Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00245 and RR42_RS00250 are separated by 17 nucleotides RR42_RS00250 and RR42_RS00255 are separated by 271 nucleotides
RR42_RS00245: RR42_RS00245 - microcystin degradation protein MlrC, at 74,621 to 76,102
_RS00245
RR42_RS00250: RR42_RS00250 - GntR family transcriptional regulator, at 76,120 to 77,166
_RS00250
RR42_RS00255: RR42_RS00255 - ABC transporter permease, at 77,438 to 78,121
_RS00255
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-2
-1
0
1 at 75.314 kb on - strand, within RR42_RS00245 at 75.428 kb on + strand, within RR42_RS00245 at 75.768 kb on - strand, within RR42_RS00245 at 75.768 kb on - strand, within RR42_RS00245 at 75.789 kb on - strand, within RR42_RS00245 at 75.896 kb on - strand, within RR42_RS00245 at 75.953 kb on + strand, within RR42_RS00245 at 75.953 kb on + strand, within RR42_RS00245 at 76.100 kb on + strand at 76.101 kb on - strand at 76.130 kb on + strand at 76.130 kb on + strand at 76.131 kb on - strand at 76.131 kb on - strand at 76.199 kb on - strand at 76.461 kb on - strand, within RR42_RS00250 at 76.666 kb on + strand, within RR42_RS00250 at 76.666 kb on + strand, within RR42_RS00250 at 76.667 kb on - strand, within RR42_RS00250 at 76.779 kb on - strand, within RR42_RS00250 at 76.779 kb on - strand, within RR42_RS00250 at 77.006 kb on - strand, within RR42_RS00250 at 77.069 kb on + strand at 77.276 kb on + strand at 77.276 kb on + strand at 77.276 kb on + strand at 77.276 kb on + strand at 77.276 kb on + strand at 77.277 kb on - strand at 77.444 kb on + strand at 77.444 kb on + strand at 77.616 kb on - strand, within RR42_RS00255 at 77.739 kb on + strand, within RR42_RS00255 at 77.750 kb on - strand, within RR42_RS00255 at 77.879 kb on + strand, within RR42_RS00255 at 77.880 kb on - strand, within RR42_RS00255 at 77.880 kb on - strand, within RR42_RS00255 at 78.008 kb on + strand, within RR42_RS00255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 75,314 - RR42_RS00245 0.47 +0.5 75,428 + RR42_RS00245 0.54 -1.8 75,768 - RR42_RS00245 0.77 -0.3 75,768 - RR42_RS00245 0.77 +0.1 75,789 - RR42_RS00245 0.79 -1.0 75,896 - RR42_RS00245 0.86 -0.2 75,953 + RR42_RS00245 0.90 -1.2 75,953 + RR42_RS00245 0.90 -0.5 76,100 + +0.3 76,101 - +0.6 76,130 + -0.5 76,130 + +0.5 76,131 - +1.0 76,131 - +0.3 76,199 - +0.1 76,461 - RR42_RS00250 0.33 -0.5 76,666 + RR42_RS00250 0.52 -1.2 76,666 + RR42_RS00250 0.52 +1.1 76,667 - RR42_RS00250 0.52 -0.6 76,779 - RR42_RS00250 0.63 -1.3 76,779 - RR42_RS00250 0.63 -1.1 77,006 - RR42_RS00250 0.85 +0.4 77,069 + -2.4 77,276 + -1.5 77,276 + -0.2 77,276 + -0.1 77,276 + -0.0 77,276 + +0.8 77,277 - +0.3 77,444 + -0.0 77,444 + -0.3 77,616 - RR42_RS00255 0.26 -0.8 77,739 + RR42_RS00255 0.44 -0.2 77,750 - RR42_RS00255 0.46 -0.3 77,879 + RR42_RS00255 0.64 +1.2 77,880 - RR42_RS00255 0.65 -0.7 77,880 - RR42_RS00255 0.65 +0.0 78,008 + RR42_RS00255 0.83 +0.3
Or see this region's nucleotide sequence