Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14495 and RR42_RS14500 are separated by 172 nucleotides RR42_RS14500 and RR42_RS14505 are separated by 294 nucleotides
RR42_RS14495: RR42_RS14495 - hypothetical protein, at 3,119,530 to 3,120,732
_RS14495
RR42_RS14500: RR42_RS14500 - SAM-dependent methyltransferase, at 3,120,905 to 3,121,588
_RS14500
RR42_RS14505: RR42_RS14505 - ABC transporter substrate-binding protein, at 3,121,883 to 3,123,469
_RS14505
Position (kb)
3120
3121
3122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3119.965 kb on + strand, within RR42_RS14495 at 3119.971 kb on + strand, within RR42_RS14495 at 3119.971 kb on + strand, within RR42_RS14495 at 3119.972 kb on - strand, within RR42_RS14495 at 3119.972 kb on - strand, within RR42_RS14495 at 3120.022 kb on + strand, within RR42_RS14495 at 3120.022 kb on + strand, within RR42_RS14495 at 3120.023 kb on - strand, within RR42_RS14495 at 3120.359 kb on - strand, within RR42_RS14495 at 3120.359 kb on - strand, within RR42_RS14495 at 3120.439 kb on + strand, within RR42_RS14495 at 3120.440 kb on - strand, within RR42_RS14495 at 3120.447 kb on - strand, within RR42_RS14495 at 3120.482 kb on - strand, within RR42_RS14495 at 3120.502 kb on + strand, within RR42_RS14495 at 3120.731 kb on - strand at 3120.731 kb on - strand at 3120.960 kb on + strand at 3120.960 kb on + strand at 3121.058 kb on - strand, within RR42_RS14500 at 3121.058 kb on - strand, within RR42_RS14500 at 3121.058 kb on - strand, within RR42_RS14500 at 3121.059 kb on + strand, within RR42_RS14500 at 3121.059 kb on + strand, within RR42_RS14500 at 3121.060 kb on - strand, within RR42_RS14500 at 3121.060 kb on - strand, within RR42_RS14500 at 3121.090 kb on - strand, within RR42_RS14500 at 3121.176 kb on + strand, within RR42_RS14500 at 3121.185 kb on + strand, within RR42_RS14500 at 3121.185 kb on + strand, within RR42_RS14500 at 3121.186 kb on - strand, within RR42_RS14500 at 3121.591 kb on + strand at 3121.676 kb on + strand at 3121.677 kb on - strand at 3121.677 kb on - strand at 3121.682 kb on + strand at 3121.682 kb on + strand at 3121.683 kb on - strand at 3121.683 kb on - strand at 3121.683 kb on - strand at 3121.817 kb on + strand at 3121.818 kb on - strand at 3122.062 kb on + strand, within RR42_RS14505 at 3122.062 kb on + strand, within RR42_RS14505 at 3122.063 kb on - strand, within RR42_RS14505 at 3122.063 kb on - strand, within RR42_RS14505 at 3122.103 kb on - strand, within RR42_RS14505 at 3122.273 kb on + strand, within RR42_RS14505 at 3122.345 kb on + strand, within RR42_RS14505 at 3122.346 kb on - strand, within RR42_RS14505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,119,965 + RR42_RS14495 0.36 -1.5 3,119,971 + RR42_RS14495 0.37 -1.7 3,119,971 + RR42_RS14495 0.37 -0.3 3,119,972 - RR42_RS14495 0.37 +2.2 3,119,972 - RR42_RS14495 0.37 -0.6 3,120,022 + RR42_RS14495 0.41 -1.7 3,120,022 + RR42_RS14495 0.41 -2.3 3,120,023 - RR42_RS14495 0.41 -0.1 3,120,359 - RR42_RS14495 0.69 +0.6 3,120,359 - RR42_RS14495 0.69 +0.7 3,120,439 + RR42_RS14495 0.76 -0.3 3,120,440 - RR42_RS14495 0.76 -0.2 3,120,447 - RR42_RS14495 0.76 -1.7 3,120,482 - RR42_RS14495 0.79 -0.4 3,120,502 + RR42_RS14495 0.81 -0.0 3,120,731 - +0.6 3,120,731 - -0.1 3,120,960 + -0.3 3,120,960 + -0.1 3,121,058 - RR42_RS14500 0.22 +0.3 3,121,058 - RR42_RS14500 0.22 +1.8 3,121,058 - RR42_RS14500 0.22 +0.2 3,121,059 + RR42_RS14500 0.23 +0.8 3,121,059 + RR42_RS14500 0.23 +0.4 3,121,060 - RR42_RS14500 0.23 +0.4 3,121,060 - RR42_RS14500 0.23 +0.6 3,121,090 - RR42_RS14500 0.27 -1.0 3,121,176 + RR42_RS14500 0.40 -0.1 3,121,185 + RR42_RS14500 0.41 +1.7 3,121,185 + RR42_RS14500 0.41 -0.8 3,121,186 - RR42_RS14500 0.41 +1.2 3,121,591 + +2.2 3,121,676 + -0.5 3,121,677 - +0.8 3,121,677 - -0.3 3,121,682 + +0.5 3,121,682 + -0.3 3,121,683 - +0.2 3,121,683 - -0.0 3,121,683 - +0.6 3,121,817 + -0.4 3,121,818 - -0.5 3,122,062 + RR42_RS14505 0.11 -1.9 3,122,062 + RR42_RS14505 0.11 -0.2 3,122,063 - RR42_RS14505 0.11 -0.0 3,122,063 - RR42_RS14505 0.11 -0.4 3,122,103 - RR42_RS14505 0.14 -0.6 3,122,273 + RR42_RS14505 0.25 -2.3 3,122,345 + RR42_RS14505 0.29 +0.2 3,122,346 - RR42_RS14505 0.29 +0.4
Or see this region's nucleotide sequence