Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07990 and RR42_RS07995 overlap by 4 nucleotides RR42_RS07995 and RR42_RS08000 are separated by 60 nucleotides RR42_RS08000 and RR42_RS08005 are separated by 0 nucleotides RR42_RS08005 and RR42_RS08010 are separated by 32 nucleotides
RR42_RS07990: RR42_RS07990 - integrase, at 1,717,192 to 1,718,196
_RS07990
RR42_RS07995: RR42_RS07995 - hypothetical protein, at 1,718,193 to 1,718,519
_RS07995
RR42_RS08000: RR42_RS08000 - hypothetical protein, at 1,718,580 to 1,718,795
_RS08000
RR42_RS08005: RR42_RS08005 - hypothetical protein, at 1,718,796 to 1,719,347
_RS08005
RR42_RS08010: RR42_RS08010 - hypothetical protein, at 1,719,380 to 1,719,634
_RS08010
Position (kb) 1718
1719 Strain fitness (log2 ratio) -1
0
1
2 at 1717.203 kb on + strand at 1717.381 kb on + strand, within RR42_RS07990 at 1717.381 kb on + strand, within RR42_RS07990 at 1717.563 kb on - strand, within RR42_RS07990 at 1717.650 kb on - strand, within RR42_RS07990 at 1718.071 kb on - strand, within RR42_RS07990 at 1718.071 kb on - strand, within RR42_RS07990 at 1718.152 kb on + strand at 1718.153 kb on - strand at 1718.155 kb on + strand at 1718.156 kb on - strand at 1718.156 kb on - strand at 1718.156 kb on - strand at 1718.156 kb on - strand at 1718.411 kb on - strand, within RR42_RS07995 at 1718.595 kb on + strand at 1718.626 kb on + strand, within RR42_RS08000 at 1718.808 kb on + strand at 1718.812 kb on + strand at 1718.863 kb on + strand, within RR42_RS08005 at 1718.863 kb on + strand, within RR42_RS08005 at 1718.863 kb on + strand, within RR42_RS08005 at 1719.142 kb on + strand, within RR42_RS08005 at 1719.234 kb on + strand, within RR42_RS08005 at 1719.401 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,717,203 + -0.5 1,717,381 + RR42_RS07990 0.19 +0.5 1,717,381 + RR42_RS07990 0.19 -1.0 1,717,563 - RR42_RS07990 0.37 -0.5 1,717,650 - RR42_RS07990 0.46 -0.7 1,718,071 - RR42_RS07990 0.87 +0.5 1,718,071 - RR42_RS07990 0.87 +0.2 1,718,152 + -1.3 1,718,153 - -0.5 1,718,155 + -0.2 1,718,156 - -1.0 1,718,156 - +0.2 1,718,156 - -0.1 1,718,156 - +1.9 1,718,411 - RR42_RS07995 0.67 +0.6 1,718,595 + -1.1 1,718,626 + RR42_RS08000 0.21 -1.1 1,718,808 + -0.2 1,718,812 + -0.1 1,718,863 + RR42_RS08005 0.12 -0.4 1,718,863 + RR42_RS08005 0.12 +0.2 1,718,863 + RR42_RS08005 0.12 -0.0 1,719,142 + RR42_RS08005 0.63 +1.1 1,719,234 + RR42_RS08005 0.79 -0.0 1,719,401 + +0.6
Or see this region's nucleotide sequence