Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_616 and Pf1N1B4_617 are separated by 191 nucleotides Pf1N1B4_617 and Pf1N1B4_618 are separated by 12 nucleotides Pf1N1B4_618 and Pf1N1B4_619 are separated by 216 nucleotides
Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552
_616
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Pf1N1B4_618 - Acetyl-CoA carboxylase alpha subunit, at 714,686 to 715,048
_618
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Position (kb) 713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 713.550 kb on + strand at 713.575 kb on + strand at 713.576 kb on - strand at 713.744 kb on - strand at 713.933 kb on + strand, within Pf1N1B4_617 at 713.996 kb on + strand at 714.126 kb on - strand, within Pf1N1B4_617 at 714.126 kb on - strand at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.660 kb on + strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.752 kb on + strand at 714.752 kb on + strand, within Pf1N1B4_618 at 714.752 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand at 714.770 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.807 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.920 kb on + strand, within Pf1N1B4_618 at 714.975 kb on + strand, within Pf1N1B4_618 at 715.073 kb on + strand at 715.227 kb on + strand at 715.227 kb on + strand at 715.228 kb on - strand at 715.228 kb on - strand at 715.379 kb on + strand at 715.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 712,770 + Pf1N1B4_616 0.23 -0.0 712,770 + Pf1N1B4_616 0.23 +0.3 712,771 - Pf1N1B4_616 0.23 -1.1 712,771 - Pf1N1B4_616 0.23 +1.6 713,550 + -0.0 713,575 + -0.8 713,576 - -1.4 713,744 - -1.0 713,933 + Pf1N1B4_617 0.20 +0.6 713,996 + -0.0 714,126 - Pf1N1B4_617 0.41 -0.0 714,126 - -1.0 714,315 - Pf1N1B4_617 0.61 -1.2 714,315 - Pf1N1B4_617 0.61 -2.6 714,660 + -1.9 714,661 - -1.3 714,661 - -2.8 714,661 - -1.0 714,661 - +1.3 714,665 + -0.6 714,665 + -1.6 714,665 + -0.0 714,665 + -1.6 714,665 + -0.2 714,665 + -0.0 714,665 + -0.6 714,665 + -0.8 714,665 + +1.0 714,665 + -0.4 714,665 + -1.6 714,665 + -3.2 714,665 + +2.3 714,665 + +0.2 714,665 + +2.3 714,665 + -1.2 714,665 + -1.0 714,666 - -1.3 714,666 - +1.0 714,666 - -0.0 714,666 - -0.0 714,666 - -1.0 714,666 - -0.8 714,666 - -1.7 714,666 - +0.6 714,666 - -1.0 714,671 + -0.0 714,671 + -1.6 714,671 + -0.0 714,672 - -0.0 714,672 - +0.7 714,672 - -1.0 714,672 - -0.0 714,752 + -1.0 714,752 + Pf1N1B4_618 0.18 -0.0 714,752 + -1.0 714,770 + Pf1N1B4_618 0.23 +0.4 714,770 + Pf1N1B4_618 0.23 -1.4 714,770 + Pf1N1B4_618 0.23 +0.6 714,770 + +1.0 714,770 + +0.6 714,770 + Pf1N1B4_618 0.23 -0.3 714,771 - Pf1N1B4_618 0.23 +0.1 714,771 - -0.0 714,771 - Pf1N1B4_618 0.23 -2.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,807 + Pf1N1B4_618 0.33 -0.4 714,881 + Pf1N1B4_618 0.54 -0.4 714,881 + Pf1N1B4_618 0.54 -2.6 714,881 + Pf1N1B4_618 0.54 -1.0 714,881 + Pf1N1B4_618 0.54 -2.0 714,881 + Pf1N1B4_618 0.54 -0.2 714,920 + Pf1N1B4_618 0.64 +0.4 714,975 + Pf1N1B4_618 0.80 +0.6 715,073 + +0.4 715,227 + -2.6 715,227 + -1.6 715,228 - +0.3 715,228 - +0.7 715,379 + -0.0 715,380 - -0.6
Or see this region's nucleotide sequence