Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_913 and Pf1N1B4_914 are separated by 65 nucleotides Pf1N1B4_914 and Pf1N1B4_915 are separated by 11 nucleotides Pf1N1B4_915 and Pf1N1B4_916 are separated by 10 nucleotides
Pf1N1B4_913 - FIG00958224: hypothetical protein, at 1,038,664 to 1,039,152
_913
Pf1N1B4_914 - ABC-type polar amino acid transport system, ATPase component, at 1,039,218 to 1,039,982
_914
Pf1N1B4_915 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 1,039,994 to 1,041,091
_915
Pf1N1B4_916 - Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4), at 1,041,102 to 1,042,229
_916
Position (kb)
1039
1040
1041
1042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1039.001 kb on + strand, within Pf1N1B4_913 at 1039.001 kb on + strand, within Pf1N1B4_913 at 1039.001 kb on + strand, within Pf1N1B4_913 at 1039.001 kb on + strand, within Pf1N1B4_913 at 1039.001 kb on + strand, within Pf1N1B4_913 at 1039.002 kb on - strand, within Pf1N1B4_913 at 1039.029 kb on - strand, within Pf1N1B4_913 at 1039.029 kb on - strand, within Pf1N1B4_913 at 1039.029 kb on - strand, within Pf1N1B4_913 at 1039.037 kb on + strand, within Pf1N1B4_913 at 1039.037 kb on + strand, within Pf1N1B4_913 at 1039.037 kb on + strand at 1039.054 kb on + strand, within Pf1N1B4_913 at 1039.054 kb on + strand, within Pf1N1B4_913 at 1039.055 kb on - strand, within Pf1N1B4_913 at 1039.055 kb on - strand, within Pf1N1B4_913 at 1039.106 kb on + strand at 1039.106 kb on + strand at 1039.106 kb on + strand at 1039.106 kb on + strand at 1039.106 kb on + strand at 1039.106 kb on + strand at 1039.106 kb on + strand at 1039.107 kb on - strand at 1039.107 kb on - strand at 1039.107 kb on - strand at 1039.107 kb on - strand at 1039.107 kb on - strand at 1039.173 kb on + strand at 1039.197 kb on + strand at 1039.547 kb on - strand, within Pf1N1B4_914 at 1039.547 kb on - strand, within Pf1N1B4_914 at 1039.550 kb on + strand, within Pf1N1B4_914 at 1039.550 kb on + strand, within Pf1N1B4_914 at 1039.550 kb on + strand, within Pf1N1B4_914 at 1039.550 kb on + strand, within Pf1N1B4_914 at 1039.551 kb on - strand, within Pf1N1B4_914 at 1039.551 kb on - strand, within Pf1N1B4_914 at 1039.551 kb on - strand, within Pf1N1B4_914 at 1039.573 kb on - strand, within Pf1N1B4_914 at 1039.585 kb on + strand at 1039.585 kb on + strand, within Pf1N1B4_914 at 1039.585 kb on + strand, within Pf1N1B4_914 at 1039.586 kb on - strand, within Pf1N1B4_914 at 1039.630 kb on - strand, within Pf1N1B4_914 at 1039.941 kb on - strand at 1039.983 kb on - strand at 1039.983 kb on - strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.987 kb on + strand at 1039.988 kb on - strand at 1039.988 kb on - strand at 1039.988 kb on - strand at 1039.988 kb on - strand at 1039.988 kb on - strand at 1039.996 kb on - strand at 1039.996 kb on - strand at 1040.020 kb on + strand at 1040.020 kb on + strand at 1040.020 kb on + strand at 1040.021 kb on - strand at 1040.021 kb on - strand at 1040.021 kb on - strand at 1040.021 kb on - strand at 1040.021 kb on - strand at 1040.021 kb on - strand at 1040.051 kb on - strand at 1040.091 kb on + strand at 1040.092 kb on - strand at 1040.092 kb on - strand at 1040.092 kb on - strand at 1040.212 kb on + strand, within Pf1N1B4_915 at 1040.311 kb on - strand, within Pf1N1B4_915 at 1040.361 kb on + strand, within Pf1N1B4_915 at 1040.361 kb on + strand, within Pf1N1B4_915 at 1040.361 kb on + strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.362 kb on - strand, within Pf1N1B4_915 at 1040.682 kb on + strand, within Pf1N1B4_915 at 1040.683 kb on - strand, within Pf1N1B4_915 at 1040.697 kb on + strand, within Pf1N1B4_915 at 1040.698 kb on - strand, within Pf1N1B4_915 at 1040.722 kb on - strand, within Pf1N1B4_915 at 1040.722 kb on - strand, within Pf1N1B4_915 at 1040.795 kb on + strand, within Pf1N1B4_915 at 1040.824 kb on - strand, within Pf1N1B4_915 at 1040.829 kb on + strand, within Pf1N1B4_915 at 1040.829 kb on + strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.830 kb on - strand, within Pf1N1B4_915 at 1040.951 kb on + strand, within Pf1N1B4_915 at 1040.952 kb on - strand, within Pf1N1B4_915 at 1040.952 kb on - strand, within Pf1N1B4_915 at 1040.952 kb on - strand, within Pf1N1B4_915 at 1040.952 kb on - strand, within Pf1N1B4_915 at 1040.952 kb on - strand, within Pf1N1B4_915 at 1040.958 kb on + strand, within Pf1N1B4_915 at 1040.968 kb on - strand, within Pf1N1B4_915 at 1040.968 kb on - strand, within Pf1N1B4_915 at 1040.968 kb on - strand, within Pf1N1B4_915 at 1040.968 kb on - strand, within Pf1N1B4_915 at 1041.079 kb on + strand at 1041.079 kb on + strand at 1041.079 kb on + strand at 1041.080 kb on - strand at 1041.080 kb on - strand at 1041.133 kb on + strand at 1041.133 kb on + strand at 1041.133 kb on + strand at 1041.133 kb on + strand at 1041.134 kb on - strand at 1041.175 kb on + strand at 1041.176 kb on - strand at 1041.307 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.473 kb on - strand, within Pf1N1B4_916 at 1041.809 kb on + strand, within Pf1N1B4_916 at 1041.900 kb on + strand, within Pf1N1B4_916 at 1041.900 kb on + strand, within Pf1N1B4_916 at 1041.901 kb on - strand, within Pf1N1B4_916 at 1041.901 kb on - strand, within Pf1N1B4_916 at 1041.901 kb on - strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.063 kb on + strand, within Pf1N1B4_916 at 1042.064 kb on - strand, within Pf1N1B4_916 at 1042.064 kb on - strand, within Pf1N1B4_916
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,039,001 + Pf1N1B4_913 0.69 +1.1 1,039,001 + Pf1N1B4_913 0.69 -0.7 1,039,001 + Pf1N1B4_913 0.69 +0.7 1,039,001 + Pf1N1B4_913 0.69 -0.6 1,039,001 + Pf1N1B4_913 0.69 +0.7 1,039,002 - Pf1N1B4_913 0.69 -0.9 1,039,029 - Pf1N1B4_913 0.75 +1.1 1,039,029 - Pf1N1B4_913 0.75 -0.4 1,039,029 - Pf1N1B4_913 0.75 -0.3 1,039,037 + Pf1N1B4_913 0.76 -1.5 1,039,037 + Pf1N1B4_913 0.76 -1.7 1,039,037 + +0.1 1,039,054 + Pf1N1B4_913 0.80 -0.1 1,039,054 + Pf1N1B4_913 0.80 +0.3 1,039,055 - Pf1N1B4_913 0.80 -1.5 1,039,055 - Pf1N1B4_913 0.80 -0.2 1,039,106 + +0.1 1,039,106 + -0.1 1,039,106 + -1.4 1,039,106 + -0.2 1,039,106 + -0.6 1,039,106 + -0.5 1,039,106 + +0.7 1,039,107 - +2.1 1,039,107 - +0.8 1,039,107 - +0.1 1,039,107 - -0.3 1,039,107 - +0.7 1,039,173 + -0.2 1,039,197 + -1.5 1,039,547 - Pf1N1B4_914 0.43 +0.1 1,039,547 - Pf1N1B4_914 0.43 -0.2 1,039,550 + Pf1N1B4_914 0.43 -1.9 1,039,550 + Pf1N1B4_914 0.43 +0.7 1,039,550 + Pf1N1B4_914 0.43 -0.7 1,039,550 + Pf1N1B4_914 0.43 +0.1 1,039,551 - Pf1N1B4_914 0.44 +0.1 1,039,551 - Pf1N1B4_914 0.44 -1.0 1,039,551 - Pf1N1B4_914 0.44 -2.1 1,039,573 - Pf1N1B4_914 0.46 +0.7 1,039,585 + +0.1 1,039,585 + Pf1N1B4_914 0.48 -0.5 1,039,585 + Pf1N1B4_914 0.48 +0.5 1,039,586 - Pf1N1B4_914 0.48 +0.1 1,039,630 - Pf1N1B4_914 0.54 -1.2 1,039,941 - +0.1 1,039,983 - -2.2 1,039,983 - -2.2 1,039,987 + -0.9 1,039,987 + -2.4 1,039,987 + -0.3 1,039,987 + -1.2 1,039,987 + -0.7 1,039,987 + -1.1 1,039,987 + -0.9 1,039,987 + +1.7 1,039,988 - -0.3 1,039,988 - +1.7 1,039,988 - +0.1 1,039,988 - -0.9 1,039,988 - -0.5 1,039,996 - +0.7 1,039,996 - -0.2 1,040,020 + +1.1 1,040,020 + -0.9 1,040,020 + +1.8 1,040,021 - -1.4 1,040,021 - +0.1 1,040,021 - -0.0 1,040,021 - -0.9 1,040,021 - -1.1 1,040,021 - -1.2 1,040,051 - +1.1 1,040,091 + +0.1 1,040,092 - +0.1 1,040,092 - +1.1 1,040,092 - -1.5 1,040,212 + Pf1N1B4_915 0.20 -1.5 1,040,311 - Pf1N1B4_915 0.29 -2.2 1,040,361 + Pf1N1B4_915 0.33 +0.1 1,040,361 + Pf1N1B4_915 0.33 -0.9 1,040,361 + Pf1N1B4_915 0.33 -0.2 1,040,362 - Pf1N1B4_915 0.34 -1.2 1,040,362 - +1.1 1,040,362 - Pf1N1B4_915 0.34 -0.5 1,040,362 - Pf1N1B4_915 0.34 -1.9 1,040,362 - Pf1N1B4_915 0.34 -0.9 1,040,362 - Pf1N1B4_915 0.34 +0.5 1,040,362 - Pf1N1B4_915 0.34 +0.1 1,040,362 - Pf1N1B4_915 0.34 +0.1 1,040,362 - Pf1N1B4_915 0.34 +2.1 1,040,682 + Pf1N1B4_915 0.63 -0.5 1,040,683 - Pf1N1B4_915 0.63 -1.5 1,040,697 + Pf1N1B4_915 0.64 -3.1 1,040,698 - Pf1N1B4_915 0.64 +1.7 1,040,722 - Pf1N1B4_915 0.66 +0.7 1,040,722 - Pf1N1B4_915 0.66 +1.1 1,040,795 + Pf1N1B4_915 0.73 -1.9 1,040,824 - Pf1N1B4_915 0.76 -1.7 1,040,829 + Pf1N1B4_915 0.76 +0.1 1,040,829 + Pf1N1B4_915 0.76 -1.1 1,040,830 - Pf1N1B4_915 0.76 +1.7 1,040,830 - Pf1N1B4_915 0.76 -1.7 1,040,830 - Pf1N1B4_915 0.76 -1.9 1,040,830 - Pf1N1B4_915 0.76 -2.1 1,040,830 - Pf1N1B4_915 0.76 -2.5 1,040,830 - Pf1N1B4_915 0.76 -1.3 1,040,830 - Pf1N1B4_915 0.76 -0.5 1,040,951 + Pf1N1B4_915 0.87 -0.9 1,040,952 - Pf1N1B4_915 0.87 -2.2 1,040,952 - Pf1N1B4_915 0.87 -0.9 1,040,952 - Pf1N1B4_915 0.87 -2.2 1,040,952 - Pf1N1B4_915 0.87 -0.7 1,040,952 - Pf1N1B4_915 0.87 +0.4 1,040,958 + Pf1N1B4_915 0.88 +2.1 1,040,968 - Pf1N1B4_915 0.89 +0.1 1,040,968 - Pf1N1B4_915 0.89 -0.9 1,040,968 - Pf1N1B4_915 0.89 -1.5 1,040,968 - Pf1N1B4_915 0.89 +0.5 1,041,079 + -1.2 1,041,079 + -1.5 1,041,079 + -0.9 1,041,080 - -0.9 1,041,080 - -0.9 1,041,133 + +0.1 1,041,133 + +0.1 1,041,133 + -0.5 1,041,133 + +0.1 1,041,134 - +0.7 1,041,175 + -0.5 1,041,176 - -0.9 1,041,307 + Pf1N1B4_916 0.18 -3.1 1,041,472 + Pf1N1B4_916 0.33 -0.9 1,041,472 + Pf1N1B4_916 0.33 -2.5 1,041,472 + Pf1N1B4_916 0.33 -1.9 1,041,472 + Pf1N1B4_916 0.33 +1.1 1,041,473 - Pf1N1B4_916 0.33 -0.6 1,041,809 + Pf1N1B4_916 0.63 -1.9 1,041,900 + Pf1N1B4_916 0.71 +0.1 1,041,900 + Pf1N1B4_916 0.71 -0.1 1,041,901 - Pf1N1B4_916 0.71 -1.1 1,041,901 - Pf1N1B4_916 0.71 -0.9 1,041,901 - Pf1N1B4_916 0.71 -1.9 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,904 + +0.1 1,041,904 + Pf1N1B4_916 0.71 -1.7 1,041,904 + Pf1N1B4_916 0.71 -0.5 1,041,904 + Pf1N1B4_916 0.71 -1.9 1,041,904 + Pf1N1B4_916 0.71 +0.5 1,041,904 + Pf1N1B4_916 0.71 -0.9 1,041,904 + Pf1N1B4_916 0.71 -2.2 1,041,904 + Pf1N1B4_916 0.71 -1.5 1,041,904 + Pf1N1B4_916 0.71 -1.2 1,041,904 + Pf1N1B4_916 0.71 +0.6 1,041,904 + Pf1N1B4_916 0.71 +0.6 1,041,904 + Pf1N1B4_916 0.71 -0.9 1,041,904 + Pf1N1B4_916 0.71 -1.7 1,041,904 + +0.1 1,041,904 + Pf1N1B4_916 0.71 -0.9 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,904 + Pf1N1B4_916 0.71 -1.9 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 +0.5 1,041,905 - Pf1N1B4_916 0.71 -0.6 1,041,905 - Pf1N1B4_916 0.71 -1.7 1,041,905 - Pf1N1B4_916 0.71 -3.1 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,041,905 - Pf1N1B4_916 0.71 -1.5 1,041,905 - Pf1N1B4_916 0.71 -2.2 1,041,905 - Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,041,905 - Pf1N1B4_916 0.71 +0.7 1,041,905 - Pf1N1B4_916 0.71 -1.5 1,041,905 - Pf1N1B4_916 0.71 -0.5 1,041,905 - Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,041,905 - Pf1N1B4_916 0.71 -1.2 1,041,905 - Pf1N1B4_916 0.71 -0.3 1,041,905 - Pf1N1B4_916 0.71 -1.5 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,042,045 + Pf1N1B4_916 0.84 -0.8 1,042,045 + Pf1N1B4_916 0.84 -1.7 1,042,045 + +0.1 1,042,045 + Pf1N1B4_916 0.84 -0.9 1,042,045 + Pf1N1B4_916 0.84 -0.9 1,042,045 + Pf1N1B4_916 0.84 -0.5 1,042,045 + Pf1N1B4_916 0.84 +1.9 1,042,045 + Pf1N1B4_916 0.84 -1.7 1,042,045 + Pf1N1B4_916 0.84 -0.9 1,042,045 + Pf1N1B4_916 0.84 -3.1 1,042,045 + Pf1N1B4_916 0.84 -2.2 1,042,045 + Pf1N1B4_916 0.84 -0.2 1,042,045 + Pf1N1B4_916 0.84 +0.1 1,042,045 + Pf1N1B4_916 0.84 +0.1 1,042,046 - Pf1N1B4_916 0.84 -1.5 1,042,046 - Pf1N1B4_916 0.84 +0.1 1,042,046 - Pf1N1B4_916 0.84 -2.2 1,042,046 - Pf1N1B4_916 0.84 +1.1 1,042,046 - Pf1N1B4_916 0.84 +0.1 1,042,046 - Pf1N1B4_916 0.84 +0.1 1,042,046 - Pf1N1B4_916 0.84 -0.3 1,042,063 + Pf1N1B4_916 0.85 -0.9 1,042,064 - Pf1N1B4_916 0.85 -0.9 1,042,064 - Pf1N1B4_916 0.85 -0.4
Or see this region's nucleotide sequence