Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_68

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotidesPf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotidesPf1N1B4_68 and Pf1N1B4_69 are separated by 293 nucleotides Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353 _66 Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186 _67 Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535 _68 Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542 _69 Position (kb) 70 71 72Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 69.207 kb on - strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.271 kb on + strand, within Pf1N1B4_66at 69.330 kb on + strandat 69.330 kb on + strandat 69.395 kb on + strandat 69.432 kb on + strandat 69.432 kb on + strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.473 kb on + strandat 69.474 kb on - strandat 69.474 kb on - strandat 69.476 kb on - strandat 69.476 kb on - strandat 69.639 kb on - strand, within Pf1N1B4_67at 69.729 kb on + strand, within Pf1N1B4_67at 69.798 kb on - strand, within Pf1N1B4_67at 70.129 kb on + strandat 70.160 kb on + strandat 70.161 kb on - strandat 70.534 kb on + strand, within Pf1N1B4_68at 70.596 kb on - strand, within Pf1N1B4_68at 71.044 kb on + strand, within Pf1N1B4_68at 71.044 kb on + strand, within Pf1N1B4_68at 71.045 kb on - strand, within Pf1N1B4_68at 71.045 kb on - strand, within Pf1N1B4_68at 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.567 kb on - strandat 71.569 kb on + strandat 71.569 kb on + strandat 71.570 kb on - strandat 71.722 kb on + strandat 71.722 kb on + strandat 71.722 kb on + strandat 71.722 kb on + strandat 71.862 kb on - strandat 71.871 kb on + strandat 71.872 kb on - strandat 72.028 kb on - strand, within Pf1N1B4_69at 72.028 kb on - strand, within Pf1N1B4_69at 72.139 kb on + strand, within Pf1N1B4_69at 72.140 kb on - strand, within Pf1N1B4_69at 72.303 kb on + strand, within Pf1N1B4_69at 72.304 kb on - strand, within Pf1N1B4_69

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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69,207 - Pf1N1B4_66 0.73 +1.0
69,207 - Pf1N1B4_66 0.73 +0.6
69,271 + Pf1N1B4_66 0.85 +1.7
69,330 + -0.3
69,330 + +0.7
69,395 + +1.7
69,432 + -3.1
69,432 + +0.1
69,433 - +0.7
69,433 - -0.3
69,433 - -0.3
69,433 - +0.1
69,473 + +1.5
69,474 - -1.3
69,474 - -2.9
69,476 - -1.3
69,476 - +0.0
69,639 - Pf1N1B4_67 0.20 -2.3
69,729 + Pf1N1B4_67 0.33 -0.3
69,798 - Pf1N1B4_67 0.43 -0.8
70,129 + -0.6
70,160 + +1.7
70,161 - -1.3
70,534 + Pf1N1B4_68 0.26 +0.2
70,596 - Pf1N1B4_68 0.30 +0.7
71,044 + Pf1N1B4_68 0.64 -0.3
71,044 + Pf1N1B4_68 0.64 +0.7
71,045 - Pf1N1B4_68 0.64 +0.3
71,045 - Pf1N1B4_68 0.64 +0.3
71,566 + +1.7
71,566 + +0.1
71,566 + -3.3
71,566 + -0.3
71,566 + -0.4
71,566 + -1.9
71,566 + +0.1
71,567 - +0.4
71,569 + -2.3
71,569 + +1.0
71,570 - -0.1
71,722 + -0.3
71,722 + -0.3
71,722 + +1.7
71,722 + -0.3
71,862 - -0.3
71,871 + -0.7
71,872 - +1.1
72,028 - Pf1N1B4_69 0.28 +2.0
72,028 - Pf1N1B4_69 0.28 +0.2
72,139 + Pf1N1B4_69 0.43 -0.3
72,140 - Pf1N1B4_69 0.44 +0.4
72,303 + Pf1N1B4_69 0.66 -1.3
72,304 - Pf1N1B4_69 0.67 +0.3

Or see this region's nucleotide sequence