Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00725 and RR42_RS00730 are separated by 69 nucleotides RR42_RS00730 and RR42_RS00735 are separated by 265 nucleotides
RR42_RS00725: RR42_RS00725 - hypothetical protein, at 183,138 to 184,046
_RS00725
RR42_RS00730: RR42_RS00730 - enoyl-CoA hydratase, at 184,116 to 184,934
_RS00730
RR42_RS00735: RR42_RS00735 - fumarylacetoacetate hydrolase, at 185,200 to 186,183
_RS00735
Position (kb)
184
185 Strain fitness (log2 ratio)
-2
-1
0
1 at 183.159 kb on - strand at 183.450 kb on - strand, within RR42_RS00725 at 183.657 kb on - strand, within RR42_RS00725 at 183.657 kb on - strand, within RR42_RS00725 at 183.706 kb on + strand, within RR42_RS00725 at 183.706 kb on + strand, within RR42_RS00725 at 183.707 kb on - strand, within RR42_RS00725 at 184.108 kb on + strand at 184.108 kb on + strand at 184.109 kb on - strand at 184.109 kb on - strand at 184.109 kb on - strand at 184.109 kb on - strand at 184.155 kb on + strand at 184.156 kb on - strand at 184.244 kb on + strand, within RR42_RS00730 at 184.245 kb on - strand, within RR42_RS00730 at 184.255 kb on + strand, within RR42_RS00730 at 184.255 kb on + strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.487 kb on + strand, within RR42_RS00730 at 184.487 kb on + strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.618 kb on + strand, within RR42_RS00730 at 184.618 kb on + strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.799 kb on + strand, within RR42_RS00730 at 184.799 kb on + strand, within RR42_RS00730 at 184.800 kb on - strand, within RR42_RS00730 at 185.360 kb on + strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.803 kb on - strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 183,159 - +0.1 183,450 - RR42_RS00725 0.34 +0.6 183,657 - RR42_RS00725 0.57 -1.0 183,657 - RR42_RS00725 0.57 -2.4 183,706 + RR42_RS00725 0.62 -1.4 183,706 + RR42_RS00725 0.62 -0.9 183,707 - RR42_RS00725 0.63 +0.8 184,108 + -2.0 184,108 + +0.4 184,109 - -0.1 184,109 - +0.5 184,109 - +0.1 184,109 - -0.7 184,155 + -0.9 184,156 - +0.5 184,244 + RR42_RS00730 0.16 +1.2 184,245 - RR42_RS00730 0.16 +0.9 184,255 + RR42_RS00730 0.17 -1.0 184,255 + RR42_RS00730 0.17 +0.3 184,256 - RR42_RS00730 0.17 -1.0 184,256 - RR42_RS00730 0.17 +0.5 184,256 - RR42_RS00730 0.17 -0.2 184,408 + RR42_RS00730 0.36 -0.5 184,408 + RR42_RS00730 0.36 -1.2 184,408 + RR42_RS00730 0.36 -1.3 184,409 - RR42_RS00730 0.36 +0.5 184,409 - RR42_RS00730 0.36 -0.5 184,409 - RR42_RS00730 0.36 +0.4 184,409 - RR42_RS00730 0.36 +0.2 184,411 + RR42_RS00730 0.36 +1.6 184,411 + RR42_RS00730 0.36 -0.6 184,411 + RR42_RS00730 0.36 +0.3 184,411 + RR42_RS00730 0.36 -0.3 184,411 + RR42_RS00730 0.36 +0.4 184,411 + RR42_RS00730 0.36 +0.8 184,411 + RR42_RS00730 0.36 +1.7 184,411 + RR42_RS00730 0.36 -1.5 184,412 - RR42_RS00730 0.36 +0.8 184,412 - RR42_RS00730 0.36 -0.2 184,412 - RR42_RS00730 0.36 +0.5 184,487 + RR42_RS00730 0.45 -0.1 184,487 + RR42_RS00730 0.45 +0.2 184,530 - RR42_RS00730 0.51 -0.5 184,530 - RR42_RS00730 0.51 -0.3 184,530 - RR42_RS00730 0.51 +0.2 184,618 + RR42_RS00730 0.61 -0.4 184,618 + RR42_RS00730 0.61 -0.5 184,619 - RR42_RS00730 0.61 -1.2 184,619 - RR42_RS00730 0.61 +0.2 184,619 - RR42_RS00730 0.61 -0.9 184,799 + RR42_RS00730 0.83 -1.6 184,799 + RR42_RS00730 0.83 +0.1 184,800 - RR42_RS00730 0.84 +0.0 185,360 + RR42_RS00735 0.16 +0.2 185,440 - RR42_RS00735 0.24 +0.1 185,440 - RR42_RS00735 0.24 -0.7 185,585 - RR42_RS00735 0.39 +0.4 185,585 - RR42_RS00735 0.39 +0.2 185,668 + RR42_RS00735 0.48 +1.2 185,668 + RR42_RS00735 0.48 -1.6 185,668 + RR42_RS00735 0.48 -1.4 185,668 + RR42_RS00735 0.48 +0.2 185,668 + RR42_RS00735 0.48 +0.2 185,669 - RR42_RS00735 0.48 +0.1 185,669 - RR42_RS00735 0.48 +0.8 185,669 - RR42_RS00735 0.48 -0.1 185,669 - RR42_RS00735 0.48 +0.8 185,803 - RR42_RS00735 0.61 +1.1 185,843 + RR42_RS00735 0.65 -1.2 185,843 + RR42_RS00735 0.65 +0.1 185,843 + RR42_RS00735 0.65 +1.4 185,844 - RR42_RS00735 0.65 +0.6 185,844 - RR42_RS00735 0.65 -1.2 185,844 - RR42_RS00735 0.65 -1.2 185,844 - RR42_RS00735 0.65 -0.5
Or see this region's nucleotide sequence