Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09195 and RR42_RS09200 are separated by 602 nucleotides RR42_RS09200 and RR42_RS09205 are separated by 3 nucleotides RR42_RS09205 and RR42_RS09210 are separated by 15 nucleotides
RR42_RS09195: RR42_RS09195 - short-chain dehydrogenase, at 1,943,547 to 1,944,761
_RS09195
RR42_RS09200: RR42_RS09200 - membrane protein, at 1,945,364 to 1,946,965
_RS09200
RR42_RS09205: RR42_RS09205 - cytochrome C oxidase, at 1,946,969 to 1,947,571
_RS09205
RR42_RS09210: RR42_RS09210 - cbb3-type cytochrome C oxidase subunit III, at 1,947,587 to 1,948,195
_RS09210
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1944.610 kb on + strand, within RR42_RS09195 at 1944.611 kb on - strand, within RR42_RS09195 at 1944.611 kb on - strand, within RR42_RS09195 at 1944.611 kb on - strand, within RR42_RS09195 at 1944.664 kb on + strand at 1944.664 kb on + strand at 1944.664 kb on + strand at 1944.664 kb on + strand at 1944.665 kb on - strand at 1944.665 kb on - strand at 1944.665 kb on - strand at 1944.707 kb on - strand at 1945.063 kb on + strand at 1945.066 kb on + strand at 1945.067 kb on - strand at 1945.087 kb on + strand at 1945.113 kb on - strand at 1945.192 kb on + strand at 1945.192 kb on + strand at 1945.193 kb on - strand at 1945.193 kb on - strand at 1945.387 kb on - strand at 1945.559 kb on + strand, within RR42_RS09200 at 1945.844 kb on + strand, within RR42_RS09200 at 1945.844 kb on + strand, within RR42_RS09200 at 1945.844 kb on + strand, within RR42_RS09200 at 1945.845 kb on - strand, within RR42_RS09200 at 1945.845 kb on - strand, within RR42_RS09200 at 1945.852 kb on - strand, within RR42_RS09200 at 1946.036 kb on + strand, within RR42_RS09200 at 1946.037 kb on - strand, within RR42_RS09200 at 1946.037 kb on - strand, within RR42_RS09200 at 1946.037 kb on - strand, within RR42_RS09200 at 1946.177 kb on + strand, within RR42_RS09200 at 1946.178 kb on - strand, within RR42_RS09200 at 1946.205 kb on - strand, within RR42_RS09200 at 1946.207 kb on + strand, within RR42_RS09200 at 1946.288 kb on + strand, within RR42_RS09200 at 1946.288 kb on + strand, within RR42_RS09200 at 1946.289 kb on - strand, within RR42_RS09200 at 1946.344 kb on + strand, within RR42_RS09200 at 1946.345 kb on - strand, within RR42_RS09200 at 1946.558 kb on + strand, within RR42_RS09200 at 1946.559 kb on - strand, within RR42_RS09200 at 1946.624 kb on + strand, within RR42_RS09200 at 1946.624 kb on + strand, within RR42_RS09200 at 1946.678 kb on + strand, within RR42_RS09200 at 1946.717 kb on + strand, within RR42_RS09200 at 1946.756 kb on + strand, within RR42_RS09200 at 1946.814 kb on - strand at 1947.125 kb on + strand, within RR42_RS09205 at 1947.125 kb on + strand, within RR42_RS09205 at 1947.126 kb on - strand, within RR42_RS09205 at 1947.126 kb on - strand, within RR42_RS09205 at 1947.126 kb on - strand, within RR42_RS09205 at 1947.127 kb on + strand, within RR42_RS09205 at 1947.147 kb on - strand, within RR42_RS09205 at 1947.230 kb on + strand, within RR42_RS09205 at 1947.231 kb on - strand, within RR42_RS09205 at 1947.234 kb on - strand, within RR42_RS09205 at 1947.236 kb on + strand, within RR42_RS09205 at 1947.236 kb on + strand, within RR42_RS09205 at 1947.236 kb on + strand, within RR42_RS09205 at 1947.237 kb on - strand, within RR42_RS09205 at 1947.237 kb on - strand, within RR42_RS09205 at 1947.378 kb on - strand, within RR42_RS09205 at 1947.378 kb on - strand, within RR42_RS09205 at 1947.503 kb on + strand, within RR42_RS09205 at 1947.706 kb on - strand, within RR42_RS09210 at 1947.722 kb on + strand, within RR42_RS09210 at 1947.723 kb on - strand, within RR42_RS09210 at 1947.723 kb on - strand, within RR42_RS09210 at 1947.723 kb on - strand, within RR42_RS09210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,944,610 + RR42_RS09195 0.87 +1.1 1,944,611 - RR42_RS09195 0.88 -0.3 1,944,611 - RR42_RS09195 0.88 -0.2 1,944,611 - RR42_RS09195 0.88 -0.4 1,944,664 + +0.9 1,944,664 + -1.3 1,944,664 + -0.3 1,944,664 + +0.5 1,944,665 - -0.7 1,944,665 - -1.5 1,944,665 - +0.4 1,944,707 - -0.3 1,945,063 + -0.4 1,945,066 + -0.4 1,945,067 - -0.4 1,945,087 + +1.4 1,945,113 - +0.4 1,945,192 + -0.5 1,945,192 + -0.1 1,945,193 - -0.8 1,945,193 - -1.5 1,945,387 - +0.4 1,945,559 + RR42_RS09200 0.12 -1.4 1,945,844 + RR42_RS09200 0.30 +1.6 1,945,844 + RR42_RS09200 0.30 -1.5 1,945,844 + RR42_RS09200 0.30 +0.3 1,945,845 - RR42_RS09200 0.30 +0.3 1,945,845 - RR42_RS09200 0.30 +0.4 1,945,852 - RR42_RS09200 0.30 -0.3 1,946,036 + RR42_RS09200 0.42 -0.1 1,946,037 - RR42_RS09200 0.42 +0.2 1,946,037 - RR42_RS09200 0.42 +1.7 1,946,037 - RR42_RS09200 0.42 -0.0 1,946,177 + RR42_RS09200 0.51 -0.4 1,946,178 - RR42_RS09200 0.51 +0.1 1,946,205 - RR42_RS09200 0.52 +0.6 1,946,207 + RR42_RS09200 0.53 +1.4 1,946,288 + RR42_RS09200 0.58 +0.6 1,946,288 + RR42_RS09200 0.58 -1.0 1,946,289 - RR42_RS09200 0.58 +1.7 1,946,344 + RR42_RS09200 0.61 +0.2 1,946,345 - RR42_RS09200 0.61 +1.0 1,946,558 + RR42_RS09200 0.75 +1.1 1,946,559 - RR42_RS09200 0.75 +0.5 1,946,624 + RR42_RS09200 0.79 -0.7 1,946,624 + RR42_RS09200 0.79 -0.2 1,946,678 + RR42_RS09200 0.82 +0.2 1,946,717 + RR42_RS09200 0.84 -0.1 1,946,756 + RR42_RS09200 0.87 -1.0 1,946,814 - +0.8 1,947,125 + RR42_RS09205 0.26 -0.1 1,947,125 + RR42_RS09205 0.26 -0.3 1,947,126 - RR42_RS09205 0.26 -1.3 1,947,126 - RR42_RS09205 0.26 +0.5 1,947,126 - RR42_RS09205 0.26 -1.0 1,947,127 + RR42_RS09205 0.26 +0.8 1,947,147 - RR42_RS09205 0.30 +0.1 1,947,230 + RR42_RS09205 0.43 +0.7 1,947,231 - RR42_RS09205 0.43 +0.3 1,947,234 - RR42_RS09205 0.44 -0.4 1,947,236 + RR42_RS09205 0.44 -0.7 1,947,236 + RR42_RS09205 0.44 +1.2 1,947,236 + RR42_RS09205 0.44 +0.7 1,947,237 - RR42_RS09205 0.44 +0.9 1,947,237 - RR42_RS09205 0.44 -0.1 1,947,378 - RR42_RS09205 0.68 +0.1 1,947,378 - RR42_RS09205 0.68 +1.9 1,947,503 + RR42_RS09205 0.89 +0.7 1,947,706 - RR42_RS09210 0.20 -0.1 1,947,722 + RR42_RS09210 0.22 +0.9 1,947,723 - RR42_RS09210 0.22 +0.4 1,947,723 - RR42_RS09210 0.22 -2.2 1,947,723 - RR42_RS09210 0.22 +0.8
Or see this region's nucleotide sequence