Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08460 and RR42_RS08465 are separated by 61 nucleotides RR42_RS08465 and RR42_RS08470 are separated by 212 nucleotides RR42_RS08470 and RR42_RS08475 are separated by 20 nucleotides RR42_RS08475 and RR42_RS08480 overlap by 8 nucleotides RR42_RS08480 and RR42_RS08485 are separated by 405 nucleotides
RR42_RS08460: RR42_RS08460 - integrase, at 1,782,992 to 1,783,996
_RS08460
RR42_RS08465: RR42_RS08465 - hypothetical protein, at 1,784,058 to 1,784,357
_RS08465
RR42_RS08470: RR42_RS08470 - hypothetical protein, at 1,784,570 to 1,784,776
_RS08470
RR42_RS08475: RR42_RS08475 - hypothetical protein, at 1,784,797 to 1,785,000
_RS08475
RR42_RS08480: RR42_RS08480 - hypothetical protein, at 1,784,993 to 1,785,235
_RS08480
RR42_RS08485: RR42_RS08485 - toprim domain protein, at 1,785,641 to 1,788,346
_RS08485
Position (kb)
1784
1785
1786 Strain fitness (log2 ratio)
-1
0
1 at 1783.855 kb on + strand, within RR42_RS08460 at 1783.927 kb on + strand at 1783.952 kb on + strand at 1783.955 kb on + strand at 1783.956 kb on - strand at 1783.956 kb on - strand at 1783.956 kb on - strand at 1783.964 kb on - strand at 1784.010 kb on + strand at 1784.010 kb on + strand at 1784.036 kb on + strand at 1784.059 kb on + strand at 1784.059 kb on + strand at 1784.060 kb on - strand at 1784.248 kb on + strand, within RR42_RS08465 at 1784.249 kb on - strand, within RR42_RS08465 at 1784.427 kb on + strand at 1784.427 kb on + strand at 1784.428 kb on - strand at 1784.428 kb on - strand at 1784.518 kb on - strand at 1784.561 kb on + strand at 1784.561 kb on + strand at 1784.562 kb on - strand at 1784.562 kb on - strand at 1784.562 kb on - strand at 1784.570 kb on - strand at 1784.698 kb on - strand, within RR42_RS08470 at 1784.796 kb on - strand at 1784.833 kb on - strand, within RR42_RS08475 at 1785.045 kb on + strand, within RR42_RS08480 at 1785.109 kb on - strand, within RR42_RS08480 at 1785.191 kb on + strand, within RR42_RS08480 at 1785.287 kb on - strand at 1785.374 kb on - strand at 1785.374 kb on - strand at 1785.551 kb on + strand at 1785.569 kb on - strand at 1785.638 kb on + strand at 1785.638 kb on + strand at 1785.652 kb on + strand at 1785.948 kb on + strand, within RR42_RS08485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,783,855 + RR42_RS08460 0.86 +0.8 1,783,927 + -1.4 1,783,952 + -0.7 1,783,955 + -0.5 1,783,956 - +0.2 1,783,956 - +0.1 1,783,956 - +0.2 1,783,964 - +0.2 1,784,010 + +0.1 1,784,010 + +0.1 1,784,036 + -0.1 1,784,059 + -1.0 1,784,059 + +0.3 1,784,060 - -0.2 1,784,248 + RR42_RS08465 0.63 -0.1 1,784,249 - RR42_RS08465 0.64 -0.6 1,784,427 + -0.3 1,784,427 + +0.4 1,784,428 - +0.3 1,784,428 - +0.2 1,784,518 - -0.9 1,784,561 + -0.6 1,784,561 + -0.4 1,784,562 - +0.6 1,784,562 - +0.2 1,784,562 - -0.1 1,784,570 - +1.1 1,784,698 - RR42_RS08470 0.62 +0.1 1,784,796 - +0.4 1,784,833 - RR42_RS08475 0.18 +1.5 1,785,045 + RR42_RS08480 0.21 -0.2 1,785,109 - RR42_RS08480 0.48 +0.4 1,785,191 + RR42_RS08480 0.81 -0.3 1,785,287 - -1.3 1,785,374 - +0.2 1,785,374 - -0.4 1,785,551 + -0.1 1,785,569 - +0.1 1,785,638 + +0.2 1,785,638 + +0.5 1,785,652 + -0.8 1,785,948 + RR42_RS08485 0.11 +0.3
Or see this region's nucleotide sequence