Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10485 and RR42_RS10490 are separated by 554 nucleotides RR42_RS10490 and RR42_RS10495 are separated by 401 nucleotides
RR42_RS10485: RR42_RS10485 - hypothetical protein, at 2,242,259 to 2,242,954
_RS10485
RR42_RS10490: RR42_RS10490 - DNA-binding protein, at 2,243,509 to 2,243,826
_RS10490
RR42_RS10495: RR42_RS10495 - PHA-granule associated protein 4, at 2,244,228 to 2,244,602
_RS10495
Position (kb)
2243
2244 Strain fitness (log2 ratio)
-1
0
1 at 2242.713 kb on + strand, within RR42_RS10485 at 2242.713 kb on + strand, within RR42_RS10485 at 2242.714 kb on - strand, within RR42_RS10485 at 2242.714 kb on - strand, within RR42_RS10485 at 2242.714 kb on - strand, within RR42_RS10485 at 2242.740 kb on + strand, within RR42_RS10485 at 2242.769 kb on + strand, within RR42_RS10485 at 2242.770 kb on - strand, within RR42_RS10485 at 2242.849 kb on + strand, within RR42_RS10485 at 2242.849 kb on + strand, within RR42_RS10485 at 2242.849 kb on + strand, within RR42_RS10485 at 2242.948 kb on + strand at 2242.948 kb on + strand at 2242.949 kb on - strand at 2243.002 kb on - strand at 2243.013 kb on + strand at 2243.014 kb on - strand at 2243.014 kb on - strand at 2243.014 kb on - strand at 2243.014 kb on - strand at 2243.022 kb on - strand at 2243.043 kb on + strand at 2243.043 kb on + strand at 2243.264 kb on + strand at 2243.270 kb on + strand at 2243.270 kb on + strand at 2243.271 kb on - strand at 2243.271 kb on - strand at 2243.477 kb on - strand at 2243.607 kb on - strand, within RR42_RS10490 at 2243.702 kb on - strand, within RR42_RS10490 at 2243.708 kb on + strand, within RR42_RS10490 at 2243.708 kb on + strand, within RR42_RS10490 at 2243.867 kb on + strand at 2243.898 kb on + strand at 2243.916 kb on - strand at 2244.165 kb on + strand at 2244.165 kb on + strand at 2244.165 kb on + strand at 2244.175 kb on + strand at 2244.309 kb on + strand, within RR42_RS10495 at 2244.310 kb on - strand, within RR42_RS10495 at 2244.310 kb on - strand, within RR42_RS10495 at 2244.378 kb on + strand, within RR42_RS10495 at 2244.378 kb on + strand, within RR42_RS10495 at 2244.378 kb on + strand, within RR42_RS10495 at 2244.378 kb on + strand, within RR42_RS10495 at 2244.379 kb on - strand, within RR42_RS10495 at 2244.404 kb on + strand, within RR42_RS10495 at 2244.404 kb on + strand, within RR42_RS10495 at 2244.405 kb on - strand, within RR42_RS10495 at 2244.405 kb on - strand, within RR42_RS10495 at 2244.472 kb on - strand, within RR42_RS10495 at 2244.487 kb on + strand, within RR42_RS10495 at 2244.488 kb on - strand, within RR42_RS10495 at 2244.549 kb on + strand, within RR42_RS10495 at 2244.549 kb on + strand, within RR42_RS10495 at 2244.549 kb on + strand, within RR42_RS10495 at 2244.549 kb on + strand, within RR42_RS10495 at 2244.550 kb on - strand, within RR42_RS10495 at 2244.550 kb on - strand, within RR42_RS10495 at 2244.550 kb on - strand, within RR42_RS10495 at 2244.550 kb on - strand, within RR42_RS10495 at 2244.550 kb on - strand, within RR42_RS10495 at 2244.600 kb on + strand at 2244.600 kb on + strand at 2244.600 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 2,242,713 + RR42_RS10485 0.65 +0.6 2,242,713 + RR42_RS10485 0.65 -0.2 2,242,714 - RR42_RS10485 0.65 -1.0 2,242,714 - RR42_RS10485 0.65 +1.1 2,242,714 - RR42_RS10485 0.65 -0.6 2,242,740 + RR42_RS10485 0.69 -0.2 2,242,769 + RR42_RS10485 0.73 +0.6 2,242,770 - RR42_RS10485 0.73 -0.3 2,242,849 + RR42_RS10485 0.85 +0.0 2,242,849 + RR42_RS10485 0.85 -0.3 2,242,849 + RR42_RS10485 0.85 -0.1 2,242,948 + +0.1 2,242,948 + -0.7 2,242,949 - +0.1 2,243,002 - +1.0 2,243,013 + +0.3 2,243,014 - +0.1 2,243,014 - +0.3 2,243,014 - -0.6 2,243,014 - +0.8 2,243,022 - -0.3 2,243,043 + +0.7 2,243,043 + +0.3 2,243,264 + +1.4 2,243,270 + +0.3 2,243,270 + -0.9 2,243,271 - -0.1 2,243,271 - +0.2 2,243,477 - -1.1 2,243,607 - RR42_RS10490 0.31 +1.1 2,243,702 - RR42_RS10490 0.61 +0.7 2,243,708 + RR42_RS10490 0.63 +0.6 2,243,708 + RR42_RS10490 0.63 -0.6 2,243,867 + +0.5 2,243,898 + -0.4 2,243,916 - -0.2 2,244,165 + -0.7 2,244,165 + +0.2 2,244,165 + +0.9 2,244,175 + -0.3 2,244,309 + RR42_RS10495 0.22 +0.3 2,244,310 - RR42_RS10495 0.22 -0.7 2,244,310 - RR42_RS10495 0.22 -0.0 2,244,378 + RR42_RS10495 0.40 +0.0 2,244,378 + RR42_RS10495 0.40 -0.2 2,244,378 + RR42_RS10495 0.40 -0.6 2,244,378 + RR42_RS10495 0.40 -0.3 2,244,379 - RR42_RS10495 0.40 +0.3 2,244,404 + RR42_RS10495 0.47 -0.6 2,244,404 + RR42_RS10495 0.47 -0.0 2,244,405 - RR42_RS10495 0.47 -1.0 2,244,405 - RR42_RS10495 0.47 +0.7 2,244,472 - RR42_RS10495 0.65 -0.5 2,244,487 + RR42_RS10495 0.69 +0.9 2,244,488 - RR42_RS10495 0.69 -0.5 2,244,549 + RR42_RS10495 0.86 -1.0 2,244,549 + RR42_RS10495 0.86 +0.6 2,244,549 + RR42_RS10495 0.86 -1.3 2,244,549 + RR42_RS10495 0.86 -0.7 2,244,550 - RR42_RS10495 0.86 +0.0 2,244,550 - RR42_RS10495 0.86 -0.5 2,244,550 - RR42_RS10495 0.86 +0.3 2,244,550 - RR42_RS10495 0.86 +1.3 2,244,550 - RR42_RS10495 0.86 -0.4 2,244,600 + -0.3 2,244,600 + -0.9 2,244,600 + -0.2
Or see this region's nucleotide sequence