Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5853 and Pf1N1B4_5854 overlap by 17 nucleotides Pf1N1B4_5854 and Pf1N1B4_5855 are separated by 57 nucleotides
Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586
_5853
Pf1N1B4_5854 - FIG00960144: hypothetical protein, at 286,570 to 287,322
_5854
Pf1N1B4_5855 - Putrescine importer, at 287,380 to 288,729
_5855
Position (kb) 286
287
288 Strain fitness (log2 ratio) -2
-1
0
1
2 at 286.005 kb on + strand, within Pf1N1B4_5853 at 286.005 kb on + strand, within Pf1N1B4_5853 at 286.071 kb on + strand, within Pf1N1B4_5853 at 286.116 kb on + strand, within Pf1N1B4_5853 at 286.116 kb on + strand, within Pf1N1B4_5853 at 286.240 kb on - strand, within Pf1N1B4_5853 at 286.570 kb on - strand at 286.607 kb on - strand at 286.624 kb on - strand at 286.819 kb on + strand at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.820 kb on - strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.092 kb on + strand, within Pf1N1B4_5854 at 287.093 kb on - strand, within Pf1N1B4_5854 at 287.173 kb on + strand, within Pf1N1B4_5854 at 287.302 kb on + strand at 287.320 kb on + strand at 287.328 kb on + strand at 287.329 kb on - strand at 287.329 kb on - strand at 287.387 kb on + strand at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.537 kb on - strand, within Pf1N1B4_5855 at 287.620 kb on + strand, within Pf1N1B4_5855 at 287.620 kb on + strand, within Pf1N1B4_5855 at 287.621 kb on - strand at 287.661 kb on + strand, within Pf1N1B4_5855 at 287.690 kb on - strand, within Pf1N1B4_5855 at 287.820 kb on + strand, within Pf1N1B4_5855 at 287.821 kb on - strand, within Pf1N1B4_5855 at 287.852 kb on + strand, within Pf1N1B4_5855 at 287.891 kb on - strand, within Pf1N1B4_5855 at 287.922 kb on + strand, within Pf1N1B4_5855 at 287.950 kb on + strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 288.194 kb on - strand, within Pf1N1B4_5855
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 286,005 + Pf1N1B4_5853 0.56 -1.2 286,005 + Pf1N1B4_5853 0.56 +0.2 286,071 + Pf1N1B4_5853 0.61 +1.2 286,116 + Pf1N1B4_5853 0.65 -0.2 286,116 + Pf1N1B4_5853 0.65 +2.2 286,240 - Pf1N1B4_5853 0.74 -0.4 286,570 - +1.2 286,607 - -0.4 286,624 - +0.6 286,819 + +1.2 286,819 + Pf1N1B4_5854 0.33 +0.2 286,819 + Pf1N1B4_5854 0.33 -0.7 286,820 - Pf1N1B4_5854 0.33 -0.4 287,051 + Pf1N1B4_5854 0.64 +0.6 287,051 + Pf1N1B4_5854 0.64 -0.0 287,053 + Pf1N1B4_5854 0.64 +0.2 287,053 + Pf1N1B4_5854 0.64 -0.2 287,053 + Pf1N1B4_5854 0.64 +0.4 287,053 + Pf1N1B4_5854 0.64 +0.6 287,053 + Pf1N1B4_5854 0.64 +1.1 287,092 + Pf1N1B4_5854 0.69 +1.2 287,093 - Pf1N1B4_5854 0.69 -0.4 287,173 + Pf1N1B4_5854 0.80 +0.5 287,302 + -0.4 287,320 + +0.3 287,328 + -0.1 287,329 - +1.2 287,329 - +0.9 287,387 + -2.0 287,536 + Pf1N1B4_5855 0.12 -1.2 287,536 + Pf1N1B4_5855 0.12 -0.4 287,537 - Pf1N1B4_5855 0.12 -0.4 287,620 + Pf1N1B4_5855 0.18 -2.7 287,620 + Pf1N1B4_5855 0.18 -0.4 287,621 - +0.6 287,661 + Pf1N1B4_5855 0.21 -0.1 287,690 - Pf1N1B4_5855 0.23 -0.4 287,820 + Pf1N1B4_5855 0.33 -1.0 287,821 - Pf1N1B4_5855 0.33 +1.2 287,852 + Pf1N1B4_5855 0.35 -1.4 287,891 - Pf1N1B4_5855 0.38 -2.2 287,922 + Pf1N1B4_5855 0.40 +0.4 287,950 + Pf1N1B4_5855 0.42 +1.6 287,951 - Pf1N1B4_5855 0.42 -0.4 287,951 - Pf1N1B4_5855 0.42 -1.0 287,951 - Pf1N1B4_5855 0.42 -2.0 288,194 - Pf1N1B4_5855 0.60 +0.8
Or see this region's nucleotide sequence