Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5949

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5948 and Pf1N1B4_5949 are separated by 76 nucleotidesPf1N1B4_5949 and Pf1N1B4_5950 overlap by 4 nucleotidesPf1N1B4_5950 and Pf1N1B4_5951 are separated by 157 nucleotides Pf1N1B4_5948 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 397,793 to 398,626 _5948 Pf1N1B4_5949 - Phosphomannomutase (EC 5.4.2.8), at 398,703 to 399,134 _5949 Pf1N1B4_5950 - Transcriptional activator RfaH, at 399,131 to 399,637 _5950 Pf1N1B4_5951 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 399,795 to 400,631 _5951 Position (kb) 398 399 400Strain fitness (log2 ratio) -2 -1 0 1 2at 397.774 kb on + strandat 397.888 kb on + strand, within Pf1N1B4_5948at 397.888 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strandat 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 398.101 kb on - strand, within Pf1N1B4_5948at 398.214 kb on + strand, within Pf1N1B4_5948at 398.238 kb on - strand, within Pf1N1B4_5948at 398.528 kb on + strand, within Pf1N1B4_5948at 398.528 kb on + strandat 398.528 kb on + strand, within Pf1N1B4_5948at 398.529 kb on - strand, within Pf1N1B4_5948at 398.573 kb on + strandat 398.573 kb on + strandat 398.574 kb on - strandat 398.574 kb on - strandat 398.574 kb on - strandat 398.679 kb on + strandat 398.679 kb on + strandat 398.682 kb on + strandat 398.702 kb on + strandat 398.762 kb on + strand, within Pf1N1B4_5949at 398.763 kb on - strand, within Pf1N1B4_5949at 398.778 kb on + strand, within Pf1N1B4_5949at 399.635 kb on + strandat 399.701 kb on - strandat 399.796 kb on + strandat 399.896 kb on - strand, within Pf1N1B4_5951at 399.899 kb on + strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 400.013 kb on - strand, within Pf1N1B4_5951at 400.016 kb on + strand, within Pf1N1B4_5951at 400.016 kb on + strand, within Pf1N1B4_5951at 400.016 kb on + strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.019 kb on - strand, within Pf1N1B4_5951at 400.019 kb on - strand, within Pf1N1B4_5951at 400.028 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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397,774 + -0.4
397,888 + Pf1N1B4_5948 0.11 -0.2
397,888 + Pf1N1B4_5948 0.11 -0.1
397,942 + Pf1N1B4_5948 0.18 -0.4
397,942 + Pf1N1B4_5948 0.18 +0.0
397,942 + Pf1N1B4_5948 0.18 +0.8
397,942 + -0.4
397,942 + Pf1N1B4_5948 0.18 -0.4
397,942 + Pf1N1B4_5948 0.18 +0.1
397,943 - Pf1N1B4_5948 0.18 +1.2
397,943 - Pf1N1B4_5948 0.18 -0.2
397,943 - Pf1N1B4_5948 0.18 -1.7
397,943 - Pf1N1B4_5948 0.18 -0.4
398,101 - Pf1N1B4_5948 0.37 -0.8
398,214 + Pf1N1B4_5948 0.50 -0.7
398,238 - Pf1N1B4_5948 0.53 +0.6
398,528 + Pf1N1B4_5948 0.88 +1.9
398,528 + -0.4
398,528 + Pf1N1B4_5948 0.88 -0.4
398,529 - Pf1N1B4_5948 0.88 -0.8
398,573 + +0.0
398,573 + +0.3
398,574 - +0.6
398,574 - -0.3
398,574 - +0.8
398,679 + +0.0
398,679 + -0.4
398,682 + +0.1
398,702 + -0.3
398,762 + Pf1N1B4_5949 0.14 -1.0
398,763 - Pf1N1B4_5949 0.14 -2.4
398,778 + Pf1N1B4_5949 0.17 +0.0
399,635 + -0.7
399,701 - -0.8
399,796 + -0.4
399,896 - Pf1N1B4_5951 0.12 +0.9
399,899 + Pf1N1B4_5951 0.12 -1.4
399,900 - Pf1N1B4_5951 0.13 +0.8
399,900 - Pf1N1B4_5951 0.13 +0.8
399,900 - Pf1N1B4_5951 0.13 -0.6
399,900 - Pf1N1B4_5951 0.13 +0.8
399,900 - Pf1N1B4_5951 0.13 -0.7
399,900 - Pf1N1B4_5951 0.13 -0.4
399,900 - Pf1N1B4_5951 0.13 -0.1
400,013 - Pf1N1B4_5951 0.26 +0.4
400,016 + Pf1N1B4_5951 0.26 -0.1
400,016 + Pf1N1B4_5951 0.26 +0.6
400,016 + Pf1N1B4_5951 0.26 +1.2
400,017 - Pf1N1B4_5951 0.27 -1.0
400,017 - Pf1N1B4_5951 0.27 +1.2
400,017 - Pf1N1B4_5951 0.27 +1.2
400,017 - Pf1N1B4_5951 0.27 -0.9
400,017 - Pf1N1B4_5951 0.27 -2.2
400,017 - Pf1N1B4_5951 0.27 -1.9
400,019 - Pf1N1B4_5951 0.27 +0.0
400,019 - Pf1N1B4_5951 0.27 -0.2
400,028 - -0.4

Or see this region's nucleotide sequence