Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01625 and RR42_RS01630 overlap by 4 nucleotides RR42_RS01630 and RR42_RS01635 are separated by 24 nucleotides RR42_RS01635 and RR42_RS01640 are separated by 26 nucleotides
RR42_RS01625: RR42_RS01625 - long-chain fatty acid--CoA ligase, at 369,975 to 371,945
_RS01625
RR42_RS01630: RR42_RS01630 - branched-chain amino acid ABC transporter ATP-binding protein, at 371,942 to 372,865
_RS01630
RR42_RS01635: RR42_RS01635 - ABC transporter permease, at 372,890 to 373,804
_RS01635
RR42_RS01640: RR42_RS01640 - ABC transporter permease, at 373,831 to 374,907
_RS01640
Position (kb) 371
372
373 Strain fitness (log2 ratio) -1
0
1
2 at 371.149 kb on - strand, within RR42_RS01625 at 371.149 kb on - strand, within RR42_RS01625 at 371.274 kb on + strand, within RR42_RS01625 at 371.275 kb on - strand, within RR42_RS01625 at 371.277 kb on + strand, within RR42_RS01625 at 371.277 kb on + strand, within RR42_RS01625 at 371.322 kb on + strand, within RR42_RS01625 at 371.433 kb on + strand, within RR42_RS01625 at 371.464 kb on - strand, within RR42_RS01625 at 371.465 kb on + strand, within RR42_RS01625 at 371.571 kb on + strand, within RR42_RS01625 at 371.572 kb on - strand, within RR42_RS01625 at 371.580 kb on + strand, within RR42_RS01625 at 371.580 kb on + strand, within RR42_RS01625 at 371.766 kb on + strand at 371.767 kb on - strand at 371.767 kb on - strand at 371.767 kb on - strand at 371.768 kb on + strand at 371.768 kb on + strand at 371.805 kb on + strand at 371.900 kb on - strand at 371.900 kb on - strand at 371.900 kb on - strand at 372.163 kb on + strand, within RR42_RS01630 at 372.258 kb on - strand, within RR42_RS01630 at 372.924 kb on + strand at 372.974 kb on + strand at 372.975 kb on - strand at 373.019 kb on + strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.460 kb on - strand, within RR42_RS01635 at 373.460 kb on - strand, within RR42_RS01635 at 373.597 kb on + strand, within RR42_RS01635 at 373.664 kb on + strand, within RR42_RS01635 at 373.665 kb on - strand, within RR42_RS01635 at 373.665 kb on - strand, within RR42_RS01635 at 373.665 kb on - strand, within RR42_RS01635 at 373.731 kb on - strand at 373.731 kb on - strand at 373.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 371,149 - RR42_RS01625 0.60 +1.1 371,149 - RR42_RS01625 0.60 +1.2 371,274 + RR42_RS01625 0.66 +0.4 371,275 - RR42_RS01625 0.66 -0.3 371,277 + RR42_RS01625 0.66 +1.7 371,277 + RR42_RS01625 0.66 -0.0 371,322 + RR42_RS01625 0.68 -0.3 371,433 + RR42_RS01625 0.74 -0.3 371,464 - RR42_RS01625 0.76 +1.8 371,465 + RR42_RS01625 0.76 -1.7 371,571 + RR42_RS01625 0.81 +0.2 371,572 - RR42_RS01625 0.81 -0.5 371,580 + RR42_RS01625 0.81 +0.7 371,580 + RR42_RS01625 0.81 -1.0 371,766 + +0.5 371,767 - +2.2 371,767 - +1.2 371,767 - +0.2 371,768 + +0.4 371,768 + +0.8 371,805 + +0.8 371,900 - -0.2 371,900 - +0.7 371,900 - -0.5 372,163 + RR42_RS01630 0.24 +1.1 372,258 - RR42_RS01630 0.34 -1.2 372,924 + +0.3 372,974 + -0.3 372,975 - -1.7 373,019 + RR42_RS01635 0.14 +1.9 373,020 - RR42_RS01635 0.14 +0.0 373,020 - RR42_RS01635 0.14 +0.9 373,020 - RR42_RS01635 0.14 -1.3 373,020 - RR42_RS01635 0.14 +0.2 373,460 - RR42_RS01635 0.62 +0.2 373,460 - RR42_RS01635 0.62 +0.6 373,597 + RR42_RS01635 0.77 -0.3 373,664 + RR42_RS01635 0.85 -0.9 373,665 - RR42_RS01635 0.85 +0.2 373,665 - RR42_RS01635 0.85 -1.5 373,665 - RR42_RS01635 0.85 +0.7 373,731 - +0.1 373,731 - +0.7 373,731 - -0.5
Or see this region's nucleotide sequence