Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03405 and RR42_RS03410 are separated by 16 nucleotides RR42_RS03410 and RR42_RS03415 overlap by 7 nucleotides RR42_RS03415 and RR42_RS03420 are separated by 70 nucleotides
RR42_RS03405: RR42_RS03405 - epoxyqueuosine reductase, at 757,589 to 758,809
_RS03405
RR42_RS03410: RR42_RS03410 - ATPase, at 758,826 to 759,338
_RS03410
RR42_RS03415: RR42_RS03415 - N-acetylmuramoyl-L-alanine amidase, at 759,332 to 760,837
_RS03415
RR42_RS03420: RR42_RS03420 - bile acid:sodium symporter, at 760,908 to 761,930
_RS03420
Position (kb) 759
760
761 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 758.349 kb on + strand, within RR42_RS03405 at 758.452 kb on - strand, within RR42_RS03405 at 758.533 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.820 kb on + strand at 758.820 kb on + strand at 758.820 kb on + strand at 759.506 kb on + strand, within RR42_RS03415 at 759.506 kb on + strand, within RR42_RS03415 at 759.507 kb on - strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.745 kb on + strand, within RR42_RS03415 at 759.746 kb on - strand, within RR42_RS03415 at 759.794 kb on + strand, within RR42_RS03415 at 760.268 kb on - strand, within RR42_RS03415 at 760.268 kb on - strand, within RR42_RS03415 at 760.318 kb on - strand, within RR42_RS03415 at 760.793 kb on + strand at 760.793 kb on + strand at 760.794 kb on - strand at 760.794 kb on - strand at 760.836 kb on - strand at 760.999 kb on + strand at 761.000 kb on - strand at 761.227 kb on + strand, within RR42_RS03420 at 761.227 kb on + strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.272 kb on + strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.427 kb on - strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 758,349 + RR42_RS03405 0.62 -0.4 758,452 - RR42_RS03405 0.71 -0.2 758,533 + RR42_RS03405 0.77 +0.0 758,535 + RR42_RS03405 0.77 -0.3 758,535 + RR42_RS03405 0.77 +0.1 758,535 + RR42_RS03405 0.77 -0.6 758,535 + RR42_RS03405 0.77 -0.9 758,535 + RR42_RS03405 0.77 -0.9 758,820 + -0.3 758,820 + -0.2 758,820 + +1.0 759,506 + RR42_RS03415 0.12 -1.2 759,506 + RR42_RS03415 0.12 -0.2 759,507 - RR42_RS03415 0.12 -1.4 759,656 + RR42_RS03415 0.22 -3.3 759,656 + RR42_RS03415 0.22 -3.1 759,657 - RR42_RS03415 0.22 -1.0 759,657 - RR42_RS03415 0.22 -1.1 759,745 + RR42_RS03415 0.27 -2.0 759,746 - RR42_RS03415 0.27 -0.3 759,794 + RR42_RS03415 0.31 -3.1 760,268 - RR42_RS03415 0.62 +0.0 760,268 - RR42_RS03415 0.62 -3.0 760,318 - RR42_RS03415 0.65 -0.4 760,793 + -0.5 760,793 + -1.8 760,794 - -0.7 760,794 - -2.1 760,836 - -0.0 760,999 + -0.3 761,000 - -1.4 761,227 + RR42_RS03420 0.31 -0.7 761,227 + RR42_RS03420 0.31 +1.2 761,228 - RR42_RS03420 0.31 -1.9 761,228 - RR42_RS03420 0.31 -0.5 761,228 - RR42_RS03420 0.31 +0.7 761,272 + RR42_RS03420 0.36 +0.5 761,273 - RR42_RS03420 0.36 -0.2 761,273 - RR42_RS03420 0.36 -0.4 761,426 + RR42_RS03420 0.51 -0.5 761,426 + RR42_RS03420 0.51 -0.1 761,427 - RR42_RS03420 0.51 -0.0 761,590 + RR42_RS03420 0.67 -0.4 761,590 + RR42_RS03420 0.67 +1.2 761,590 + RR42_RS03420 0.67 -0.5 761,591 - RR42_RS03420 0.67 +0.4 761,591 - RR42_RS03420 0.67 +0.2 761,591 - RR42_RS03420 0.67 -1.2 761,591 - RR42_RS03420 0.67 +0.1 761,591 - RR42_RS03420 0.67 -0.0 761,674 + RR42_RS03420 0.75 +0.5 761,674 + RR42_RS03420 0.75 +1.1 761,674 + RR42_RS03420 0.75 -0.3 761,674 + RR42_RS03420 0.75 +0.8 761,674 + RR42_RS03420 0.75 -0.4 761,675 - RR42_RS03420 0.75 -0.1 761,675 - RR42_RS03420 0.75 -0.4 761,675 - RR42_RS03420 0.75 +0.1 761,675 - RR42_RS03420 0.75 -0.3
Or see this region's nucleotide sequence