Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14520 and RR42_RS14525 are separated by 93 nucleotides RR42_RS14525 and RR42_RS14530 are separated by 110 nucleotides RR42_RS14530 and RR42_RS14535 are separated by 457 nucleotides
RR42_RS14520: RR42_RS14520 - mobilization protein, at 3,125,517 to 3,126,212
_RS14520
RR42_RS14525: RR42_RS14525 - cysteine synthase, at 3,126,306 to 3,127,442
_RS14525
RR42_RS14530: RR42_RS14530 - 2-hydroxyacid dehydrogenase, at 3,127,553 to 3,127,948
_RS14530
RR42_RS14535: RR42_RS14535 - hypothetical protein, at 3,128,406 to 3,129,551
_RS14535
Position (kb)
3126
3127
3128 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3125.368 kb on - strand at 3125.368 kb on - strand at 3125.374 kb on + strand at 3125.375 kb on - strand at 3125.393 kb on + strand at 3125.398 kb on + strand at 3125.415 kb on + strand at 3125.415 kb on + strand at 3125.415 kb on + strand at 3125.416 kb on - strand at 3125.416 kb on - strand at 3125.516 kb on + strand at 3125.523 kb on + strand at 3125.523 kb on + strand at 3125.523 kb on + strand at 3125.523 kb on + strand at 3125.584 kb on + strand at 3125.664 kb on + strand, within RR42_RS14520 at 3125.665 kb on - strand, within RR42_RS14520 at 3126.311 kb on + strand at 3126.312 kb on - strand at 3126.439 kb on + strand, within RR42_RS14525 at 3126.455 kb on - strand, within RR42_RS14525 at 3126.468 kb on - strand, within RR42_RS14525 at 3126.469 kb on + strand, within RR42_RS14525 at 3126.674 kb on + strand, within RR42_RS14525 at 3126.730 kb on - strand, within RR42_RS14525 at 3126.776 kb on - strand, within RR42_RS14525 at 3126.825 kb on - strand, within RR42_RS14525 at 3126.825 kb on - strand, within RR42_RS14525 at 3126.836 kb on - strand, within RR42_RS14525 at 3126.950 kb on + strand, within RR42_RS14525 at 3126.988 kb on + strand, within RR42_RS14525 at 3126.989 kb on - strand, within RR42_RS14525 at 3127.032 kb on - strand, within RR42_RS14525 at 3127.048 kb on + strand, within RR42_RS14525 at 3127.049 kb on - strand, within RR42_RS14525 at 3127.091 kb on - strand, within RR42_RS14525 at 3127.174 kb on + strand, within RR42_RS14525 at 3127.458 kb on - strand at 3127.507 kb on - strand at 3127.644 kb on + strand, within RR42_RS14530 at 3127.644 kb on + strand, within RR42_RS14530 at 3127.645 kb on - strand, within RR42_RS14530 at 3127.765 kb on + strand, within RR42_RS14530 at 3127.766 kb on - strand, within RR42_RS14530 at 3127.858 kb on - strand, within RR42_RS14530 at 3127.858 kb on - strand, within RR42_RS14530 at 3127.977 kb on + strand at 3127.977 kb on + strand at 3128.019 kb on - strand at 3128.055 kb on + strand at 3128.059 kb on - strand at 3128.061 kb on + strand at 3128.062 kb on - strand at 3128.062 kb on - strand at 3128.062 kb on - strand at 3128.192 kb on + strand at 3128.193 kb on - strand at 3128.346 kb on - strand at 3128.424 kb on + strand at 3128.424 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,125,368 - +0.1 3,125,368 - -0.6 3,125,374 + +0.5 3,125,375 - -0.1 3,125,393 + -1.2 3,125,398 + -0.4 3,125,415 + +1.9 3,125,415 + +0.9 3,125,415 + -0.0 3,125,416 - +1.5 3,125,416 - -0.9 3,125,516 + -0.1 3,125,523 + +1.9 3,125,523 + -0.6 3,125,523 + -0.4 3,125,523 + -0.4 3,125,584 + -0.1 3,125,664 + RR42_RS14520 0.21 +0.0 3,125,665 - RR42_RS14520 0.21 -0.0 3,126,311 + -0.5 3,126,312 - -1.1 3,126,439 + RR42_RS14525 0.12 +0.8 3,126,455 - RR42_RS14525 0.13 +0.2 3,126,468 - RR42_RS14525 0.14 +0.5 3,126,469 + RR42_RS14525 0.14 +1.5 3,126,674 + RR42_RS14525 0.32 +1.0 3,126,730 - RR42_RS14525 0.37 +0.9 3,126,776 - RR42_RS14525 0.41 +0.7 3,126,825 - RR42_RS14525 0.46 +0.6 3,126,825 - RR42_RS14525 0.46 -1.7 3,126,836 - RR42_RS14525 0.47 +0.6 3,126,950 + RR42_RS14525 0.57 +0.2 3,126,988 + RR42_RS14525 0.60 +0.2 3,126,989 - RR42_RS14525 0.60 -1.7 3,127,032 - RR42_RS14525 0.64 -0.2 3,127,048 + RR42_RS14525 0.65 +1.0 3,127,049 - RR42_RS14525 0.65 -0.1 3,127,091 - RR42_RS14525 0.69 +0.3 3,127,174 + RR42_RS14525 0.76 +0.5 3,127,458 - -1.4 3,127,507 - -0.4 3,127,644 + RR42_RS14530 0.23 -1.3 3,127,644 + RR42_RS14530 0.23 +0.3 3,127,645 - RR42_RS14530 0.23 -0.4 3,127,765 + RR42_RS14530 0.54 -1.3 3,127,766 - RR42_RS14530 0.54 -0.2 3,127,858 - RR42_RS14530 0.77 -1.5 3,127,858 - RR42_RS14530 0.77 -2.1 3,127,977 + +0.3 3,127,977 + +1.9 3,128,019 - -0.8 3,128,055 + +0.5 3,128,059 - -1.1 3,128,061 + +0.1 3,128,062 - -0.2 3,128,062 - -1.0 3,128,062 - +0.4 3,128,192 + -0.8 3,128,193 - -0.9 3,128,346 - -0.4 3,128,424 + +0.1 3,128,424 + -0.7
Or see this region's nucleotide sequence