Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_617 and Pf1N1B4_618 are separated by 12 nucleotides Pf1N1B4_618 and Pf1N1B4_619 are separated by 216 nucleotides Pf1N1B4_619 and Pf1N1B4_620 are separated by 457 nucleotides Pf1N1B4_620 and Pf1N1B4_621 are separated by 11 nucleotides
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Pf1N1B4_618 - Acetyl-CoA carboxylase alpha subunit, at 714,686 to 715,048
_618
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Pf1N1B4_620 - Glycine cleavage system H protein, at 717,231 to 717,614
_620
Pf1N1B4_621 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 717,626 to 720,478
_621
Position (kb) 715
716
717 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.660 kb on + strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.752 kb on + strand at 714.752 kb on + strand, within Pf1N1B4_618 at 714.752 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand at 714.770 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.807 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.920 kb on + strand, within Pf1N1B4_618 at 714.975 kb on + strand, within Pf1N1B4_618 at 715.073 kb on + strand at 715.227 kb on + strand at 715.227 kb on + strand at 715.228 kb on - strand at 715.228 kb on - strand at 715.379 kb on + strand at 715.380 kb on - strand at 715.772 kb on + strand at 715.867 kb on + strand, within Pf1N1B4_619 at 716.134 kb on - strand, within Pf1N1B4_619 at 716.396 kb on + strand, within Pf1N1B4_619 at 716.397 kb on - strand, within Pf1N1B4_619 at 716.460 kb on - strand at 716.472 kb on + strand, within Pf1N1B4_619 at 716.670 kb on - strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.750 kb on + strand at 716.751 kb on - strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.843 kb on - strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.847 kb on - strand at 716.858 kb on + strand at 716.859 kb on - strand at 716.871 kb on + strand at 716.871 kb on + strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 717.072 kb on - strand at 717.221 kb on - strand at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.580 kb on - strand at 717.637 kb on + strand at 717.637 kb on + strand at 717.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 714,315 - Pf1N1B4_617 0.61 -1.2 714,315 - Pf1N1B4_617 0.61 -2.6 714,660 + -1.9 714,661 - -1.3 714,661 - -2.8 714,661 - -1.0 714,661 - +1.3 714,665 + -0.6 714,665 + -1.6 714,665 + -0.0 714,665 + -1.6 714,665 + -0.2 714,665 + -0.0 714,665 + -0.6 714,665 + -0.8 714,665 + +1.0 714,665 + -0.4 714,665 + -1.6 714,665 + -3.2 714,665 + +2.3 714,665 + +0.2 714,665 + +2.3 714,665 + -1.2 714,665 + -1.0 714,666 - -1.3 714,666 - +1.0 714,666 - -0.0 714,666 - -0.0 714,666 - -1.0 714,666 - -0.8 714,666 - -1.7 714,666 - +0.6 714,666 - -1.0 714,671 + -0.0 714,671 + -1.6 714,671 + -0.0 714,672 - -0.0 714,672 - +0.7 714,672 - -1.0 714,672 - -0.0 714,752 + -1.0 714,752 + Pf1N1B4_618 0.18 -0.0 714,752 + -1.0 714,770 + Pf1N1B4_618 0.23 +0.4 714,770 + Pf1N1B4_618 0.23 -1.4 714,770 + Pf1N1B4_618 0.23 +0.6 714,770 + +1.0 714,770 + +0.6 714,770 + Pf1N1B4_618 0.23 -0.3 714,771 - Pf1N1B4_618 0.23 +0.1 714,771 - -0.0 714,771 - Pf1N1B4_618 0.23 -2.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,807 + Pf1N1B4_618 0.33 -0.4 714,881 + Pf1N1B4_618 0.54 -0.4 714,881 + Pf1N1B4_618 0.54 -2.6 714,881 + Pf1N1B4_618 0.54 -1.0 714,881 + Pf1N1B4_618 0.54 -2.0 714,881 + Pf1N1B4_618 0.54 -0.2 714,920 + Pf1N1B4_618 0.64 +0.4 714,975 + Pf1N1B4_618 0.80 +0.6 715,073 + +0.4 715,227 + -2.6 715,227 + -1.6 715,228 - +0.3 715,228 - +0.7 715,379 + -0.0 715,380 - -0.6 715,772 + +1.0 715,867 + Pf1N1B4_619 0.40 -1.0 716,134 - Pf1N1B4_619 0.58 +0.2 716,396 + Pf1N1B4_619 0.75 -1.3 716,397 - Pf1N1B4_619 0.75 -2.3 716,460 - -0.0 716,472 + Pf1N1B4_619 0.80 -1.3 716,670 - -0.4 716,746 + +0.4 716,746 + -1.0 716,746 + +0.4 716,747 - +0.1 716,747 - +0.2 716,747 - -2.0 716,750 + -0.0 716,751 - -0.8 716,757 + -1.0 716,757 + -0.0 716,757 + -0.0 716,758 - -0.6 716,758 - -0.0 716,758 - +0.6 716,761 + +3.1 716,761 + +0.3 716,761 + -0.0 716,762 - -0.0 716,762 - -1.3 716,762 - -0.2 716,762 - +0.6 716,762 - -0.3 716,762 - -0.3 716,843 - -0.0 716,846 + -1.6 716,846 + +0.6 716,846 + -1.0 716,847 - -0.0 716,858 + -0.5 716,859 - +0.7 716,871 + -1.0 716,871 + -0.5 716,872 - +0.3 716,872 - -0.0 716,872 - -2.6 716,872 - -0.8 716,872 - -1.0 717,072 - -0.6 717,221 - +1.0 717,426 + Pf1N1B4_620 0.51 +1.0 717,426 + Pf1N1B4_620 0.51 +0.2 717,426 + Pf1N1B4_620 0.51 +1.0 717,426 + Pf1N1B4_620 0.51 -2.3 717,427 - Pf1N1B4_620 0.51 -0.4 717,427 - Pf1N1B4_620 0.51 -0.3 717,427 - Pf1N1B4_620 0.51 -2.0 717,427 - +1.0 717,427 - Pf1N1B4_620 0.51 +0.8 717,427 - Pf1N1B4_620 0.51 +0.6 717,427 - Pf1N1B4_620 0.51 -0.4 717,427 - -0.0 717,580 - -1.0 717,637 + +1.6 717,637 + -0.6 717,638 - +0.6
Or see this region's nucleotide sequence