Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5553 and Pf1N1B4_5554 are separated by 773 nucleotides Pf1N1B4_5554 and Pf1N1B4_5555 are separated by 172 nucleotides Pf1N1B4_5555 and Pf1N1B4_5556 are separated by 203 nucleotides
Pf1N1B4_5553 - Exodeoxyribonuclease I (EC 3.1.11.1), at 324,790 to 326,202
_5553
Pf1N1B4_5554 - hypothetical protein, at 326,976 to 327,200
_5554
Pf1N1B4_5555 - hypothetical protein, at 327,373 to 327,657
_5555
Pf1N1B4_5556 - hypothetical protein, at 327,861 to 328,058
_5556
Position (kb) 326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 326.202 kb on + strand at 326.203 kb on - strand at 326.203 kb on - strand at 326.213 kb on - strand at 326.240 kb on - strand at 326.285 kb on - strand at 326.286 kb on + strand at 326.287 kb on - strand at 326.294 kb on + strand at 326.294 kb on + strand at 326.301 kb on - strand at 326.347 kb on - strand at 326.435 kb on - strand at 326.473 kb on - strand at 326.507 kb on - strand at 326.507 kb on - strand at 326.540 kb on + strand at 326.599 kb on + strand at 326.697 kb on - strand at 326.712 kb on - strand at 326.771 kb on + strand at 326.772 kb on - strand at 326.811 kb on - strand at 326.811 kb on - strand at 326.814 kb on + strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.815 kb on - strand at 326.822 kb on + strand at 326.823 kb on - strand at 326.823 kb on - strand at 326.823 kb on - strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.845 kb on + strand at 326.846 kb on - strand at 326.846 kb on - strand at 326.846 kb on - strand at 326.849 kb on + strand at 326.849 kb on + strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.850 kb on - strand at 326.992 kb on + strand at 327.041 kb on - strand, within Pf1N1B4_5554 at 327.041 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.193 kb on - strand at 327.219 kb on - strand at 327.219 kb on - strand at 327.219 kb on - strand at 327.294 kb on - strand at 327.294 kb on - strand at 327.294 kb on - strand at 327.361 kb on - strand at 327.392 kb on + strand at 327.393 kb on - strand at 327.396 kb on - strand at 327.396 kb on - strand at 327.396 kb on - strand at 327.467 kb on + strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.473 kb on + strand, within Pf1N1B4_5555 at 327.474 kb on - strand, within Pf1N1B4_5555 at 327.474 kb on - strand at 327.530 kb on - strand, within Pf1N1B4_5555 at 327.580 kb on + strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.601 kb on - strand, within Pf1N1B4_5555 at 327.601 kb on - strand, within Pf1N1B4_5555 at 327.608 kb on + strand, within Pf1N1B4_5555 at 327.609 kb on - strand, within Pf1N1B4_5555 at 327.805 kb on + strand at 327.806 kb on - strand at 327.888 kb on - strand, within Pf1N1B4_5556 at 327.952 kb on + strand, within Pf1N1B4_5556 at 327.953 kb on - strand, within Pf1N1B4_5556 at 327.953 kb on - strand at 327.953 kb on - strand, within Pf1N1B4_5556 at 327.993 kb on - strand, within Pf1N1B4_5556 at 328.144 kb on - strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.194 kb on - strand at 328.194 kb on - strand at 328.194 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 326,202 + -0.7 326,203 - -2.8 326,203 - +0.1 326,213 - +0.3 326,240 - -2.5 326,285 - +0.5 326,286 + +0.5 326,287 - +0.5 326,294 + -0.3 326,294 + -1.5 326,301 - -0.5 326,347 - +0.6 326,435 - +0.1 326,473 - +0.6 326,507 - +0.1 326,507 - -2.8 326,540 + +0.4 326,599 + -0.7 326,697 - -0.9 326,712 - -2.8 326,771 + +0.3 326,772 - +2.1 326,811 - -0.4 326,811 - +1.4 326,814 + -0.4 326,815 - -0.9 326,815 - +0.2 326,815 - +0.6 326,815 - +2.1 326,815 - +0.4 326,822 + -1.2 326,823 - -0.8 326,823 - -0.5 326,823 - +0.1 326,845 + -0.9 326,845 + -0.9 326,845 + -0.4 326,845 + -0.9 326,845 + +0.6 326,846 - -0.9 326,846 - +0.1 326,846 - -0.5 326,849 + +0.6 326,849 + +1.6 326,850 - -0.1 326,850 - -0.5 326,850 - -3.1 326,850 - +0.1 326,850 - -1.9 326,850 - +0.4 326,992 + -0.5 327,041 - Pf1N1B4_5554 0.29 +2.4 327,041 - Pf1N1B4_5554 0.29 +0.5 327,098 - Pf1N1B4_5554 0.54 -0.2 327,098 - Pf1N1B4_5554 0.54 +1.1 327,098 - Pf1N1B4_5554 0.54 +0.1 327,193 - +0.6 327,219 - +0.1 327,219 - +0.6 327,219 - -0.8 327,294 - +0.1 327,294 - +1.1 327,294 - -0.5 327,361 - +2.1 327,392 + -1.9 327,393 - -1.3 327,396 - -0.9 327,396 - +0.8 327,396 - +0.1 327,467 + Pf1N1B4_5555 0.33 +1.1 327,468 - Pf1N1B4_5555 0.33 -2.8 327,468 - Pf1N1B4_5555 0.33 +0.4 327,468 - Pf1N1B4_5555 0.33 +0.5 327,468 - Pf1N1B4_5555 0.33 +0.4 327,468 - Pf1N1B4_5555 0.33 +0.6 327,468 - Pf1N1B4_5555 0.33 -0.8 327,473 + Pf1N1B4_5555 0.35 +0.6 327,474 - Pf1N1B4_5555 0.35 -0.5 327,474 - -0.9 327,530 - Pf1N1B4_5555 0.55 +1.6 327,580 + Pf1N1B4_5555 0.73 +1.1 327,581 - Pf1N1B4_5555 0.73 -2.3 327,581 - Pf1N1B4_5555 0.73 -1.5 327,581 - Pf1N1B4_5555 0.73 -1.9 327,601 - Pf1N1B4_5555 0.80 +0.5 327,601 - Pf1N1B4_5555 0.80 +0.6 327,608 + Pf1N1B4_5555 0.82 -0.9 327,609 - Pf1N1B4_5555 0.83 -0.9 327,805 + +0.1 327,806 - -0.9 327,888 - Pf1N1B4_5556 0.14 -0.2 327,952 + Pf1N1B4_5556 0.46 +1.1 327,953 - Pf1N1B4_5556 0.46 +0.1 327,953 - +0.1 327,953 - Pf1N1B4_5556 0.46 -0.9 327,993 - Pf1N1B4_5556 0.67 +1.2 328,144 - +0.1 328,193 + -0.9 328,193 + +0.6 328,193 + -0.5 328,193 + -0.5 328,194 - +0.5 328,194 - -0.9 328,194 - -0.7
Or see this region's nucleotide sequence