandat 3128.424 kb on + strandat 3128.424 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM
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3,125,368 - +0.1
3,125,368 - -0.6
3,125,374 + +0.5
3,125,375 - -0.1
3,125,393 + -1.2
3,125,398 + -0.4
3,125,415 + +1.9
3,125,415 + +0.9
3,125,415 + -0.0
3,125,416 - +1.5
3,125,416 - -0.9
3,125,516 + -0.1
3,125,523 + +1.9
3,125,523 + -0.6
3,125,523 + -0.4
3,125,523 + -0.4
3,125,584 + -0.1
3,125,664 + RR42_RS14520 0.21 +0.0
3,125,665 - RR42_RS14520 0.21 -0.0
3,126,311 + -0.5
3,126,312 - -1.1
3,126,439 + RR42_RS14525 0.12 +0.8
3,126,455 - RR42_RS14525 0.13 +0.2
3,126,468 - RR42_RS14525 0.14 +0.5
3,126,469 + RR42_RS14525 0.14 +1.5
3,126,674 + RR42_RS14525 0.32 +1.0
3,126,730 - RR42_RS14525 0.37 +0.9
3,126,776 - RR42_RS14525 0.41 +0.7
3,126,825 - RR42_RS14525 0.46 +0.6
3,126,825 - RR42_RS14525 0.46 -1.7
3,126,836 - RR42_RS14525 0.47 +0.6
3,126,950 + RR42_RS14525 0.57 +0.2
3,126,988 + RR42_RS14525 0.60 +0.2
3,126,989 - RR42_RS14525 0.60 -1.7
3,127,032 - RR42_RS14525 0.64 -0.2
3,127,048 + RR42_RS14525 0.65 +1.0
3,127,049 - RR42_RS14525 0.65 -0.1
3,127,091 - RR42_RS14525 0.69 +0.3
3,127,174 + RR42_RS14525 0.76 +0.5
3,127,458 - -1.4
3,127,507 - -0.4
3,127,644 + RR42_RS14530 0.23 -1.3
3,127,644 + RR42_RS14530 0.23 +0.3
3,127,645 - RR42_RS14530 0.23 -0.4
3,127,765 + RR42_RS14530 0.54 -1.3
3,127,766 - RR42_RS14530 0.54 -0.2
3,127,858 - RR42_RS14530 0.77 -1.5
3,127,858 - RR42_RS14530 0.77 -2.1
3,127,977 + +0.3
3,127,977 + +1.9
3,128,019 - -0.8
3,128,055 + +0.5
3,128,059 - -1.1
3,128,061 + +0.1
3,128,062 - -0.2
3,128,062 - -1.0
3,128,062 - +0.4
3,128,192 + -0.8
3,128,193 - -0.9
3,128,346 - -0.4
3,128,424 + +0.1
3,128,424 + -0.7

Or see this region's nucleotide sequence