Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00265 and RR42_RS00270 are separated by 57 nucleotides RR42_RS00270 and RR42_RS00275 are separated by 59 nucleotides RR42_RS00275 and RR42_RS00280 are separated by 81 nucleotides RR42_RS00280 and RR42_RS00285 are separated by 269 nucleotides
RR42_RS00265: RR42_RS00265 - phosphate ABC transporter ATP-binding protein, at 78,893 to 79,621
_RS00265
RR42_RS00270: RR42_RS00270 - amino acid ABC transporter, at 79,679 to 80,485
_RS00270
RR42_RS00275: RR42_RS00275 - galactarate dehydratase, at 80,545 to 82,113
_RS00275
RR42_RS00280: RR42_RS00280 - glutathione peroxidase, at 82,195 to 82,746
_RS00280
RR42_RS00285: RR42_RS00285 - thiamine biosynthesis protein ThiS, at 83,016 to 83,285
_RS00285
Position (kb) 80
81
82
83 Strain fitness (log2 ratio) -2
-1
0
1 at 79.661 kb on - strand at 79.682 kb on + strand at 79.682 kb on + strand at 79.682 kb on + strand at 79.683 kb on - strand at 79.683 kb on - strand at 79.683 kb on - strand at 79.687 kb on + strand at 79.687 kb on + strand at 79.688 kb on - strand at 80.199 kb on - strand, within RR42_RS00270 at 80.337 kb on - strand, within RR42_RS00270 at 80.342 kb on + strand, within RR42_RS00270 at 80.342 kb on + strand, within RR42_RS00270 at 80.343 kb on - strand, within RR42_RS00270 at 80.439 kb on - strand at 80.439 kb on - strand at 80.439 kb on - strand at 80.447 kb on + strand at 80.447 kb on + strand at 80.448 kb on - strand at 80.483 kb on + strand at 80.483 kb on + strand at 80.483 kb on + strand at 80.483 kb on + strand at 80.484 kb on - strand at 80.484 kb on - strand at 80.484 kb on - strand at 80.484 kb on - strand at 80.589 kb on + strand at 80.590 kb on - strand at 80.590 kb on - strand at 80.590 kb on - strand at 80.851 kb on + strand, within RR42_RS00275 at 80.941 kb on - strand, within RR42_RS00275 at 80.947 kb on - strand, within RR42_RS00275 at 80.950 kb on + strand, within RR42_RS00275 at 80.950 kb on + strand, within RR42_RS00275 at 80.951 kb on - strand, within RR42_RS00275 at 80.965 kb on - strand, within RR42_RS00275 at 80.965 kb on - strand, within RR42_RS00275 at 80.965 kb on - strand, within RR42_RS00275 at 81.151 kb on + strand, within RR42_RS00275 at 81.518 kb on - strand, within RR42_RS00275 at 81.518 kb on - strand, within RR42_RS00275 at 81.604 kb on + strand, within RR42_RS00275 at 81.605 kb on - strand, within RR42_RS00275 at 81.605 kb on - strand, within RR42_RS00275 at 81.605 kb on - strand, within RR42_RS00275 at 81.605 kb on - strand, within RR42_RS00275 at 81.736 kb on + strand, within RR42_RS00275 at 81.736 kb on + strand, within RR42_RS00275 at 81.736 kb on + strand, within RR42_RS00275 at 81.737 kb on - strand, within RR42_RS00275 at 81.742 kb on + strand, within RR42_RS00275 at 81.743 kb on - strand, within RR42_RS00275 at 81.872 kb on - strand, within RR42_RS00275 at 82.107 kb on + strand at 82.277 kb on - strand, within RR42_RS00280 at 82.425 kb on - strand, within RR42_RS00280 at 82.487 kb on + strand, within RR42_RS00280 at 82.487 kb on + strand, within RR42_RS00280 at 82.487 kb on + strand, within RR42_RS00280 at 82.488 kb on - strand, within RR42_RS00280 at 82.488 kb on - strand, within RR42_RS00280 at 82.488 kb on - strand, within RR42_RS00280 at 82.568 kb on - strand, within RR42_RS00280 at 82.599 kb on - strand, within RR42_RS00280 at 82.619 kb on + strand, within RR42_RS00280 at 82.620 kb on - strand, within RR42_RS00280 at 82.620 kb on - strand, within RR42_RS00280 at 82.730 kb on + strand at 82.731 kb on - strand at 82.731 kb on - strand at 82.791 kb on + strand at 82.881 kb on + strand at 82.881 kb on + strand at 82.882 kb on - strand at 82.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 79,661 - +0.3 79,682 + -0.4 79,682 + +0.3 79,682 + -0.5 79,683 - -0.3 79,683 - +0.5 79,683 - -0.4 79,687 + -0.6 79,687 + +0.3 79,688 - -0.8 80,199 - RR42_RS00270 0.64 -0.2 80,337 - RR42_RS00270 0.82 -0.8 80,342 + RR42_RS00270 0.82 -0.8 80,342 + RR42_RS00270 0.82 -0.1 80,343 - RR42_RS00270 0.82 +1.3 80,439 - -0.6 80,439 - -0.1 80,439 - +0.9 80,447 + -1.0 80,447 + -2.0 80,448 - +0.9 80,483 + -0.6 80,483 + -0.1 80,483 + +0.8 80,483 + +0.1 80,484 - +0.8 80,484 - -0.1 80,484 - -0.6 80,484 - -0.1 80,589 + +0.3 80,590 - -0.3 80,590 - +1.5 80,590 - +1.2 80,851 + RR42_RS00275 0.20 +0.8 80,941 - RR42_RS00275 0.25 +0.4 80,947 - RR42_RS00275 0.26 -0.1 80,950 + RR42_RS00275 0.26 -0.3 80,950 + RR42_RS00275 0.26 -1.0 80,951 - RR42_RS00275 0.26 +0.0 80,965 - RR42_RS00275 0.27 -0.6 80,965 - RR42_RS00275 0.27 +0.4 80,965 - RR42_RS00275 0.27 -1.6 81,151 + RR42_RS00275 0.39 +0.2 81,518 - RR42_RS00275 0.62 +0.7 81,518 - RR42_RS00275 0.62 +0.3 81,604 + RR42_RS00275 0.67 +0.9 81,605 - RR42_RS00275 0.68 +0.0 81,605 - RR42_RS00275 0.68 +0.9 81,605 - RR42_RS00275 0.68 -0.1 81,605 - RR42_RS00275 0.68 -0.3 81,736 + RR42_RS00275 0.76 -0.4 81,736 + RR42_RS00275 0.76 -0.3 81,736 + RR42_RS00275 0.76 +1.0 81,737 - RR42_RS00275 0.76 +1.3 81,742 + RR42_RS00275 0.76 -0.1 81,743 - RR42_RS00275 0.76 +0.4 81,872 - RR42_RS00275 0.85 +0.7 82,107 + +0.9 82,277 - RR42_RS00280 0.15 -2.4 82,425 - RR42_RS00280 0.42 -0.1 82,487 + RR42_RS00280 0.53 -0.6 82,487 + RR42_RS00280 0.53 -0.1 82,487 + RR42_RS00280 0.53 -1.1 82,488 - RR42_RS00280 0.53 +0.9 82,488 - RR42_RS00280 0.53 +0.5 82,488 - RR42_RS00280 0.53 -1.2 82,568 - RR42_RS00280 0.68 -1.3 82,599 - RR42_RS00280 0.73 +0.2 82,619 + RR42_RS00280 0.77 -0.5 82,620 - RR42_RS00280 0.77 +0.9 82,620 - RR42_RS00280 0.77 +0.2 82,730 + -0.4 82,731 - -1.1 82,731 - -0.8 82,791 + +0.5 82,881 + -0.1 82,881 + +0.7 82,882 - +0.1 82,953 + -0.7
Or see this region's nucleotide sequence