Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02585 and RR42_RS02590 overlap by 4 nucleotides RR42_RS02590 and RR42_RS02595 are separated by 145 nucleotides RR42_RS02595 and RR42_RS02600 are separated by 130 nucleotides
RR42_RS02585: RR42_RS02585 - amino acid ABC transporter permease, at 580,857 to 581,540
_RS02585
RR42_RS02590: RR42_RS02590 - amino acid ABC transporter ATP-binding protein, at 581,537 to 582,271
_RS02590
RR42_RS02595: RR42_RS02595 - glutamine amidotransferase, at 582,417 to 583,247
_RS02595
RR42_RS02600: RR42_RS02600 - tartrate dehydrogenase, at 583,378 to 584,466
_RS02600
Position (kb) 582
583
584 Strain fitness (log2 ratio) -1
0
1
2 at 581.475 kb on + strand at 581.476 kb on - strand at 581.476 kb on - strand at 581.483 kb on + strand at 581.536 kb on + strand at 581.536 kb on + strand at 581.536 kb on + strand at 581.568 kb on - strand at 581.568 kb on - strand at 581.894 kb on + strand, within RR42_RS02590 at 581.895 kb on - strand, within RR42_RS02590 at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.677 kb on - strand, within RR42_RS02595 at 582.727 kb on + strand, within RR42_RS02595 at 582.727 kb on + strand, within RR42_RS02595 at 582.727 kb on + strand, within RR42_RS02595 at 582.728 kb on - strand, within RR42_RS02595 at 582.728 kb on - strand, within RR42_RS02595 at 582.883 kb on + strand, within RR42_RS02595 at 582.883 kb on + strand, within RR42_RS02595 at 582.883 kb on + strand, within RR42_RS02595 at 582.884 kb on - strand, within RR42_RS02595 at 583.016 kb on + strand, within RR42_RS02595 at 583.286 kb on + strand at 583.287 kb on - strand at 583.387 kb on + strand at 583.388 kb on - strand at 583.388 kb on - strand at 583.522 kb on + strand, within RR42_RS02600 at 583.522 kb on + strand, within RR42_RS02600 at 583.525 kb on + strand, within RR42_RS02600 at 583.525 kb on + strand, within RR42_RS02600 at 583.525 kb on + strand, within RR42_RS02600 at 583.526 kb on - strand, within RR42_RS02600 at 583.579 kb on + strand, within RR42_RS02600 at 583.591 kb on + strand, within RR42_RS02600 at 583.591 kb on + strand, within RR42_RS02600 at 583.591 kb on + strand, within RR42_RS02600 at 583.632 kb on + strand, within RR42_RS02600 at 583.632 kb on + strand, within RR42_RS02600 at 583.632 kb on + strand, within RR42_RS02600 at 583.632 kb on + strand, within RR42_RS02600 at 583.632 kb on + strand, within RR42_RS02600 at 583.632 kb on + strand, within RR42_RS02600 at 583.633 kb on - strand, within RR42_RS02600 at 583.681 kb on + strand, within RR42_RS02600 at 583.681 kb on + strand, within RR42_RS02600 at 583.681 kb on + strand, within RR42_RS02600 at 583.682 kb on - strand, within RR42_RS02600 at 583.777 kb on + strand, within RR42_RS02600 at 583.778 kb on - strand, within RR42_RS02600 at 583.778 kb on - strand, within RR42_RS02600 at 583.782 kb on - strand, within RR42_RS02600 at 583.990 kb on + strand, within RR42_RS02600 at 584.050 kb on + strand, within RR42_RS02600 at 584.051 kb on - strand, within RR42_RS02600 at 584.193 kb on + strand, within RR42_RS02600 at 584.193 kb on + strand, within RR42_RS02600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 581,475 + -0.5 581,476 - +0.9 581,476 - -0.0 581,483 + -1.2 581,536 + -1.1 581,536 + +0.3 581,536 + +0.7 581,568 - +0.7 581,568 - +0.3 581,894 + RR42_RS02590 0.49 +0.5 581,895 - RR42_RS02590 0.49 -1.6 582,361 + -0.4 582,361 + -0.5 582,361 + +0.4 582,361 + -0.4 582,361 + +0.0 582,361 + +1.1 582,362 - +1.4 582,362 - +2.2 582,362 - +1.5 582,362 - -0.3 582,362 - +0.5 582,673 + RR42_RS02595 0.31 +0.2 582,673 + RR42_RS02595 0.31 +2.1 582,673 + RR42_RS02595 0.31 +2.0 582,673 + RR42_RS02595 0.31 -0.2 582,673 + RR42_RS02595 0.31 -0.5 582,674 - RR42_RS02595 0.31 -0.4 582,674 - RR42_RS02595 0.31 +1.0 582,674 - RR42_RS02595 0.31 +0.6 582,674 - RR42_RS02595 0.31 -0.8 582,677 - RR42_RS02595 0.31 -0.4 582,727 + RR42_RS02595 0.37 +0.6 582,727 + RR42_RS02595 0.37 -0.1 582,727 + RR42_RS02595 0.37 +0.0 582,728 - RR42_RS02595 0.37 +0.3 582,728 - RR42_RS02595 0.37 +0.4 582,883 + RR42_RS02595 0.56 +0.3 582,883 + RR42_RS02595 0.56 +0.4 582,883 + RR42_RS02595 0.56 +0.4 582,884 - RR42_RS02595 0.56 -0.6 583,016 + RR42_RS02595 0.72 -0.4 583,286 + +0.3 583,287 - -0.2 583,387 + +0.6 583,388 - -0.6 583,388 - -0.7 583,522 + RR42_RS02600 0.13 -0.4 583,522 + RR42_RS02600 0.13 -0.9 583,525 + RR42_RS02600 0.13 +1.0 583,525 + RR42_RS02600 0.13 -0.8 583,525 + RR42_RS02600 0.13 +0.1 583,526 - RR42_RS02600 0.14 +0.3 583,579 + RR42_RS02600 0.18 -0.4 583,591 + RR42_RS02600 0.20 +0.7 583,591 + RR42_RS02600 0.20 +1.8 583,591 + RR42_RS02600 0.20 -0.2 583,632 + RR42_RS02600 0.23 +0.6 583,632 + RR42_RS02600 0.23 +1.1 583,632 + RR42_RS02600 0.23 +0.4 583,632 + RR42_RS02600 0.23 -0.6 583,632 + RR42_RS02600 0.23 +1.0 583,632 + RR42_RS02600 0.23 -0.6 583,633 - RR42_RS02600 0.23 -0.5 583,681 + RR42_RS02600 0.28 -0.8 583,681 + RR42_RS02600 0.28 +0.6 583,681 + RR42_RS02600 0.28 +0.4 583,682 - RR42_RS02600 0.28 +0.8 583,777 + RR42_RS02600 0.37 +0.3 583,778 - RR42_RS02600 0.37 +0.5 583,778 - RR42_RS02600 0.37 -0.4 583,782 - RR42_RS02600 0.37 +0.2 583,990 + RR42_RS02600 0.56 +1.3 584,050 + RR42_RS02600 0.62 +0.3 584,051 - RR42_RS02600 0.62 -0.4 584,193 + RR42_RS02600 0.75 +0.7 584,193 + RR42_RS02600 0.75 -0.2
Or see this region's nucleotide sequence