Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5720

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5719 and Pf1N1B4_5720 are separated by 67 nucleotidesPf1N1B4_5720 and Pf1N1B4_5721 are separated by 205 nucleotidesPf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotidesPf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043 _5719 Pf1N1B4_5720 - hypothetical protein, at 163,111 to 163,356 _5720 Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714 _5721 Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091 _5722 Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424 _5723 Position (kb) 163 164Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 162.131 kb on - strandat 162.141 kb on + strandat 162.141 kb on + strandat 162.141 kb on + strandat 162.190 kb on + strandat 162.190 kb on + strandat 162.190 kb on + strandat 162.298 kb on + strandat 162.298 kb on + strandat 162.304 kb on + strandat 162.305 kb on - strandat 162.305 kb on - strandat 162.355 kb on + strandat 162.390 kb on + strandat 162.390 kb on + strandat 162.390 kb on + strandat 162.391 kb on - strandat 162.413 kb on + strandat 162.413 kb on + strandat 162.759 kb on + strand, within Pf1N1B4_5719at 162.760 kb on - strand, within Pf1N1B4_5719at 162.876 kb on - strand, within Pf1N1B4_5719at 162.957 kb on + strand, within Pf1N1B4_5719at 162.958 kb on - strandat 163.056 kb on + strandat 163.058 kb on + strandat 163.174 kb on - strand, within Pf1N1B4_5720at 163.174 kb on - strand, within Pf1N1B4_5720at 163.176 kb on + strand, within Pf1N1B4_5720at 163.176 kb on + strand, within Pf1N1B4_5720at 163.177 kb on - strand, within Pf1N1B4_5720at 163.394 kb on - strandat 163.394 kb on - strandat 163.394 kb on - strandat 163.472 kb on + strandat 163.537 kb on - strandat 163.558 kb on + strandat 163.559 kb on - strandat 163.559 kb on - strandat 163.559 kb on - strandat 163.561 kb on + strandat 163.562 kb on - strandat 163.568 kb on - strandat 163.679 kb on + strand, within Pf1N1B4_5721at 163.679 kb on + strand, within Pf1N1B4_5721at 163.680 kb on - strand, within Pf1N1B4_5721at 163.680 kb on - strand, within Pf1N1B4_5721at 163.916 kb on + strand, within Pf1N1B4_5722at 163.916 kb on + strand, within Pf1N1B4_5722at 163.916 kb on + strand, within Pf1N1B4_5722at 163.917 kb on - strand, within Pf1N1B4_5722at 163.936 kb on - strand, within Pf1N1B4_5722

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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162,131 - +1.0
162,141 + +0.1
162,141 + -1.5
162,141 + -0.5
162,190 + -0.9
162,190 + +0.5
162,190 + +0.7
162,298 + -0.8
162,298 + -1.5
162,304 + -1.5
162,305 - +0.0
162,305 - -0.7
162,355 + -0.5
162,390 + +0.3
162,390 + +0.5
162,390 + +1.9
162,391 - +1.9
162,413 + -0.7
162,413 + +1.9
162,759 + Pf1N1B4_5719 0.58 +0.8
162,760 - Pf1N1B4_5719 0.58 -0.9
162,876 - Pf1N1B4_5719 0.75 -0.5
162,957 + Pf1N1B4_5719 0.87 -1.6
162,958 - +0.9
163,056 + -0.5
163,058 + +0.9
163,174 - Pf1N1B4_5720 0.26 -1.5
163,174 - Pf1N1B4_5720 0.26 -2.3
163,176 + Pf1N1B4_5720 0.26 -0.5
163,176 + Pf1N1B4_5720 0.26 -3.6
163,177 - Pf1N1B4_5720 0.27 -0.5
163,394 - +0.5
163,394 - -1.2
163,394 - -1.5
163,472 + +0.5
163,537 - -1.0
163,558 + -0.2
163,559 - -0.4
163,559 - +0.2
163,559 - -0.7
163,561 + +0.1
163,562 - -0.2
163,568 - -0.7
163,679 + Pf1N1B4_5721 0.76 -0.1
163,679 + Pf1N1B4_5721 0.76 -0.8
163,680 - Pf1N1B4_5721 0.77 +0.1
163,680 - Pf1N1B4_5721 0.77 -0.5
163,916 + Pf1N1B4_5722 0.54 -2.5
163,916 + Pf1N1B4_5722 0.54 -0.5
163,916 + Pf1N1B4_5722 0.54 -1.5
163,917 - Pf1N1B4_5722 0.54 -1.5
163,936 - Pf1N1B4_5722 0.59 -0.5

Or see this region's nucleotide sequence