Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01100 and RR42_RS01105 are separated by 94 nucleotides RR42_RS01105 and RR42_RS01110 are separated by 0 nucleotides RR42_RS01110 and RR42_RS01115 overlap by 4 nucleotides RR42_RS01115 and RR42_RS01120 are separated by 90 nucleotides RR42_RS01120 and RR42_RS01125 are separated by 294 nucleotides
RR42_RS01100: RR42_RS01100 - LysR family transcriptional regulator, at 256,406 to 257,341
_RS01100
RR42_RS01105: RR42_RS01105 - membrane protein, at 257,436 to 257,948
_RS01105
RR42_RS01110: RR42_RS01110 - hypothetical protein, at 257,949 to 258,176
_RS01110
RR42_RS01115: RR42_RS01115 - hypothetical protein, at 258,173 to 258,400
_RS01115
RR42_RS01120: RR42_RS01120 - RNA polymerase sigma factor, at 258,491 to 259,072
_RS01120
RR42_RS01125: RR42_RS01125 - acetylglutamate kinase, at 259,367 to 260,284
_RS01125
Position (kb) 258
259 Strain fitness (log2 ratio) -1
0
1
2 at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.350 kb on + strand at 257.350 kb on + strand at 257.360 kb on - strand at 257.392 kb on - strand at 257.706 kb on + strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.757 kb on - strand, within RR42_RS01105 at 258.391 kb on + strand at 258.392 kb on - strand at 258.552 kb on + strand, within RR42_RS01120 at 258.553 kb on - strand, within RR42_RS01120 at 258.553 kb on - strand, within RR42_RS01120 at 258.571 kb on + strand, within RR42_RS01120 at 258.571 kb on + strand, within RR42_RS01120 at 258.572 kb on - strand, within RR42_RS01120 at 258.572 kb on - strand, within RR42_RS01120 at 258.599 kb on + strand, within RR42_RS01120 at 258.620 kb on + strand, within RR42_RS01120 at 258.620 kb on + strand, within RR42_RS01120 at 258.748 kb on + strand, within RR42_RS01120 at 258.749 kb on - strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.754 kb on - strand, within RR42_RS01120 at 258.872 kb on - strand, within RR42_RS01120 at 258.872 kb on - strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.907 kb on - strand, within RR42_RS01120 at 258.980 kb on - strand, within RR42_RS01120 at 259.114 kb on + strand at 259.114 kb on + strand at 259.114 kb on + strand at 259.114 kb on + strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.158 kb on + strand at 259.158 kb on + strand at 259.181 kb on + strand at 259.247 kb on - strand at 259.279 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 257,273 - +0.7 257,273 - -1.7 257,273 - -1.7 257,273 - -0.9 257,350 + -0.7 257,350 + +0.5 257,360 - +0.2 257,392 - +0.2 257,706 + RR42_RS01105 0.53 -0.5 257,707 - RR42_RS01105 0.53 +0.4 257,707 - RR42_RS01105 0.53 -0.5 257,707 - RR42_RS01105 0.53 +0.1 257,707 - RR42_RS01105 0.53 -1.1 257,712 - RR42_RS01105 0.54 +1.2 257,712 - RR42_RS01105 0.54 -1.0 257,712 - RR42_RS01105 0.54 -0.2 257,757 - RR42_RS01105 0.63 +0.9 258,391 + -0.8 258,392 - +0.4 258,552 + RR42_RS01120 0.10 -0.2 258,553 - RR42_RS01120 0.11 -0.7 258,553 - RR42_RS01120 0.11 -0.6 258,571 + RR42_RS01120 0.14 -1.4 258,571 + RR42_RS01120 0.14 +0.8 258,572 - RR42_RS01120 0.14 -0.4 258,572 - RR42_RS01120 0.14 +0.0 258,599 + RR42_RS01120 0.19 -0.6 258,620 + RR42_RS01120 0.22 +2.0 258,620 + RR42_RS01120 0.22 -0.0 258,748 + RR42_RS01120 0.44 -0.2 258,749 - RR42_RS01120 0.44 +0.1 258,753 + RR42_RS01120 0.45 +0.4 258,753 + RR42_RS01120 0.45 +1.0 258,753 + RR42_RS01120 0.45 +0.6 258,754 - RR42_RS01120 0.45 +0.3 258,872 - RR42_RS01120 0.65 -0.7 258,872 - RR42_RS01120 0.65 +0.4 258,906 + RR42_RS01120 0.71 +0.0 258,906 + RR42_RS01120 0.71 +1.1 258,906 + RR42_RS01120 0.71 -0.5 258,907 - RR42_RS01120 0.71 -0.1 258,980 - RR42_RS01120 0.84 -0.1 259,114 + -0.8 259,114 + +0.4 259,114 + +0.7 259,114 + -0.1 259,115 - +0.5 259,115 - +0.2 259,115 - +0.2 259,115 - +0.8 259,115 - -0.0 259,158 + +1.0 259,158 + +0.1 259,181 + -0.4 259,247 - -0.3 259,279 + +0.8 259,336 + -1.0 259,336 + +0.9 259,336 + -0.6 259,336 + +0.7 259,336 + -0.2
Or see this region's nucleotide sequence