Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5847

Experiment: L-Isoleucine (C)

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf1N1B4_5845 and Pf1N1B4_5846 are separated by 117 nucleotidesPf1N1B4_5846 and Pf1N1B4_5847 are separated by 101 nucleotidesPf1N1B4_5847 and Pf1N1B4_5848 are separated by 147 nucleotidesPf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides Pf1N1B4_5845 - GMP synthase - Glutamine amidotransferase domain, at 279,680 to 280,321 _5845 Pf1N1B4_5846 - Rhodanese-related sulfurtransferase, at 280,439 to 280,873 _5846 Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949 _5847 Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852 _5848 Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337 _5849 Position (kb) 280 281 282Strain fitness (log2 ratio) -2 -1 0 1at 280.369 kb on - strandat 280.369 kb on - strandat 280.369 kb on - strandat 280.403 kb on + strandat 280.404 kb on - strandat 280.612 kb on - strand, within Pf1N1B4_5846at 280.774 kb on - strand, within Pf1N1B4_5846at 280.880 kb on + strandat 280.880 kb on + strandat 280.881 kb on - strandat 280.914 kb on - strandat 280.914 kb on - strandat 281.011 kb on + strandat 281.012 kb on - strandat 281.197 kb on - strand, within Pf1N1B4_5847at 281.402 kb on + strand, within Pf1N1B4_5847at 281.402 kb on + strand, within Pf1N1B4_5847at 281.402 kb on + strand, within Pf1N1B4_5847at 281.402 kb on + strand, within Pf1N1B4_5847at 281.402 kb on + strand, within Pf1N1B4_5847at 281.403 kb on - strand, within Pf1N1B4_5847at 281.403 kb on - strand, within Pf1N1B4_5847at 281.403 kb on - strand, within Pf1N1B4_5847at 281.403 kb on - strandat 281.425 kb on + strand, within Pf1N1B4_5847at 281.425 kb on + strandat 281.425 kb on + strand, within Pf1N1B4_5847at 281.933 kb on + strandat 281.934 kb on - strandat 281.934 kb on - strandat 282.116 kb on + strandat 282.116 kb on + strandat 282.116 kb on + strandat 282.117 kb on - strandat 282.117 kb on - strandat 282.117 kb on - strandat 282.117 kb on - strandat 282.117 kb on - strandat 282.117 kb on - strandat 282.120 kb on + strandat 282.121 kb on - strandat 282.121 kb on - strandat 282.242 kb on + strand, within Pf1N1B4_5848at 282.243 kb on - strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strandat 282.393 kb on + strandat 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strandat 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strandat 282.394 kb on - strand, within Pf1N1B4_5848at 282.495 kb on + strand, within Pf1N1B4_5848at 282.496 kb on - strandat 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.504 kb on - strand, within Pf1N1B4_5848at 282.504 kb on - strand, within Pf1N1B4_5848at 282.623 kb on + strandat 282.692 kb on + strand, within Pf1N1B4_5848at 282.692 kb on + strand, within Pf1N1B4_5848at 282.693 kb on - strand, within Pf1N1B4_5848at 282.888 kb on + strandat 282.889 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
remove
280,369 - +0.2
280,369 - -0.4
280,369 - -0.4
280,403 + -1.4
280,404 - -1.4
280,612 - Pf1N1B4_5846 0.40 -2.2
280,774 - Pf1N1B4_5846 0.77 -0.4
280,880 + -0.0
280,880 + +0.6
280,881 - -2.4
280,914 - -2.4
280,914 - -0.4
281,011 + +0.8
281,012 - -0.7
281,197 - Pf1N1B4_5847 0.23 +0.6
281,402 + Pf1N1B4_5847 0.44 +0.2
281,402 + Pf1N1B4_5847 0.44 +1.2
281,402 + Pf1N1B4_5847 0.44 +0.3
281,402 + Pf1N1B4_5847 0.44 -2.0
281,402 + Pf1N1B4_5847 0.44 -0.2
281,403 - Pf1N1B4_5847 0.44 -0.2
281,403 - Pf1N1B4_5847 0.44 -0.4
281,403 - Pf1N1B4_5847 0.44 -0.2
281,403 - -0.4
281,425 + Pf1N1B4_5847 0.46 +0.8
281,425 + -0.4
281,425 + Pf1N1B4_5847 0.46 +0.5
281,933 + -2.0
281,934 - -0.4
281,934 - -0.1
282,116 + -1.4
282,116 + -0.4
282,116 + +1.6
282,117 - -0.4
282,117 - +1.6
282,117 - -2.0
282,117 - +0.2
282,117 - +0.2
282,117 - -0.2
282,120 + +0.6
282,121 - +0.6
282,121 - -0.0
282,242 + Pf1N1B4_5848 0.19 +0.3
282,243 - Pf1N1B4_5848 0.19 -0.8
282,393 + Pf1N1B4_5848 0.39 +0.3
282,393 + Pf1N1B4_5848 0.39 -0.6
282,393 + Pf1N1B4_5848 0.39 +0.6
282,393 + +0.6
282,393 + -1.0
282,393 + Pf1N1B4_5848 0.39 -2.2
282,393 + Pf1N1B4_5848 0.39 -1.3
282,394 - Pf1N1B4_5848 0.39 -0.4
282,394 - Pf1N1B4_5848 0.39 -0.4
282,394 - +1.2
282,394 - Pf1N1B4_5848 0.39 -2.4
282,394 - Pf1N1B4_5848 0.39 -0.3
282,394 - -0.4
282,394 - Pf1N1B4_5848 0.39 -0.8
282,495 + Pf1N1B4_5848 0.53 +0.3
282,496 - -0.4
282,503 + Pf1N1B4_5848 0.54 +0.1
282,503 + Pf1N1B4_5848 0.54 -0.4
282,503 + Pf1N1B4_5848 0.54 +0.1
282,503 + Pf1N1B4_5848 0.54 -1.0
282,503 + Pf1N1B4_5848 0.54 -0.8
282,504 - Pf1N1B4_5848 0.54 +0.2
282,504 - Pf1N1B4_5848 0.54 +0.3
282,623 + -0.4
282,692 + Pf1N1B4_5848 0.79 -2.1
282,692 + Pf1N1B4_5848 0.79 -2.4
282,693 - Pf1N1B4_5848 0.79 -0.9
282,888 + -0.9
282,889 - +0.3

Or see this region's nucleotide sequence