Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05100

Experiment: R2A with Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05095 and RR42_RS05100 are separated by 29 nucleotidesRR42_RS05100 and RR42_RS05105 are separated by 2 nucleotidesRR42_RS05105 and RR42_RS05110 are separated by 45 nucleotides RR42_RS05095: RR42_RS05095 - 4-hyroxy-2-oxovalerate aldolase, at 1,124,735 to 1,125,739 _RS05095 RR42_RS05100: RR42_RS05100 - acetaldehyde dehydrogenase, at 1,125,769 to 1,126,734 _RS05100 RR42_RS05105: RR42_RS05105 - 2-keto-4-pentenoate hydratase, at 1,126,737 to 1,127,528 _RS05105 RR42_RS05110: RR42_RS05110 - betaine-aldehyde dehydrogenase, at 1,127,574 to 1,129,043 _RS05110 Position (kb) 1125 1126 1127Strain fitness (log2 ratio) -1 0 1at 1124.883 kb on + strand, within RR42_RS05095at 1125.349 kb on + strand, within RR42_RS05095at 1125.570 kb on + strand, within RR42_RS05095at 1125.570 kb on + strand, within RR42_RS05095at 1125.571 kb on - strand, within RR42_RS05095at 1125.675 kb on + strandat 1125.843 kb on + strandat 1125.878 kb on + strand, within RR42_RS05100at 1126.344 kb on + strand, within RR42_RS05100at 1126.345 kb on - strand, within RR42_RS05100at 1126.345 kb on - strand, within RR42_RS05100at 1126.397 kb on + strand, within RR42_RS05100at 1126.398 kb on - strand, within RR42_RS05100at 1127.608 kb on + strandat 1127.608 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM
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1,124,883 + RR42_RS05095 0.15 -0.2
1,125,349 + RR42_RS05095 0.61 +0.3
1,125,570 + RR42_RS05095 0.83 -1.0
1,125,570 + RR42_RS05095 0.83 -0.9
1,125,571 - RR42_RS05095 0.83 -0.3
1,125,675 + -1.2
1,125,843 + +1.2
1,125,878 + RR42_RS05100 0.11 +0.2
1,126,344 + RR42_RS05100 0.60 +1.0
1,126,345 - RR42_RS05100 0.60 -0.7
1,126,345 - RR42_RS05100 0.60 +0.7
1,126,397 + RR42_RS05100 0.65 +0.5
1,126,398 - RR42_RS05100 0.65 +0.9
1,127,608 + -1.5
1,127,608 + +0.6

Or see this region's nucleotide sequence