Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5803

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5802 and Pf1N1B4_5803 are separated by 226 nucleotidesPf1N1B4_5803 and Pf1N1B4_5804 are separated by 51 nucleotidesPf1N1B4_5804 and Pf1N1B4_5805 are separated by 10 nucleotides Pf1N1B4_5802 - benABC operon transcriptional activator BenR, at 236,893 to 237,744 _5802 Pf1N1B4_5803 - Catechol 1,2-dioxygenase (EC 1.13.11.1), at 237,971 to 238,900 _5803 Pf1N1B4_5804 - Muconolactone isomerase (EC 5.3.3.4), at 238,952 to 239,242 _5804 Pf1N1B4_5805 - Muconate cycloisomerase (EC 5.5.1.1), at 239,253 to 240,542 _5805 Position (kb) 237 238 239Strain fitness (log2 ratio) -2 -1 0 1 2at 237.046 kb on - strand, within Pf1N1B4_5802at 237.046 kb on - strand, within Pf1N1B4_5802at 237.046 kb on - strand, within Pf1N1B4_5802at 237.072 kb on - strand, within Pf1N1B4_5802at 237.113 kb on + strand, within Pf1N1B4_5802at 237.192 kb on - strand, within Pf1N1B4_5802at 237.192 kb on - strand, within Pf1N1B4_5802at 237.294 kb on - strandat 237.507 kb on - strand, within Pf1N1B4_5802at 237.507 kb on - strand, within Pf1N1B4_5802at 237.507 kb on - strand, within Pf1N1B4_5802at 237.507 kb on - strand, within Pf1N1B4_5802at 237.564 kb on - strand, within Pf1N1B4_5802at 237.564 kb on - strand, within Pf1N1B4_5802at 237.564 kb on - strand, within Pf1N1B4_5802at 237.564 kb on - strand, within Pf1N1B4_5802at 237.623 kb on + strand, within Pf1N1B4_5802at 237.624 kb on - strand, within Pf1N1B4_5802at 237.648 kb on + strandat 237.779 kb on + strandat 237.893 kb on + strandat 237.894 kb on - strandat 237.927 kb on + strandat 238.146 kb on + strand, within Pf1N1B4_5803at 238.147 kb on - strandat 238.167 kb on + strandat 238.167 kb on + strand, within Pf1N1B4_5803at 238.167 kb on + strand, within Pf1N1B4_5803at 238.168 kb on - strand, within Pf1N1B4_5803at 238.168 kb on - strand, within Pf1N1B4_5803at 238.413 kb on + strand, within Pf1N1B4_5803at 238.572 kb on + strand, within Pf1N1B4_5803at 238.572 kb on + strand, within Pf1N1B4_5803at 238.654 kb on - strand, within Pf1N1B4_5803at 238.654 kb on - strand, within Pf1N1B4_5803at 238.719 kb on + strand, within Pf1N1B4_5803at 238.720 kb on - strand, within Pf1N1B4_5803at 238.835 kb on - strandat 238.918 kb on + strandat 238.918 kb on + strandat 238.923 kb on - strandat 239.016 kb on + strand, within Pf1N1B4_5804at 239.085 kb on + strandat 239.085 kb on + strand, within Pf1N1B4_5804at 239.094 kb on + strand, within Pf1N1B4_5804at 239.094 kb on + strand, within Pf1N1B4_5804at 239.114 kb on + strand, within Pf1N1B4_5804

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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237,046 - Pf1N1B4_5802 0.18 -0.4
237,046 - Pf1N1B4_5802 0.18 -0.4
237,046 - Pf1N1B4_5802 0.18 -1.4
237,072 - Pf1N1B4_5802 0.21 +0.1
237,113 + Pf1N1B4_5802 0.26 +1.6
237,192 - Pf1N1B4_5802 0.35 -0.1
237,192 - Pf1N1B4_5802 0.35 +1.9
237,294 - -0.4
237,507 - Pf1N1B4_5802 0.72 +0.6
237,507 - Pf1N1B4_5802 0.72 -2.0
237,507 - Pf1N1B4_5802 0.72 -1.8
237,507 - Pf1N1B4_5802 0.72 -0.4
237,564 - Pf1N1B4_5802 0.79 -0.4
237,564 - Pf1N1B4_5802 0.79 +0.6
237,564 - Pf1N1B4_5802 0.79 -2.0
237,564 - Pf1N1B4_5802 0.79 -2.1
237,623 + Pf1N1B4_5802 0.86 +0.9
237,624 - Pf1N1B4_5802 0.86 -0.4
237,648 + +0.6
237,779 + -0.2
237,893 + +1.1
237,894 - -0.4
237,927 + -0.4
238,146 + Pf1N1B4_5803 0.19 -0.4
238,147 - -0.4
238,167 + -1.4
238,167 + Pf1N1B4_5803 0.21 +0.5
238,167 + Pf1N1B4_5803 0.21 +1.1
238,168 - Pf1N1B4_5803 0.21 +0.4
238,168 - Pf1N1B4_5803 0.21 -0.4
238,413 + Pf1N1B4_5803 0.48 -0.4
238,572 + Pf1N1B4_5803 0.65 -1.4
238,572 + Pf1N1B4_5803 0.65 +0.2
238,654 - Pf1N1B4_5803 0.73 -0.9
238,654 - Pf1N1B4_5803 0.73 -0.1
238,719 + Pf1N1B4_5803 0.80 +2.6
238,720 - Pf1N1B4_5803 0.81 +1.9
238,835 - -0.4
238,918 + -0.1
238,918 + +0.6
238,923 - +2.1
239,016 + Pf1N1B4_5804 0.22 -1.0
239,085 + -0.4
239,085 + Pf1N1B4_5804 0.46 -0.7
239,094 + Pf1N1B4_5804 0.49 -1.4
239,094 + Pf1N1B4_5804 0.49 -0.4
239,114 + Pf1N1B4_5804 0.56 +0.7

Or see this region's nucleotide sequence