Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01160 and RR42_RS01165 overlap by 4 nucleotides RR42_RS01165 and RR42_RS01170 are separated by 40 nucleotides RR42_RS01170 and RR42_RS01175 are separated by 121 nucleotides
RR42_RS01160: RR42_RS01160 - homoserine O-acetyltransferase, at 266,379 to 267,551
_RS01160
RR42_RS01165: RR42_RS01165 - methionine biosynthesis protein MetW, at 267,548 to 268,159
_RS01165
RR42_RS01170: RR42_RS01170 - LysR family transcriptional regulator, at 268,200 to 269,117
_RS01170
RR42_RS01175: RR42_RS01175 - membrane protein, at 269,239 to 270,183
_RS01175
Position (kb) 268
269
270 Strain fitness (log2 ratio) -2
-1
0
1 at 267.231 kb on + strand, within RR42_RS01160 at 267.231 kb on + strand, within RR42_RS01160 at 267.249 kb on + strand, within RR42_RS01160 at 267.276 kb on + strand, within RR42_RS01160 at 267.439 kb on - strand at 267.439 kb on - strand at 267.439 kb on - strand at 267.496 kb on - strand at 267.516 kb on + strand at 267.517 kb on - strand at 267.517 kb on - strand at 267.520 kb on - strand at 267.541 kb on + strand at 267.541 kb on + strand at 267.542 kb on - strand at 267.542 kb on - strand at 267.701 kb on + strand, within RR42_RS01165 at 267.815 kb on - strand, within RR42_RS01165 at 267.984 kb on - strand, within RR42_RS01165 at 267.992 kb on + strand, within RR42_RS01165 at 267.992 kb on + strand, within RR42_RS01165 at 267.993 kb on - strand, within RR42_RS01165 at 268.009 kb on - strand, within RR42_RS01165 at 268.009 kb on - strand, within RR42_RS01165 at 268.237 kb on + strand at 268.238 kb on - strand at 268.245 kb on - strand at 268.285 kb on + strand at 268.286 kb on - strand at 268.305 kb on + strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.540 kb on + strand, within RR42_RS01170 at 268.540 kb on + strand, within RR42_RS01170 at 268.541 kb on - strand, within RR42_RS01170 at 268.541 kb on - strand, within RR42_RS01170 at 269.085 kb on + strand at 269.086 kb on - strand at 269.131 kb on + strand at 269.132 kb on - strand at 269.142 kb on - strand at 269.152 kb on + strand at 269.215 kb on + strand at 269.216 kb on - strand at 269.216 kb on - strand at 269.216 kb on - strand at 269.227 kb on + strand at 269.228 kb on - strand at 269.228 kb on - strand at 269.523 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.567 kb on - strand, within RR42_RS01175 at 269.596 kb on + strand, within RR42_RS01175 at 269.785 kb on + strand, within RR42_RS01175 at 269.956 kb on - strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 270.034 kb on + strand, within RR42_RS01175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 267,231 + RR42_RS01160 0.73 -0.3 267,231 + RR42_RS01160 0.73 +0.5 267,249 + RR42_RS01160 0.74 -2.5 267,276 + RR42_RS01160 0.76 -0.4 267,439 - +0.4 267,439 - +0.3 267,439 - +1.0 267,496 - +0.0 267,516 + -0.7 267,517 - +0.1 267,517 - -0.2 267,520 - -0.4 267,541 + -1.4 267,541 + -1.7 267,542 - -0.0 267,542 - -0.8 267,701 + RR42_RS01165 0.25 +0.4 267,815 - RR42_RS01165 0.44 -0.4 267,984 - RR42_RS01165 0.71 +0.2 267,992 + RR42_RS01165 0.73 -0.6 267,992 + RR42_RS01165 0.73 -1.4 267,993 - RR42_RS01165 0.73 +0.0 268,009 - RR42_RS01165 0.75 +0.2 268,009 - RR42_RS01165 0.75 -2.1 268,237 + -0.2 268,238 - +0.1 268,245 - +0.5 268,285 + +0.3 268,286 - +0.4 268,305 + RR42_RS01170 0.11 +1.2 268,306 - RR42_RS01170 0.12 -0.5 268,306 - RR42_RS01170 0.12 +1.0 268,306 - RR42_RS01170 0.12 +1.0 268,306 - RR42_RS01170 0.12 +0.1 268,540 + RR42_RS01170 0.37 +0.3 268,540 + RR42_RS01170 0.37 +0.1 268,541 - RR42_RS01170 0.37 -1.0 268,541 - RR42_RS01170 0.37 +0.1 269,085 + +0.9 269,086 - +1.1 269,131 + -1.2 269,132 - -0.9 269,142 - -0.6 269,152 + -0.2 269,215 + -0.4 269,216 - +0.0 269,216 - -0.1 269,216 - -0.2 269,227 + +0.4 269,228 - +0.1 269,228 - +0.9 269,523 + RR42_RS01175 0.30 +0.8 269,566 + RR42_RS01175 0.35 -0.2 269,566 + RR42_RS01175 0.35 +0.2 269,566 + RR42_RS01175 0.35 +0.4 269,567 - RR42_RS01175 0.35 +0.3 269,596 + RR42_RS01175 0.38 +0.2 269,785 + RR42_RS01175 0.58 -1.3 269,956 - RR42_RS01175 0.76 -0.8 269,968 + RR42_RS01175 0.77 +0.4 269,968 + RR42_RS01175 0.77 +0.6 269,968 + RR42_RS01175 0.77 -0.7 269,968 + RR42_RS01175 0.77 -1.1 269,969 - RR42_RS01175 0.77 -0.3 269,969 - RR42_RS01175 0.77 +0.2 269,969 - RR42_RS01175 0.77 +0.1 269,969 - RR42_RS01175 0.77 +1.2 269,980 + RR42_RS01175 0.78 +0.2 269,980 + RR42_RS01175 0.78 -0.0 269,980 + RR42_RS01175 0.78 -0.6 269,980 + RR42_RS01175 0.78 -0.3 269,981 - RR42_RS01175 0.79 +0.3 269,981 - RR42_RS01175 0.79 +0.3 269,981 - RR42_RS01175 0.79 -0.6 269,981 - RR42_RS01175 0.79 -0.0 270,034 + RR42_RS01175 0.84 -0.2
Or see this region's nucleotide sequence