Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS10515

Experiment: R2A with Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS10510 and RR42_RS10515 are separated by 31 nucleotidesRR42_RS10515 and RR42_RS10520 are separated by 47 nucleotidesRR42_RS10520 and RR42_RS10525 are separated by 116 nucleotides RR42_RS10510: RR42_RS10510 - dihydrofolate reductase, at 2,247,105 to 2,248,058 _RS10510 RR42_RS10515: RR42_RS10515 - fumarylacetoacetate hydrolase, at 2,248,090 to 2,248,770 _RS10515 RR42_RS10520: RR42_RS10520 - 3-oxoacyl-ACP reductase, at 2,248,818 to 2,249,576 _RS10520 RR42_RS10525: RR42_RS10525 - ABC transporter substrate-binding protein, at 2,249,693 to 2,250,652 _RS10525 Position (kb) 2248 2249Strain fitness (log2 ratio) -1 0 1at 2247.174 kb on + strandat 2247.175 kb on - strandat 2247.175 kb on - strandat 2247.190 kb on - strandat 2247.266 kb on + strand, within RR42_RS10510at 2247.358 kb on - strand, within RR42_RS10510at 2247.358 kb on - strand, within RR42_RS10510at 2247.631 kb on - strand, within RR42_RS10510at 2247.632 kb on + strand, within RR42_RS10510at 2247.633 kb on - strand, within RR42_RS10510at 2248.071 kb on - strandat 2248.192 kb on + strand, within RR42_RS10515at 2248.373 kb on - strand, within RR42_RS10515at 2248.396 kb on + strand, within RR42_RS10515at 2248.511 kb on + strand, within RR42_RS10515at 2248.751 kb on + strandat 2248.823 kb on + strandat 2248.823 kb on + strandat 2249.163 kb on - strand, within RR42_RS10520at 2249.163 kb on - strand, within RR42_RS10520at 2249.375 kb on + strand, within RR42_RS10520at 2249.408 kb on + strand, within RR42_RS10520at 2249.680 kb on + strandat 2249.681 kb on - strandat 2249.681 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM
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2,247,174 + +0.9
2,247,175 - +0.2
2,247,175 - +0.3
2,247,190 - +0.3
2,247,266 + RR42_RS10510 0.17 +0.1
2,247,358 - RR42_RS10510 0.27 -1.2
2,247,358 - RR42_RS10510 0.27 +0.7
2,247,631 - RR42_RS10510 0.55 +0.7
2,247,632 + RR42_RS10510 0.55 -0.5
2,247,633 - RR42_RS10510 0.55 +1.2
2,248,071 - +0.5
2,248,192 + RR42_RS10515 0.15 +1.5
2,248,373 - RR42_RS10515 0.42 +0.6
2,248,396 + RR42_RS10515 0.45 +0.2
2,248,511 + RR42_RS10515 0.62 +0.3
2,248,751 + -0.1
2,248,823 + -0.2
2,248,823 + +0.0
2,249,163 - RR42_RS10520 0.45 -0.1
2,249,163 - RR42_RS10520 0.45 +0.1
2,249,375 + RR42_RS10520 0.73 +0.7
2,249,408 + RR42_RS10520 0.78 +0.5
2,249,680 + -0.3
2,249,681 - +0.6
2,249,681 - -0.4

Or see this region's nucleotide sequence