Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01375 and RR42_RS01380 are separated by 129 nucleotides RR42_RS01380 and RR42_RS01385 are separated by 62 nucleotides RR42_RS01385 and RR42_RS01390 are separated by 22 nucleotides
RR42_RS01375: RR42_RS01375 - S-adenosyl-L-homocysteine hydrolase, at 311,482 to 312,915
_RS01375
RR42_RS01380: RR42_RS01380 - membrane protein, at 313,045 to 313,398
_RS01380
RR42_RS01385: RR42_RS01385 - 5,10-methylenetetrahydrofolate reductase, at 313,461 to 314,291
_RS01385
RR42_RS01390: RR42_RS01390 - competence protein, at 314,314 to 314,676
_RS01390
Position (kb) 313
314 Strain fitness (log2 ratio) -1
0
1 at 313.173 kb on + strand, within RR42_RS01380 at 313.173 kb on + strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.340 kb on - strand, within RR42_RS01380 at 313.717 kb on - strand, within RR42_RS01385 at 313.875 kb on + strand, within RR42_RS01385 at 313.962 kb on + strand, within RR42_RS01385 at 314.235 kb on + strand at 314.237 kb on + strand at 314.237 kb on + strand at 314.289 kb on + strand at 314.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 313,173 + RR42_RS01380 0.36 +0.6 313,173 + RR42_RS01380 0.36 +1.6 313,174 - RR42_RS01380 0.36 -0.6 313,174 - RR42_RS01380 0.36 +1.1 313,174 - RR42_RS01380 0.36 -0.3 313,340 - RR42_RS01380 0.83 +0.8 313,717 - RR42_RS01385 0.31 +0.4 313,875 + RR42_RS01385 0.50 +0.3 313,962 + RR42_RS01385 0.60 +0.8 314,235 + +0.3 314,237 + -0.7 314,237 + -0.1 314,289 + +0.6 314,290 - -0.2
Or see this region's nucleotide sequence