Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5623 and Pf1N1B4_5624 are separated by 339 nucleotides Pf1N1B4_5624 and Pf1N1B4_5625 are separated by 96 nucleotides Pf1N1B4_5625 and Pf1N1B4_5626 are separated by 135 nucleotides
Pf1N1B4_5623 - hypothetical protein, at 49,605 to 50,657
_5623
Pf1N1B4_5624 - Lipase precursor (EC 3.1.1.3), at 50,997 to 51,878
_5624
Pf1N1B4_5625 - Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8), at 51,975 to 52,256
_5625
Pf1N1B4_5626 - ABC transporter, periplasmic substrate-binding protein, at 52,392 to 54,260
_5626
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.043 kb on - strand, within Pf1N1B4_5623 at 50.298 kb on + strand, within Pf1N1B4_5623 at 50.339 kb on - strand at 50.348 kb on - strand, within Pf1N1B4_5623 at 50.348 kb on - strand at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.647 kb on + strand at 50.651 kb on + strand at 50.651 kb on + strand at 50.680 kb on + strand at 50.792 kb on - strand at 50.823 kb on + strand at 50.880 kb on - strand at 50.947 kb on - strand at 51.153 kb on - strand, within Pf1N1B4_5624 at 51.585 kb on + strand, within Pf1N1B4_5624 at 51.586 kb on - strand, within Pf1N1B4_5624 at 51.682 kb on - strand, within Pf1N1B4_5624 at 51.682 kb on - strand, within Pf1N1B4_5624 at 51.837 kb on + strand at 51.838 kb on - strand at 51.838 kb on - strand at 51.916 kb on + strand at 51.966 kb on + strand at 52.323 kb on - strand at 52.438 kb on + strand at 52.441 kb on + strand at 52.441 kb on + strand at 52.441 kb on + strand at 52.442 kb on - strand at 52.488 kb on + strand at 52.604 kb on + strand, within Pf1N1B4_5626 at 52.605 kb on - strand, within Pf1N1B4_5626 at 52.660 kb on - strand, within Pf1N1B4_5626 at 52.660 kb on - strand, within Pf1N1B4_5626 at 52.660 kb on - strand, within Pf1N1B4_5626
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 50,006 - Pf1N1B4_5623 0.38 +0.0 50,006 - Pf1N1B4_5623 0.38 +1.1 50,006 - Pf1N1B4_5623 0.38 +2.3 50,043 - Pf1N1B4_5623 0.42 -1.5 50,298 + Pf1N1B4_5623 0.66 +1.5 50,339 - -0.5 50,348 - Pf1N1B4_5623 0.71 +0.6 50,348 - -1.0 50,389 - Pf1N1B4_5623 0.74 -0.8 50,389 - Pf1N1B4_5623 0.74 -2.5 50,615 - -0.5 50,615 - +0.5 50,615 - +0.1 50,615 - -2.8 50,615 - +1.1 50,615 - +1.3 50,615 - -0.5 50,615 - +0.5 50,647 + +1.1 50,651 + -0.0 50,651 + -0.5 50,680 + +0.5 50,792 - +1.1 50,823 + +0.2 50,880 - -0.8 50,947 - -1.5 51,153 - Pf1N1B4_5624 0.18 -1.9 51,585 + Pf1N1B4_5624 0.67 +0.3 51,586 - Pf1N1B4_5624 0.67 -1.3 51,682 - Pf1N1B4_5624 0.78 -0.9 51,682 - Pf1N1B4_5624 0.78 -1.5 51,837 + -0.5 51,838 - +0.5 51,838 - -1.5 51,916 + +1.5 51,966 + +1.9 52,323 - +0.1 52,438 + -2.8 52,441 + +0.5 52,441 + -0.5 52,441 + +0.3 52,442 - -0.5 52,488 + +0.3 52,604 + Pf1N1B4_5626 0.11 -0.5 52,605 - Pf1N1B4_5626 0.11 -1.0 52,660 - Pf1N1B4_5626 0.14 -0.5 52,660 - Pf1N1B4_5626 0.14 -0.0 52,660 - Pf1N1B4_5626 0.14 +0.5
Or see this region's nucleotide sequence