Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05405 and RR42_RS05410 are separated by 145 nucleotides RR42_RS05410 and RR42_RS05415 overlap by 4 nucleotides RR42_RS05415 and RR42_RS05420 are separated by 193 nucleotides
RR42_RS05405: RR42_RS05405 - serine/threonine protein phosphatase, at 1,194,177 to 1,195,193
_RS05405
RR42_RS05410: RR42_RS05410 - acetyltransferase, at 1,195,339 to 1,196,316
_RS05410
RR42_RS05415: RR42_RS05415 - membrane protein, at 1,196,313 to 1,196,879
_RS05415
RR42_RS05420: RR42_RS05420 - membrane protein, at 1,197,073 to 1,197,852
_RS05420
Position (kb)
1195
1196
1197 Strain fitness (log2 ratio)
-1
0
1
2 at 1194.353 kb on + strand, within RR42_RS05405 at 1194.427 kb on + strand, within RR42_RS05405 at 1194.428 kb on - strand, within RR42_RS05405 at 1194.428 kb on - strand, within RR42_RS05405 at 1194.428 kb on - strand, within RR42_RS05405 at 1194.461 kb on - strand, within RR42_RS05405 at 1194.461 kb on - strand, within RR42_RS05405 at 1194.461 kb on - strand, within RR42_RS05405 at 1194.541 kb on + strand, within RR42_RS05405 at 1194.542 kb on - strand, within RR42_RS05405 at 1194.559 kb on + strand, within RR42_RS05405 at 1194.560 kb on - strand, within RR42_RS05405 at 1194.727 kb on + strand, within RR42_RS05405 at 1194.728 kb on - strand, within RR42_RS05405 at 1194.799 kb on + strand, within RR42_RS05405 at 1194.848 kb on - strand, within RR42_RS05405 at 1194.889 kb on + strand, within RR42_RS05405 at 1194.889 kb on + strand, within RR42_RS05405 at 1195.269 kb on + strand at 1195.269 kb on + strand at 1195.799 kb on + strand, within RR42_RS05410 at 1195.800 kb on - strand, within RR42_RS05410 at 1196.019 kb on - strand, within RR42_RS05410 at 1196.030 kb on + strand, within RR42_RS05410 at 1196.031 kb on - strand, within RR42_RS05410 at 1196.136 kb on + strand, within RR42_RS05410 at 1196.427 kb on + strand, within RR42_RS05415 at 1196.428 kb on - strand, within RR42_RS05415 at 1196.428 kb on - strand, within RR42_RS05415 at 1196.512 kb on + strand, within RR42_RS05415 at 1196.626 kb on + strand, within RR42_RS05415 at 1196.627 kb on - strand, within RR42_RS05415 at 1196.713 kb on + strand, within RR42_RS05415 at 1196.713 kb on + strand, within RR42_RS05415 at 1196.714 kb on - strand, within RR42_RS05415 at 1196.752 kb on - strand, within RR42_RS05415 at 1196.995 kb on + strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1196.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,194,353 + RR42_RS05405 0.17 +0.9 1,194,427 + RR42_RS05405 0.25 -0.5 1,194,428 - RR42_RS05405 0.25 +0.3 1,194,428 - RR42_RS05405 0.25 +0.0 1,194,428 - RR42_RS05405 0.25 -0.8 1,194,461 - RR42_RS05405 0.28 -0.1 1,194,461 - RR42_RS05405 0.28 +0.1 1,194,461 - RR42_RS05405 0.28 -0.7 1,194,541 + RR42_RS05405 0.36 +0.0 1,194,542 - RR42_RS05405 0.36 +0.7 1,194,559 + RR42_RS05405 0.38 -1.0 1,194,560 - RR42_RS05405 0.38 +0.1 1,194,727 + RR42_RS05405 0.54 -1.2 1,194,728 - RR42_RS05405 0.54 +0.0 1,194,799 + RR42_RS05405 0.61 +0.1 1,194,848 - RR42_RS05405 0.66 +0.5 1,194,889 + RR42_RS05405 0.70 +1.1 1,194,889 + RR42_RS05405 0.70 +0.1 1,195,269 + +0.3 1,195,269 + +0.9 1,195,799 + RR42_RS05410 0.47 -0.7 1,195,800 - RR42_RS05410 0.47 -1.1 1,196,019 - RR42_RS05410 0.70 +0.6 1,196,030 + RR42_RS05410 0.71 +1.1 1,196,031 - RR42_RS05410 0.71 -0.3 1,196,136 + RR42_RS05410 0.81 +1.9 1,196,427 + RR42_RS05415 0.20 -0.5 1,196,428 - RR42_RS05415 0.20 -0.5 1,196,428 - RR42_RS05415 0.20 -0.8 1,196,512 + RR42_RS05415 0.35 -1.0 1,196,626 + RR42_RS05415 0.55 +0.6 1,196,627 - RR42_RS05415 0.55 +1.7 1,196,713 + RR42_RS05415 0.71 -1.2 1,196,713 + RR42_RS05415 0.71 +0.0 1,196,714 - RR42_RS05415 0.71 -0.3 1,196,752 - RR42_RS05415 0.77 +0.5 1,196,995 + -0.1 1,196,996 - -1.6 1,196,996 - -0.5 1,196,996 - -0.7
Or see this region's nucleotide sequence