Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03105 and RR42_RS03110 are separated by 380 nucleotides RR42_RS03110 and RR42_RS03115 are separated by 102 nucleotides
RR42_RS03105: RR42_RS03105 - integral membrane protein, at 686,610 to 687,305
_RS03105
RR42_RS03110: RR42_RS03110 - pilus assembly protein TapA, at 687,686 to 688,144
_RS03110
RR42_RS03115: RR42_RS03115 - ligase, at 688,247 to 690,127
_RS03115
Position (kb) 687
688
689 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 686.826 kb on + strand, within RR42_RS03105 at 686.827 kb on - strand, within RR42_RS03105 at 686.859 kb on + strand, within RR42_RS03105 at 686.859 kb on + strand, within RR42_RS03105 at 686.860 kb on - strand, within RR42_RS03105 at 686.943 kb on - strand, within RR42_RS03105 at 686.943 kb on - strand, within RR42_RS03105 at 687.088 kb on + strand, within RR42_RS03105 at 687.088 kb on + strand, within RR42_RS03105 at 687.088 kb on + strand, within RR42_RS03105 at 687.089 kb on - strand, within RR42_RS03105 at 687.151 kb on - strand, within RR42_RS03105 at 687.151 kb on - strand, within RR42_RS03105 at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.316 kb on - strand at 687.316 kb on - strand at 687.392 kb on + strand at 687.393 kb on - strand at 687.393 kb on - strand at 687.461 kb on - strand at 687.461 kb on - strand at 687.465 kb on + strand at 687.484 kb on + strand at 687.484 kb on + strand at 687.484 kb on + strand at 687.485 kb on - strand at 687.485 kb on - strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.489 kb on - strand at 687.489 kb on - strand at 687.489 kb on - strand at 687.588 kb on + strand at 687.595 kb on + strand at 687.595 kb on + strand at 687.596 kb on - strand at 687.623 kb on + strand at 687.623 kb on + strand at 687.797 kb on + strand, within RR42_RS03110 at 687.797 kb on + strand, within RR42_RS03110 at 687.797 kb on + strand, within RR42_RS03110 at 687.798 kb on - strand, within RR42_RS03110 at 687.838 kb on - strand, within RR42_RS03110 at 687.898 kb on + strand, within RR42_RS03110 at 687.899 kb on - strand, within RR42_RS03110 at 687.941 kb on - strand, within RR42_RS03110 at 688.072 kb on + strand, within RR42_RS03110 at 688.072 kb on + strand, within RR42_RS03110 at 688.073 kb on - strand, within RR42_RS03110 at 688.146 kb on + strand at 688.147 kb on - strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.205 kb on - strand at 688.205 kb on - strand at 688.205 kb on - strand at 688.231 kb on + strand at 688.231 kb on + strand at 688.232 kb on - strand at 688.336 kb on - strand at 688.346 kb on + strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.433 kb on + strand at 688.707 kb on + strand, within RR42_RS03115 at 688.755 kb on - strand, within RR42_RS03115 at 688.755 kb on - strand, within RR42_RS03115 at 688.964 kb on + strand, within RR42_RS03115 at 688.964 kb on + strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 686,826 + RR42_RS03105 0.31 +0.5 686,827 - RR42_RS03105 0.31 -0.2 686,859 + RR42_RS03105 0.36 -0.3 686,859 + RR42_RS03105 0.36 -0.1 686,860 - RR42_RS03105 0.36 +1.2 686,943 - RR42_RS03105 0.48 +1.2 686,943 - RR42_RS03105 0.48 -0.6 687,088 + RR42_RS03105 0.69 -0.3 687,088 + RR42_RS03105 0.69 -0.1 687,088 + RR42_RS03105 0.69 -0.7 687,089 - RR42_RS03105 0.69 +0.5 687,151 - RR42_RS03105 0.78 -0.2 687,151 - RR42_RS03105 0.78 -0.8 687,315 + -0.4 687,315 + +0.6 687,315 + -0.3 687,315 + +0.4 687,315 + +0.2 687,315 + +0.5 687,316 - -0.5 687,316 - -1.3 687,392 + -0.2 687,393 - -0.2 687,393 - -0.1 687,461 - +0.8 687,461 - -0.5 687,465 + +0.2 687,484 + +0.2 687,484 + -0.7 687,484 + +0.2 687,485 - -0.3 687,485 - +0.3 687,488 + -0.6 687,488 + +0.1 687,488 + -0.6 687,488 + +1.9 687,488 + +0.1 687,488 + -0.4 687,488 + -0.0 687,488 + -1.4 687,488 + -0.6 687,488 + +0.5 687,489 - -0.5 687,489 - -0.2 687,489 - +0.6 687,588 + +1.8 687,595 + +0.5 687,595 + -0.3 687,596 - -0.2 687,623 + +0.5 687,623 + +1.3 687,797 + RR42_RS03110 0.24 -0.9 687,797 + RR42_RS03110 0.24 +0.6 687,797 + RR42_RS03110 0.24 +0.8 687,798 - RR42_RS03110 0.24 -0.2 687,838 - RR42_RS03110 0.33 +3.5 687,898 + RR42_RS03110 0.46 +0.5 687,899 - RR42_RS03110 0.46 +1.3 687,941 - RR42_RS03110 0.56 +0.6 688,072 + RR42_RS03110 0.84 +0.2 688,072 + RR42_RS03110 0.84 +0.9 688,073 - RR42_RS03110 0.84 +0.2 688,146 + -0.3 688,147 - -0.1 688,150 + +1.3 688,150 + -0.9 688,150 + -0.7 688,150 + -0.1 688,151 - -0.1 688,151 - -0.5 688,151 - +0.8 688,151 - -0.9 688,151 - -0.7 688,151 - -0.5 688,151 - -0.5 688,151 - -0.1 688,204 + +0.7 688,204 + +0.4 688,204 + -0.1 688,204 + -0.6 688,205 - +0.5 688,205 - +0.9 688,205 - -0.3 688,231 + -0.0 688,231 + +0.2 688,232 - +0.6 688,336 - -0.6 688,346 + -0.7 688,347 - +0.2 688,347 - +0.7 688,347 - -0.9 688,347 - -0.5 688,433 + -0.3 688,707 + RR42_RS03115 0.24 -1.9 688,755 - RR42_RS03115 0.27 -0.2 688,755 - RR42_RS03115 0.27 -0.1 688,964 + RR42_RS03115 0.38 +0.1 688,964 + RR42_RS03115 0.38 -0.4 688,965 - RR42_RS03115 0.38 -0.5 688,965 - RR42_RS03115 0.38 -0.6 688,965 - RR42_RS03115 0.38 -0.7
Or see this region's nucleotide sequence