Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05140 and RR42_RS05145 are separated by 35 nucleotides RR42_RS05145 and RR42_RS05150 are separated by 82 nucleotides
RR42_RS05140: RR42_RS05140 - lipase, at 1,133,426 to 1,134,364
_RS05140
RR42_RS05145: RR42_RS05145 - glyoxalase, at 1,134,400 to 1,134,993
_RS05145
RR42_RS05150: RR42_RS05150 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 1,135,076 to 1,136,014
_RS05150
Position (kb) 1134
1135 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1133.428 kb on - strand at 1133.527 kb on + strand, within RR42_RS05140 at 1133.527 kb on + strand, within RR42_RS05140 at 1133.608 kb on - strand, within RR42_RS05140 at 1133.608 kb on - strand, within RR42_RS05140 at 1133.620 kb on - strand, within RR42_RS05140 at 1133.722 kb on - strand at 1133.722 kb on - strand, within RR42_RS05140 at 1133.722 kb on - strand, within RR42_RS05140 at 1133.724 kb on + strand, within RR42_RS05140 at 1133.910 kb on + strand, within RR42_RS05140 at 1133.911 kb on - strand, within RR42_RS05140 at 1134.055 kb on - strand, within RR42_RS05140 at 1134.061 kb on - strand, within RR42_RS05140 at 1134.061 kb on - strand, within RR42_RS05140 at 1134.156 kb on + strand, within RR42_RS05140 at 1134.707 kb on - strand, within RR42_RS05145 at 1134.746 kb on + strand, within RR42_RS05145 at 1134.746 kb on + strand, within RR42_RS05145 at 1134.746 kb on + strand, within RR42_RS05145 at 1134.747 kb on - strand, within RR42_RS05145 at 1134.749 kb on + strand, within RR42_RS05145 at 1134.915 kb on + strand, within RR42_RS05145 at 1134.917 kb on + strand, within RR42_RS05145 at 1134.918 kb on - strand, within RR42_RS05145 at 1134.978 kb on + strand at 1134.983 kb on - strand at 1135.292 kb on - strand, within RR42_RS05150 at 1135.292 kb on - strand, within RR42_RS05150 at 1135.399 kb on + strand, within RR42_RS05150 at 1135.440 kb on + strand, within RR42_RS05150 at 1135.489 kb on + strand, within RR42_RS05150 at 1135.492 kb on - strand, within RR42_RS05150 at 1135.653 kb on + strand, within RR42_RS05150 at 1135.654 kb on - strand, within RR42_RS05150 at 1135.654 kb on - strand, within RR42_RS05150 at 1135.761 kb on + strand, within RR42_RS05150 at 1135.762 kb on - strand, within RR42_RS05150 at 1135.938 kb on + strand at 1135.938 kb on + strand at 1135.938 kb on + strand at 1135.945 kb on + strand at 1135.989 kb on + strand at 1135.989 kb on + strand at 1135.989 kb on + strand at 1135.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,133,428 - +0.8 1,133,527 + RR42_RS05140 0.11 -0.4 1,133,527 + RR42_RS05140 0.11 +2.5 1,133,608 - RR42_RS05140 0.19 +0.0 1,133,608 - RR42_RS05140 0.19 -0.4 1,133,620 - RR42_RS05140 0.21 -0.3 1,133,722 - +2.3 1,133,722 - RR42_RS05140 0.32 +0.1 1,133,722 - RR42_RS05140 0.32 +0.5 1,133,724 + RR42_RS05140 0.32 +1.1 1,133,910 + RR42_RS05140 0.52 -0.1 1,133,911 - RR42_RS05140 0.52 +0.5 1,134,055 - RR42_RS05140 0.67 +0.2 1,134,061 - RR42_RS05140 0.68 +0.7 1,134,061 - RR42_RS05140 0.68 +0.7 1,134,156 + RR42_RS05140 0.78 +1.0 1,134,707 - RR42_RS05145 0.52 -0.2 1,134,746 + RR42_RS05145 0.58 -0.0 1,134,746 + RR42_RS05145 0.58 +0.8 1,134,746 + RR42_RS05145 0.58 +0.8 1,134,747 - RR42_RS05145 0.58 -1.6 1,134,749 + RR42_RS05145 0.59 +1.2 1,134,915 + RR42_RS05145 0.87 +1.0 1,134,917 + RR42_RS05145 0.87 +1.3 1,134,918 - RR42_RS05145 0.87 -0.8 1,134,978 + -0.0 1,134,983 - -0.4 1,135,292 - RR42_RS05150 0.23 +0.5 1,135,292 - RR42_RS05150 0.23 -0.0 1,135,399 + RR42_RS05150 0.34 -0.5 1,135,440 + RR42_RS05150 0.39 -1.6 1,135,489 + RR42_RS05150 0.44 +0.4 1,135,492 - RR42_RS05150 0.44 -1.5 1,135,653 + RR42_RS05150 0.61 +0.9 1,135,654 - RR42_RS05150 0.62 -0.5 1,135,654 - RR42_RS05150 0.62 -1.0 1,135,761 + RR42_RS05150 0.73 +0.1 1,135,762 - RR42_RS05150 0.73 +0.8 1,135,938 + -0.7 1,135,938 + -3.0 1,135,938 + -1.3 1,135,945 + -0.7 1,135,989 + -0.4 1,135,989 + -0.1 1,135,989 + -1.1 1,135,990 - -0.6
Or see this region's nucleotide sequence