Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotides Pf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotides Pf1N1B4_68 and Pf1N1B4_69 are separated by 293 nucleotides
Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353
_66
Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186
_67
Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535
_68
Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542
_69
Position (kb)
70
71
72 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.271 kb on + strand, within Pf1N1B4_66 at 69.330 kb on + strand at 69.330 kb on + strand at 69.395 kb on + strand at 69.432 kb on + strand at 69.432 kb on + strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.473 kb on + strand at 69.474 kb on - strand at 69.474 kb on - strand at 69.476 kb on - strand at 69.476 kb on - strand at 69.639 kb on - strand, within Pf1N1B4_67 at 69.729 kb on + strand, within Pf1N1B4_67 at 69.798 kb on - strand, within Pf1N1B4_67 at 70.129 kb on + strand at 70.160 kb on + strand at 70.161 kb on - strand at 70.534 kb on + strand, within Pf1N1B4_68 at 70.596 kb on - strand, within Pf1N1B4_68 at 71.044 kb on + strand, within Pf1N1B4_68 at 71.044 kb on + strand, within Pf1N1B4_68 at 71.045 kb on - strand, within Pf1N1B4_68 at 71.045 kb on - strand, within Pf1N1B4_68 at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.567 kb on - strand at 71.569 kb on + strand at 71.569 kb on + strand at 71.570 kb on - strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.862 kb on - strand at 71.871 kb on + strand at 71.872 kb on - strand at 72.028 kb on - strand, within Pf1N1B4_69 at 72.028 kb on - strand, within Pf1N1B4_69 at 72.139 kb on + strand, within Pf1N1B4_69 at 72.140 kb on - strand, within Pf1N1B4_69 at 72.303 kb on + strand, within Pf1N1B4_69 at 72.304 kb on - strand, within Pf1N1B4_69
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 69,207 - Pf1N1B4_66 0.73 +1.0 69,207 - Pf1N1B4_66 0.73 +0.6 69,271 + Pf1N1B4_66 0.85 +1.7 69,330 + -0.3 69,330 + +0.7 69,395 + +1.7 69,432 + -3.1 69,432 + +0.1 69,433 - +0.7 69,433 - -0.3 69,433 - -0.3 69,433 - +0.1 69,473 + +1.5 69,474 - -1.3 69,474 - -2.9 69,476 - -1.3 69,476 - +0.0 69,639 - Pf1N1B4_67 0.20 -2.3 69,729 + Pf1N1B4_67 0.33 -0.3 69,798 - Pf1N1B4_67 0.43 -0.8 70,129 + -0.6 70,160 + +1.7 70,161 - -1.3 70,534 + Pf1N1B4_68 0.26 +0.2 70,596 - Pf1N1B4_68 0.30 +0.7 71,044 + Pf1N1B4_68 0.64 -0.3 71,044 + Pf1N1B4_68 0.64 +0.7 71,045 - Pf1N1B4_68 0.64 +0.3 71,045 - Pf1N1B4_68 0.64 +0.3 71,566 + +1.7 71,566 + +0.1 71,566 + -3.3 71,566 + -0.3 71,566 + -0.4 71,566 + -1.9 71,566 + +0.1 71,567 - +0.4 71,569 + -2.3 71,569 + +1.0 71,570 - -0.1 71,722 + -0.3 71,722 + -0.3 71,722 + +1.7 71,722 + -0.3 71,862 - -0.3 71,871 + -0.7 71,872 - +1.1 72,028 - Pf1N1B4_69 0.28 +2.0 72,028 - Pf1N1B4_69 0.28 +0.2 72,139 + Pf1N1B4_69 0.43 -0.3 72,140 - Pf1N1B4_69 0.44 +0.4 72,303 + Pf1N1B4_69 0.66 -1.3 72,304 - Pf1N1B4_69 0.67 +0.3
Or see this region's nucleotide sequence