Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_738 and Pf1N1B4_739 are separated by 25 nucleotides Pf1N1B4_739 and Pf1N1B4_740 are separated by 108 nucleotides Pf1N1B4_740 and Pf1N1B4_741 are separated by 86 nucleotides
Pf1N1B4_738 - Electron transport complex protein RnfE, at 840,749 to 841,339
_738
Pf1N1B4_739 - Endonuclease III (EC 4.2.99.18), at 841,365 to 842,003
_739
Pf1N1B4_740 - Leucyl-tRNA synthetase, at 842,112 to 842,291
_740
Pf1N1B4_741 - DNA-binding response regulator, LuxR family, at 842,378 to 843,004
_741
Position (kb) 842
843 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 841.273 kb on + strand, within Pf1N1B4_738 at 841.274 kb on - strand, within Pf1N1B4_738 at 841.281 kb on + strand at 841.288 kb on - strand at 841.440 kb on + strand, within Pf1N1B4_739 at 841.441 kb on - strand, within Pf1N1B4_739 at 841.441 kb on - strand, within Pf1N1B4_739 at 841.542 kb on - strand, within Pf1N1B4_739 at 841.587 kb on + strand, within Pf1N1B4_739 at 841.587 kb on + strand, within Pf1N1B4_739 at 841.588 kb on - strand, within Pf1N1B4_739 at 841.648 kb on + strand, within Pf1N1B4_739 at 841.649 kb on - strand, within Pf1N1B4_739 at 841.675 kb on + strand, within Pf1N1B4_739 at 841.876 kb on - strand, within Pf1N1B4_739 at 841.876 kb on - strand, within Pf1N1B4_739 at 841.918 kb on - strand, within Pf1N1B4_739 at 841.918 kb on - strand, within Pf1N1B4_739 at 841.977 kb on + strand at 841.978 kb on - strand at 841.978 kb on - strand at 841.978 kb on - strand at 841.978 kb on - strand at 841.978 kb on - strand at 842.069 kb on + strand at 842.071 kb on + strand at 842.071 kb on + strand at 842.072 kb on - strand at 842.094 kb on + strand at 842.095 kb on - strand at 842.105 kb on + strand at 842.106 kb on - strand at 842.111 kb on + strand at 842.111 kb on + strand at 842.112 kb on - strand at 842.112 kb on - strand at 842.140 kb on + strand, within Pf1N1B4_740 at 842.140 kb on + strand, within Pf1N1B4_740 at 842.140 kb on + strand, within Pf1N1B4_740 at 842.140 kb on + strand, within Pf1N1B4_740 at 842.141 kb on - strand, within Pf1N1B4_740 at 842.141 kb on - strand, within Pf1N1B4_740 at 842.287 kb on + strand at 842.287 kb on + strand at 842.287 kb on + strand at 842.287 kb on + strand at 842.288 kb on - strand at 842.296 kb on + strand at 842.296 kb on + strand at 842.296 kb on + strand at 842.296 kb on + strand at 842.297 kb on - strand at 842.324 kb on - strand at 842.327 kb on - strand at 842.327 kb on - strand at 842.327 kb on - strand at 842.382 kb on - strand at 842.459 kb on - strand, within Pf1N1B4_741 at 842.459 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.638 kb on - strand, within Pf1N1B4_741 at 842.638 kb on - strand, within Pf1N1B4_741 at 842.638 kb on - strand, within Pf1N1B4_741 at 842.643 kb on + strand, within Pf1N1B4_741 at 842.664 kb on - strand, within Pf1N1B4_741 at 842.905 kb on + strand, within Pf1N1B4_741 at 842.950 kb on + strand at 843.051 kb on - strand at 843.051 kb on - strand at 843.056 kb on - strand at 843.056 kb on - strand at 843.109 kb on + strand at 843.110 kb on - strand at 843.110 kb on - strand at 843.110 kb on - strand at 843.110 kb on - strand at 843.110 kb on - strand at 843.110 kb on - strand at 843.130 kb on + strand at 843.131 kb on - strand at 843.181 kb on + strand at 843.182 kb on - strand at 843.184 kb on + strand at 843.185 kb on - strand at 843.223 kb on - strand at 843.286 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 841,273 + Pf1N1B4_738 0.89 +1.0 841,274 - Pf1N1B4_738 0.89 -0.2 841,281 + -1.0 841,288 - -0.4 841,440 + Pf1N1B4_739 0.12 +1.6 841,441 - Pf1N1B4_739 0.12 +0.7 841,441 - Pf1N1B4_739 0.12 +0.0 841,542 - Pf1N1B4_739 0.28 +0.0 841,587 + Pf1N1B4_739 0.35 +0.0 841,587 + Pf1N1B4_739 0.35 -0.6 841,588 - Pf1N1B4_739 0.35 +0.6 841,648 + Pf1N1B4_739 0.44 -2.0 841,649 - Pf1N1B4_739 0.44 +1.6 841,675 + Pf1N1B4_739 0.49 +0.4 841,876 - Pf1N1B4_739 0.80 +1.6 841,876 - Pf1N1B4_739 0.80 +0.0 841,918 - Pf1N1B4_739 0.87 -0.3 841,918 - Pf1N1B4_739 0.87 -1.0 841,977 + +1.0 841,978 - +0.6 841,978 - -0.6 841,978 - -1.6 841,978 - -0.4 841,978 - +1.0 842,069 + -1.5 842,071 + +0.6 842,071 + +0.0 842,072 - -1.3 842,094 + -1.3 842,095 - +1.0 842,105 + +0.0 842,106 - -1.0 842,111 + -1.0 842,111 + -0.4 842,112 - +1.3 842,112 - -1.3 842,140 + Pf1N1B4_740 0.16 -1.8 842,140 + Pf1N1B4_740 0.16 -0.6 842,140 + Pf1N1B4_740 0.16 -1.2 842,140 + Pf1N1B4_740 0.16 -1.0 842,141 - Pf1N1B4_740 0.16 -0.7 842,141 - Pf1N1B4_740 0.16 -1.2 842,287 + +0.4 842,287 + -2.0 842,287 + -0.4 842,287 + +1.3 842,288 - +1.0 842,296 + +0.0 842,296 + +0.2 842,296 + +1.0 842,296 + +1.3 842,297 - -1.0 842,324 - -0.7 842,327 - +0.0 842,327 - +1.3 842,327 - +0.3 842,382 - +0.6 842,459 - Pf1N1B4_741 0.13 -1.0 842,459 - Pf1N1B4_741 0.13 -1.2 842,461 - Pf1N1B4_741 0.13 -1.8 842,461 - Pf1N1B4_741 0.13 +0.6 842,461 - Pf1N1B4_741 0.13 -0.6 842,461 - Pf1N1B4_741 0.13 -1.0 842,638 - Pf1N1B4_741 0.41 +0.3 842,638 - Pf1N1B4_741 0.41 -2.8 842,638 - Pf1N1B4_741 0.41 -1.2 842,643 + Pf1N1B4_741 0.42 +0.0 842,664 - Pf1N1B4_741 0.46 +0.0 842,905 + Pf1N1B4_741 0.84 +0.0 842,950 + +2.0 843,051 - +1.0 843,051 - -1.0 843,056 - -0.3 843,056 - -0.4 843,109 + -1.8 843,110 - +0.4 843,110 - +1.8 843,110 - -1.3 843,110 - -1.0 843,110 - +0.4 843,110 - -0.4 843,130 + -0.4 843,131 - +1.0 843,181 + -1.0 843,182 - +0.6 843,184 + +0.6 843,185 - +0.0 843,223 - +0.0 843,286 + +1.6
Or see this region's nucleotide sequence