Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13035 and RR42_RS13040 are separated by 110 nucleotides RR42_RS13040 and RR42_RS13045 are separated by 99 nucleotides RR42_RS13045 and RR42_RS13050 are separated by 174 nucleotides
RR42_RS13035: RR42_RS13035 - amino acid acetyltransferase, at 2,808,618 to 2,810,054
_RS13035
RR42_RS13040: RR42_RS13040 - iron transporter, at 2,810,165 to 2,810,440
_RS13040
RR42_RS13045: RR42_RS13045 - ribose-5-phosphate isomerase A, at 2,810,540 to 2,811,226
_RS13045
RR42_RS13050: RR42_RS13050 - transaldolase, at 2,811,401 to 2,812,339
_RS13050
Position (kb)
2810
2811 Strain fitness (log2 ratio)
-1
0
1 at 2810.094 kb on + strand at 2810.094 kb on + strand at 2810.095 kb on - strand at 2810.095 kb on - strand at 2810.099 kb on + strand at 2810.137 kb on + strand at 2810.138 kb on - strand at 2810.381 kb on + strand, within RR42_RS13040 at 2810.381 kb on + strand, within RR42_RS13040 at 2810.382 kb on - strand, within RR42_RS13040 at 2810.417 kb on + strand at 2810.418 kb on - strand at 2810.418 kb on - strand at 2810.418 kb on - strand at 2811.261 kb on - strand at 2811.308 kb on + strand at 2811.309 kb on + strand at 2811.309 kb on + strand at 2811.310 kb on - strand at 2811.310 kb on - strand at 2811.382 kb on + strand at 2811.382 kb on + strand at 2811.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 2,810,094 + -1.0 2,810,094 + -1.2 2,810,095 - +0.3 2,810,095 - +0.7 2,810,099 + -0.3 2,810,137 + +1.3 2,810,138 - -0.4 2,810,381 + RR42_RS13040 0.78 -1.7 2,810,381 + RR42_RS13040 0.78 -0.3 2,810,382 - RR42_RS13040 0.79 -1.3 2,810,417 + -0.8 2,810,418 - +0.2 2,810,418 - -0.6 2,810,418 - -0.8 2,811,261 - +0.1 2,811,308 + +0.3 2,811,309 + +0.1 2,811,309 + -1.1 2,811,310 - +0.3 2,811,310 - -0.6 2,811,382 + -0.1 2,811,382 + -1.1 2,811,383 - -1.0
Or see this region's nucleotide sequence