Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_589 and Pf1N1B4_590 are separated by 219 nucleotides Pf1N1B4_590 and Pf1N1B4_591 are separated by 108 nucleotides
Pf1N1B4_589 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 679,222 to 680,691
_589
Pf1N1B4_590 - Phosphogluconate repressor HexR, RpiR family, at 680,911 to 681,780
_590
Pf1N1B4_591 - Aldose 1-epimerase, at 681,889 to 682,758
_591
Position (kb) 680
681
682 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 680.726 kb on - strand at 680.869 kb on + strand at 680.881 kb on + strand at 680.882 kb on - strand at 680.882 kb on - strand at 680.882 kb on - strand at 680.882 kb on - strand at 680.898 kb on + strand at 680.899 kb on - strand at 681.160 kb on + strand, within Pf1N1B4_590 at 681.160 kb on + strand, within Pf1N1B4_590 at 681.160 kb on + strand at 681.161 kb on - strand, within Pf1N1B4_590 at 681.161 kb on - strand, within Pf1N1B4_590 at 681.161 kb on - strand, within Pf1N1B4_590 at 681.202 kb on + strand, within Pf1N1B4_590 at 681.203 kb on - strand, within Pf1N1B4_590 at 681.203 kb on - strand, within Pf1N1B4_590 at 681.204 kb on + strand, within Pf1N1B4_590 at 681.618 kb on + strand, within Pf1N1B4_590 at 681.618 kb on + strand, within Pf1N1B4_590 at 681.619 kb on - strand, within Pf1N1B4_590 at 681.619 kb on - strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.624 kb on + strand, within Pf1N1B4_590 at 681.624 kb on + strand, within Pf1N1B4_590 at 681.624 kb on + strand, within Pf1N1B4_590 at 681.625 kb on - strand, within Pf1N1B4_590 at 681.774 kb on + strand at 681.774 kb on + strand at 681.774 kb on + strand at 681.774 kb on + strand at 681.775 kb on - strand at 681.778 kb on + strand at 681.779 kb on - strand at 682.007 kb on - strand, within Pf1N1B4_591 at 682.007 kb on - strand at 682.269 kb on + strand, within Pf1N1B4_591 at 682.269 kb on + strand, within Pf1N1B4_591 at 682.396 kb on + strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 680,726 - -0.8 680,869 + -1.6 680,881 + +1.0 680,882 - -1.6 680,882 - -3.2 680,882 - -1.0 680,882 - -1.4 680,898 + -1.0 680,899 - -0.0 681,160 + Pf1N1B4_590 0.29 -1.6 681,160 + Pf1N1B4_590 0.29 -1.9 681,160 + -0.0 681,161 - Pf1N1B4_590 0.29 -1.3 681,161 - Pf1N1B4_590 0.29 -2.4 681,161 - Pf1N1B4_590 0.29 -1.4 681,202 + Pf1N1B4_590 0.33 -0.5 681,203 - Pf1N1B4_590 0.34 +1.5 681,203 - Pf1N1B4_590 0.34 -0.5 681,204 + Pf1N1B4_590 0.34 -1.0 681,618 + Pf1N1B4_590 0.81 -1.4 681,618 + Pf1N1B4_590 0.81 -3.0 681,619 - Pf1N1B4_590 0.81 -0.0 681,619 - Pf1N1B4_590 0.81 +0.5 681,622 + Pf1N1B4_590 0.82 -1.4 681,622 + Pf1N1B4_590 0.82 -1.5 681,622 + Pf1N1B4_590 0.82 -1.6 681,622 + Pf1N1B4_590 0.82 -1.9 681,622 + -0.0 681,622 + Pf1N1B4_590 0.82 +0.4 681,622 + Pf1N1B4_590 0.82 -0.6 681,622 + Pf1N1B4_590 0.82 -0.0 681,622 + Pf1N1B4_590 0.82 +0.7 681,622 + Pf1N1B4_590 0.82 -2.0 681,622 + -1.0 681,622 + Pf1N1B4_590 0.82 -0.9 681,622 + Pf1N1B4_590 0.82 +0.5 681,622 + Pf1N1B4_590 0.82 -1.9 681,622 + Pf1N1B4_590 0.82 -0.0 681,622 + Pf1N1B4_590 0.82 -2.0 681,622 + Pf1N1B4_590 0.82 -2.0 681,622 + Pf1N1B4_590 0.82 +1.0 681,622 + +1.0 681,622 + Pf1N1B4_590 0.82 +0.3 681,622 + Pf1N1B4_590 0.82 -1.6 681,622 + Pf1N1B4_590 0.82 -1.8 681,622 + Pf1N1B4_590 0.82 -1.4 681,622 + Pf1N1B4_590 0.82 -0.5 681,622 + Pf1N1B4_590 0.82 +1.5 681,622 + Pf1N1B4_590 0.82 -1.0 681,623 - Pf1N1B4_590 0.82 -1.0 681,623 - Pf1N1B4_590 0.82 -1.3 681,623 - Pf1N1B4_590 0.82 +2.3 681,623 - Pf1N1B4_590 0.82 -1.9 681,623 - Pf1N1B4_590 0.82 -2.9 681,623 - Pf1N1B4_590 0.82 -1.0 681,623 - Pf1N1B4_590 0.82 -0.0 681,623 - Pf1N1B4_590 0.82 -0.9 681,623 - Pf1N1B4_590 0.82 -1.6 681,623 - Pf1N1B4_590 0.82 -0.0 681,623 - Pf1N1B4_590 0.82 +1.0 681,623 - Pf1N1B4_590 0.82 +1.0 681,623 - Pf1N1B4_590 0.82 -1.0 681,623 - Pf1N1B4_590 0.82 -1.9 681,623 - Pf1N1B4_590 0.82 -1.0 681,623 - Pf1N1B4_590 0.82 +0.1 681,623 - Pf1N1B4_590 0.82 -0.0 681,623 - Pf1N1B4_590 0.82 -1.3 681,623 - Pf1N1B4_590 0.82 -0.6 681,623 - Pf1N1B4_590 0.82 -1.4 681,623 - Pf1N1B4_590 0.82 +1.0 681,623 - Pf1N1B4_590 0.82 -2.6 681,623 - Pf1N1B4_590 0.82 -1.4 681,623 - Pf1N1B4_590 0.82 -0.0 681,623 - Pf1N1B4_590 0.82 -0.7 681,623 - Pf1N1B4_590 0.82 -1.6 681,623 - Pf1N1B4_590 0.82 -0.7 681,623 - Pf1N1B4_590 0.82 -1.5 681,623 - Pf1N1B4_590 0.82 -2.4 681,623 - Pf1N1B4_590 0.82 -0.8 681,623 - Pf1N1B4_590 0.82 -0.0 681,623 - Pf1N1B4_590 0.82 -1.6 681,623 - Pf1N1B4_590 0.82 -1.3 681,623 - Pf1N1B4_590 0.82 -0.4 681,623 - Pf1N1B4_590 0.82 -0.6 681,623 - Pf1N1B4_590 0.82 -0.3 681,623 - Pf1N1B4_590 0.82 -1.6 681,623 - Pf1N1B4_590 0.82 -1.6 681,624 + Pf1N1B4_590 0.82 -0.6 681,624 + Pf1N1B4_590 0.82 -1.0 681,624 + Pf1N1B4_590 0.82 +2.0 681,625 - Pf1N1B4_590 0.82 -1.0 681,774 + -0.7 681,774 + -1.0 681,774 + -1.0 681,774 + +1.0 681,775 - -1.0 681,778 + -0.6 681,779 - -0.6 682,007 - Pf1N1B4_591 0.14 +0.2 682,007 - -0.0 682,269 + Pf1N1B4_591 0.44 -0.0 682,269 + Pf1N1B4_591 0.44 -0.5 682,396 + Pf1N1B4_591 0.58 -0.6 682,397 - Pf1N1B4_591 0.58 -0.8 682,397 - Pf1N1B4_591 0.58 +0.4 682,397 - Pf1N1B4_591 0.58 -0.0 682,397 - Pf1N1B4_591 0.58 -0.6 682,397 - Pf1N1B4_591 0.58 -0.0 682,742 - -1.4
Or see this region's nucleotide sequence