Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01780 and RR42_RS01785 are separated by 52 nucleotides RR42_RS01785 and RR42_RS01790 overlap by 32 nucleotides RR42_RS01790 and RR42_RS01795 are separated by 139 nucleotides
RR42_RS01780: RR42_RS01780 - glutathione synthetase, at 405,076 to 406,029
_RS01780
RR42_RS01785: RR42_RS01785 - PTS fructose transporter subunit IIA, at 406,082 to 406,537
_RS01785
RR42_RS01790: RR42_RS01790 - phosphocarrier protein HPr, at 406,506 to 406,775
_RS01790
RR42_RS01795: RR42_RS01795 - phosphoenolpyruvate-protein phosphotransferase, at 406,915 to 408,690
_RS01795
Position (kb) 406
407 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 405.122 kb on - strand at 405.136 kb on + strand at 405.136 kb on + strand at 405.355 kb on + strand, within RR42_RS01780 at 405.356 kb on - strand, within RR42_RS01780 at 405.356 kb on - strand, within RR42_RS01780 at 405.472 kb on + strand, within RR42_RS01780 at 405.472 kb on + strand, within RR42_RS01780 at 405.473 kb on - strand, within RR42_RS01780 at 405.511 kb on - strand, within RR42_RS01780 at 405.511 kb on - strand, within RR42_RS01780 at 405.670 kb on + strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.672 kb on + strand, within RR42_RS01780 at 405.673 kb on - strand, within RR42_RS01780 at 405.673 kb on - strand, within RR42_RS01780 at 405.733 kb on + strand, within RR42_RS01780 at 405.748 kb on - strand, within RR42_RS01780 at 405.904 kb on + strand, within RR42_RS01780 at 405.905 kb on - strand, within RR42_RS01780 at 406.010 kb on + strand at 406.011 kb on - strand at 406.050 kb on + strand at 406.051 kb on - strand at 406.051 kb on - strand at 406.051 kb on - strand at 406.459 kb on - strand, within RR42_RS01785 at 406.459 kb on - strand, within RR42_RS01785 at 406.459 kb on - strand, within RR42_RS01785 at 406.459 kb on - strand, within RR42_RS01785 at 406.505 kb on + strand at 406.505 kb on + strand at 406.505 kb on + strand at 406.505 kb on + strand at 406.505 kb on + strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.535 kb on + strand at 406.535 kb on + strand at 406.535 kb on + strand at 406.536 kb on - strand at 406.536 kb on - strand at 406.773 kb on + strand at 406.773 kb on + strand at 406.774 kb on - strand at 406.774 kb on - strand at 406.774 kb on - strand at 406.786 kb on - strand at 406.912 kb on + strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 407.008 kb on + strand at 407.008 kb on + strand at 407.008 kb on + strand at 407.008 kb on + strand at 407.009 kb on - strand at 407.009 kb on - strand at 407.009 kb on - strand at 407.089 kb on - strand at 407.089 kb on - strand at 407.215 kb on + strand, within RR42_RS01795 at 407.215 kb on + strand, within RR42_RS01795 at 407.215 kb on + strand, within RR42_RS01795 at 407.216 kb on - strand, within RR42_RS01795 at 407.287 kb on + strand, within RR42_RS01795 at 407.287 kb on + strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.360 kb on + strand, within RR42_RS01795 at 407.360 kb on + strand, within RR42_RS01795 at 407.523 kb on + strand, within RR42_RS01795
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 405,122 - -3.1 405,136 + -0.5 405,136 + -1.5 405,355 + RR42_RS01780 0.29 -2.7 405,356 - RR42_RS01780 0.29 -2.9 405,356 - RR42_RS01780 0.29 -2.3 405,472 + RR42_RS01780 0.42 -2.1 405,472 + RR42_RS01780 0.42 -1.1 405,473 - RR42_RS01780 0.42 -2.8 405,511 - RR42_RS01780 0.46 -2.4 405,511 - RR42_RS01780 0.46 -1.6 405,670 + RR42_RS01780 0.62 -3.4 405,671 - RR42_RS01780 0.62 -0.4 405,671 - RR42_RS01780 0.62 -2.1 405,671 - RR42_RS01780 0.62 -2.6 405,671 - RR42_RS01780 0.62 -1.4 405,672 + RR42_RS01780 0.62 -2.9 405,673 - RR42_RS01780 0.63 -3.9 405,673 - RR42_RS01780 0.63 -2.1 405,733 + RR42_RS01780 0.69 -0.7 405,748 - RR42_RS01780 0.70 -3.3 405,904 + RR42_RS01780 0.87 -3.2 405,905 - RR42_RS01780 0.87 -1.9 406,010 + -0.1 406,011 - -0.7 406,050 + -0.0 406,051 - +0.1 406,051 - +0.8 406,051 - +0.4 406,459 - RR42_RS01785 0.83 +0.5 406,459 - RR42_RS01785 0.83 +1.9 406,459 - RR42_RS01785 0.83 +1.5 406,459 - RR42_RS01785 0.83 +1.8 406,505 + +0.9 406,505 + +0.2 406,505 + -0.1 406,505 + -1.4 406,505 + -1.2 406,506 - +0.4 406,506 - +1.0 406,506 - +0.7 406,506 - -0.5 406,506 - -0.3 406,506 - +0.6 406,506 - +0.7 406,506 - +0.4 406,506 - +1.3 406,506 - +1.3 406,506 - +0.9 406,506 - -1.6 406,506 - +0.7 406,506 - +1.2 406,506 - +0.5 406,528 + +1.4 406,528 + -0.0 406,528 + +0.8 406,528 + +0.5 406,528 + -0.7 406,529 - +2.0 406,529 - +1.5 406,529 - +2.0 406,529 - +0.3 406,529 - +1.0 406,529 - +0.1 406,529 - +1.5 406,529 - +1.0 406,529 - +0.2 406,529 - +0.5 406,529 - +2.4 406,529 - +1.8 406,535 + +2.1 406,535 + +1.6 406,535 + +1.8 406,536 - +1.3 406,536 - +1.8 406,773 + +0.1 406,773 + +0.4 406,774 - +1.5 406,774 - +0.6 406,774 - +0.9 406,786 - +1.0 406,912 + +1.6 406,913 - +0.5 406,913 - +0.9 406,913 - +0.4 406,913 - +1.1 406,913 - -0.0 406,913 - +0.4 406,913 - +0.7 406,913 - +0.2 407,008 + -0.4 407,008 + -0.6 407,008 + +0.5 407,008 + +0.9 407,009 - +0.5 407,009 - +1.5 407,009 - +0.9 407,089 - +0.7 407,089 - +0.4 407,215 + RR42_RS01795 0.17 +0.6 407,215 + RR42_RS01795 0.17 +0.5 407,215 + RR42_RS01795 0.17 +1.2 407,216 - RR42_RS01795 0.17 +0.5 407,287 + RR42_RS01795 0.21 +0.8 407,287 + RR42_RS01795 0.21 +0.2 407,288 - RR42_RS01795 0.21 +0.8 407,288 - RR42_RS01795 0.21 +0.6 407,288 - RR42_RS01795 0.21 +2.3 407,288 - RR42_RS01795 0.21 -0.7 407,288 - RR42_RS01795 0.21 -0.8 407,288 - RR42_RS01795 0.21 +1.1 407,288 - RR42_RS01795 0.21 -0.1 407,360 + RR42_RS01795 0.25 +0.7 407,360 + RR42_RS01795 0.25 -0.3 407,523 + RR42_RS01795 0.34 +0.2
Or see this region's nucleotide sequence