Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5719 and Pf1N1B4_5720 are separated by 67 nucleotides Pf1N1B4_5720 and Pf1N1B4_5721 are separated by 205 nucleotides Pf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotides Pf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides
Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043
_5719
Pf1N1B4_5720 - hypothetical protein, at 163,111 to 163,356
_5720
Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714
_5721
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Position (kb) 163
164
165 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 162.759 kb on + strand, within Pf1N1B4_5719 at 162.760 kb on - strand, within Pf1N1B4_5719 at 162.876 kb on - strand, within Pf1N1B4_5719 at 162.957 kb on + strand, within Pf1N1B4_5719 at 162.958 kb on - strand at 163.056 kb on + strand at 163.058 kb on + strand at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.177 kb on - strand, within Pf1N1B4_5720 at 163.394 kb on - strand at 163.394 kb on - strand at 163.394 kb on - strand at 163.472 kb on + strand at 163.537 kb on - strand at 163.558 kb on + strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.568 kb on - strand at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.917 kb on - strand, within Pf1N1B4_5722 at 163.936 kb on - strand, within Pf1N1B4_5722 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 162,759 + Pf1N1B4_5719 0.58 +0.8 162,760 - Pf1N1B4_5719 0.58 -0.9 162,876 - Pf1N1B4_5719 0.75 -0.5 162,957 + Pf1N1B4_5719 0.87 -1.6 162,958 - +0.9 163,056 + -0.5 163,058 + +0.9 163,174 - Pf1N1B4_5720 0.26 -1.5 163,174 - Pf1N1B4_5720 0.26 -2.3 163,176 + Pf1N1B4_5720 0.26 -0.5 163,176 + Pf1N1B4_5720 0.26 -3.6 163,177 - Pf1N1B4_5720 0.27 -0.5 163,394 - +0.5 163,394 - -1.2 163,394 - -1.5 163,472 + +0.5 163,537 - -1.0 163,558 + -0.2 163,559 - -0.4 163,559 - +0.2 163,559 - -0.7 163,561 + +0.1 163,562 - -0.2 163,568 - -0.7 163,679 + Pf1N1B4_5721 0.76 -0.1 163,679 + Pf1N1B4_5721 0.76 -0.8 163,680 - Pf1N1B4_5721 0.77 +0.1 163,680 - Pf1N1B4_5721 0.77 -0.5 163,916 + Pf1N1B4_5722 0.54 -2.5 163,916 + Pf1N1B4_5722 0.54 -0.5 163,916 + Pf1N1B4_5722 0.54 -1.5 163,917 - Pf1N1B4_5722 0.54 -1.5 163,936 - Pf1N1B4_5722 0.59 -0.5 164,798 + Pf1N1B4_5724 0.70 -0.5 164,798 + Pf1N1B4_5724 0.70 -2.6 164,798 + Pf1N1B4_5724 0.70 -0.7 164,801 + Pf1N1B4_5724 0.71 -0.5 164,802 - Pf1N1B4_5724 0.71 -1.2
Or see this region's nucleotide sequence