Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01520 and RR42_RS01525 are separated by 157 nucleotides RR42_RS01525 and RR42_RS01530 are separated by 53 nucleotides
RR42_RS01520: RR42_RS01520 - LysR family transcriptional regulator, at 342,348 to 343,268
_RS01520
RR42_RS01525: RR42_RS01525 - LacI family transcriptional regulator, at 343,426 to 344,427
_RS01525
RR42_RS01530: RR42_RS01530 - isopropylmalate isomerase, at 344,481 to 345,899
_RS01530
Position (kb)
343
344
345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 342.558 kb on + strand, within RR42_RS01520 at 342.558 kb on + strand, within RR42_RS01520 at 342.559 kb on - strand, within RR42_RS01520 at 342.631 kb on - strand, within RR42_RS01520 at 342.673 kb on + strand, within RR42_RS01520 at 342.673 kb on + strand, within RR42_RS01520 at 342.673 kb on + strand, within RR42_RS01520 at 342.674 kb on - strand, within RR42_RS01520 at 342.757 kb on + strand, within RR42_RS01520 at 342.758 kb on - strand, within RR42_RS01520 at 342.774 kb on + strand, within RR42_RS01520 at 342.774 kb on + strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 343.075 kb on + strand, within RR42_RS01520 at 343.076 kb on - strand, within RR42_RS01520 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.191 kb on + strand, within RR42_RS01525 at 344.463 kb on + strand at 344.463 kb on + strand at 344.463 kb on + strand at 344.464 kb on - strand at 344.464 kb on - strand at 344.492 kb on + strand at 344.493 kb on - strand at 344.496 kb on + strand at 344.496 kb on + strand at 344.497 kb on - strand at 344.497 kb on - strand at 344.497 kb on - strand at 344.586 kb on + strand at 344.586 kb on + strand at 344.586 kb on + strand at 344.587 kb on - strand at 344.886 kb on + strand, within RR42_RS01530 at 344.964 kb on + strand, within RR42_RS01530 at 345.045 kb on - strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.211 kb on - strand, within RR42_RS01530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 342,558 + RR42_RS01520 0.23 -1.1 342,558 + RR42_RS01520 0.23 +0.1 342,559 - RR42_RS01520 0.23 +0.5 342,631 - RR42_RS01520 0.31 -0.3 342,673 + RR42_RS01520 0.35 -0.6 342,673 + RR42_RS01520 0.35 +0.3 342,673 + RR42_RS01520 0.35 +0.4 342,674 - RR42_RS01520 0.35 -0.5 342,757 + RR42_RS01520 0.44 -0.3 342,758 - RR42_RS01520 0.45 +0.1 342,774 + RR42_RS01520 0.46 +0.5 342,774 + RR42_RS01520 0.46 +0.3 342,775 - RR42_RS01520 0.46 +0.7 342,775 - RR42_RS01520 0.46 +0.4 342,775 - RR42_RS01520 0.46 +0.5 342,775 - RR42_RS01520 0.46 -0.8 343,075 + RR42_RS01520 0.79 -0.1 343,076 - RR42_RS01520 0.79 -0.4 344,005 + RR42_RS01525 0.58 +0.2 344,005 + RR42_RS01525 0.58 +0.1 344,005 + RR42_RS01525 0.58 -0.2 344,005 + RR42_RS01525 0.58 +1.5 344,005 + RR42_RS01525 0.58 +1.2 344,005 + RR42_RS01525 0.58 -1.5 344,006 - RR42_RS01525 0.58 +1.3 344,006 - RR42_RS01525 0.58 +2.2 344,006 - RR42_RS01525 0.58 -1.1 344,006 - RR42_RS01525 0.58 +0.4 344,006 - RR42_RS01525 0.58 -0.0 344,006 - RR42_RS01525 0.58 +0.5 344,006 - RR42_RS01525 0.58 +0.1 344,191 + RR42_RS01525 0.76 -1.6 344,463 + -2.0 344,463 + -0.8 344,463 + -0.1 344,464 - -0.0 344,464 - -1.7 344,492 + +0.4 344,493 - +2.7 344,496 + -0.1 344,496 + -0.3 344,497 - -0.8 344,497 - -1.2 344,497 - -0.1 344,586 + +0.2 344,586 + +0.0 344,586 + -0.2 344,587 - +0.1 344,886 + RR42_RS01530 0.29 +0.3 344,964 + RR42_RS01530 0.34 +0.3 345,045 - RR42_RS01530 0.40 -0.3 345,210 + RR42_RS01530 0.51 +1.4 345,210 + RR42_RS01530 0.51 -0.5 345,211 - RR42_RS01530 0.51 -0.1
Or see this region's nucleotide sequence