Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5683 and Pf1N1B4_5684 are separated by 209 nucleotides Pf1N1B4_5684 and Pf1N1B4_5685 are separated by 7 nucleotides Pf1N1B4_5685 and Pf1N1B4_5686 are separated by 2 nucleotides
Pf1N1B4_5683 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 122,614 to 123,501
_5683
Pf1N1B4_5684 - Glyceraldehyde-3-phosphate dehydrogenase, putative, at 123,711 to 125,336
_5684
Pf1N1B4_5685 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,344 to 125,835
_5685
Pf1N1B4_5686 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,838 to 126,239
_5686
Position (kb)
123
124
125
126 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 123.362 kb on - strand, within Pf1N1B4_5683 at 123.444 kb on + strand at 123.444 kb on + strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.494 kb on + strand at 123.495 kb on - strand at 123.547 kb on + strand at 123.666 kb on - strand at 123.771 kb on + strand at 123.772 kb on - strand at 123.804 kb on + strand at 123.805 kb on - strand at 123.867 kb on + strand at 123.868 kb on - strand at 124.269 kb on + strand, within Pf1N1B4_5684 at 124.424 kb on + strand, within Pf1N1B4_5684 at 124.425 kb on - strand, within Pf1N1B4_5684 at 124.428 kb on + strand, within Pf1N1B4_5684 at 124.429 kb on - strand, within Pf1N1B4_5684 at 124.429 kb on - strand, within Pf1N1B4_5684 at 125.016 kb on + strand, within Pf1N1B4_5684 at 125.017 kb on - strand, within Pf1N1B4_5684 at 125.158 kb on - strand, within Pf1N1B4_5684 at 125.326 kb on - strand at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.559 kb on - strand at 125.559 kb on - strand, within Pf1N1B4_5685 at 125.559 kb on - strand at 125.559 kb on - strand, within Pf1N1B4_5685 at 125.784 kb on - strand, within Pf1N1B4_5685 at 125.935 kb on + strand, within Pf1N1B4_5686 at 125.936 kb on - strand, within Pf1N1B4_5686 at 126.222 kb on + strand at 126.223 kb on - strand at 126.250 kb on + strand at 126.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 123,362 - Pf1N1B4_5683 0.84 -1.5 123,444 + +2.1 123,444 + -0.1 123,445 - -0.5 123,445 - -0.5 123,445 - -0.5 123,445 - +0.5 123,494 + -0.5 123,495 - -1.0 123,547 + -0.3 123,666 - +0.5 123,771 + +1.1 123,772 - -1.5 123,804 + +0.1 123,805 - -0.5 123,867 + +2.1 123,868 - -1.8 124,269 + Pf1N1B4_5684 0.34 -1.8 124,424 + Pf1N1B4_5684 0.44 -0.0 124,425 - Pf1N1B4_5684 0.44 -3.0 124,428 + Pf1N1B4_5684 0.44 -2.0 124,429 - Pf1N1B4_5684 0.44 +0.3 124,429 - Pf1N1B4_5684 0.44 -1.0 125,016 + Pf1N1B4_5684 0.80 -1.6 125,017 - Pf1N1B4_5684 0.80 -0.8 125,158 - Pf1N1B4_5684 0.89 -2.5 125,326 - +1.1 125,558 + Pf1N1B4_5685 0.43 -2.8 125,558 + Pf1N1B4_5685 0.43 -0.3 125,558 + Pf1N1B4_5685 0.43 -0.5 125,558 + -2.8 125,558 + Pf1N1B4_5685 0.43 -0.5 125,559 - -0.5 125,559 - Pf1N1B4_5685 0.44 -0.6 125,559 - -0.5 125,559 - Pf1N1B4_5685 0.44 +0.1 125,784 - Pf1N1B4_5685 0.89 -0.5 125,935 + Pf1N1B4_5686 0.24 -0.5 125,936 - Pf1N1B4_5686 0.24 -2.0 126,222 + +0.5 126,223 - -0.5 126,250 + +0.5 126,251 - +1.1
Or see this region's nucleotide sequence