Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5541 and Pf1N1B4_5542 are separated by 497 nucleotides Pf1N1B4_5542 and Pf1N1B4_5543 are separated by 370 nucleotides Pf1N1B4_5543 and Pf1N1B4_5544 are separated by 10 nucleotides
Pf1N1B4_5541 - hypothetical protein, at 307,334 to 307,822
_5541
Pf1N1B4_5542 - hypothetical protein, at 308,320 to 308,523
_5542
Pf1N1B4_5543 - hypothetical protein, at 308,894 to 309,010
_5543
Pf1N1B4_5544 - Methyl-accepting chemotaxis protein, at 309,021 to 311,015
_5544
Position (kb) 308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 307.343 kb on + strand at 307.344 kb on - strand at 307.460 kb on + strand, within Pf1N1B4_5541 at 307.472 kb on + strand, within Pf1N1B4_5541 at 307.472 kb on + strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.587 kb on + strand at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.588 kb on - strand, within Pf1N1B4_5541 at 307.588 kb on - strand, within Pf1N1B4_5541 at 307.588 kb on - strand, within Pf1N1B4_5541 at 307.656 kb on + strand, within Pf1N1B4_5541 at 307.656 kb on + strand, within Pf1N1B4_5541 at 307.657 kb on - strand, within Pf1N1B4_5541 at 307.745 kb on + strand, within Pf1N1B4_5541 at 307.746 kb on - strand, within Pf1N1B4_5541 at 307.800 kb on - strand at 307.840 kb on + strand at 307.840 kb on + strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.965 kb on - strand at 308.061 kb on - strand at 308.113 kb on + strand at 308.114 kb on - strand at 308.114 kb on - strand at 308.117 kb on + strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.122 kb on - strand at 308.122 kb on - strand at 308.129 kb on - strand at 308.129 kb on - strand at 308.152 kb on - strand at 308.152 kb on - strand at 308.152 kb on - strand at 308.252 kb on - strand at 308.366 kb on - strand, within Pf1N1B4_5542 at 308.366 kb on - strand, within Pf1N1B4_5542 at 308.449 kb on + strand, within Pf1N1B4_5542 at 308.450 kb on - strand, within Pf1N1B4_5542 at 308.521 kb on + strand at 308.522 kb on - strand at 308.522 kb on - strand at 308.574 kb on - strand at 308.574 kb on - strand at 308.637 kb on - strand at 308.695 kb on - strand at 308.695 kb on - strand at 308.702 kb on + strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.848 kb on + strand at 308.848 kb on + strand at 308.848 kb on + strand at 308.849 kb on - strand at 308.849 kb on - strand at 308.849 kb on - strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.963 kb on + strand, within Pf1N1B4_5543 at 308.964 kb on - strand, within Pf1N1B4_5543 at 308.964 kb on - strand at 308.993 kb on - strand, within Pf1N1B4_5543 at 309.004 kb on - strand at 309.004 kb on - strand at 309.004 kb on - strand at 309.004 kb on - strand at 309.036 kb on + strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.181 kb on - strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.183 kb on - strand at 309.183 kb on - strand at 309.213 kb on + strand at 309.213 kb on + strand at 309.214 kb on - strand at 309.248 kb on - strand, within Pf1N1B4_5544 at 309.330 kb on - strand, within Pf1N1B4_5544 at 309.344 kb on + strand, within Pf1N1B4_5544 at 309.359 kb on - strand at 309.359 kb on - strand at 309.398 kb on + strand, within Pf1N1B4_5544 at 309.398 kb on + strand, within Pf1N1B4_5544 at 309.410 kb on + strand, within Pf1N1B4_5544 at 309.421 kb on + strand, within Pf1N1B4_5544 at 309.421 kb on + strand, within Pf1N1B4_5544 at 309.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 307,343 + -0.9 307,344 - -0.9 307,460 + Pf1N1B4_5541 0.26 -0.5 307,472 + Pf1N1B4_5541 0.28 +0.6 307,472 + Pf1N1B4_5541 0.28 -0.5 307,473 - Pf1N1B4_5541 0.28 -0.9 307,473 - Pf1N1B4_5541 0.28 -0.9 307,473 - Pf1N1B4_5541 0.28 +0.2 307,473 - Pf1N1B4_5541 0.28 -1.3 307,473 - Pf1N1B4_5541 0.28 -1.3 307,587 + +1.1 307,587 + Pf1N1B4_5541 0.52 -1.1 307,587 + Pf1N1B4_5541 0.52 -1.9 307,587 + Pf1N1B4_5541 0.52 -0.9 307,587 + Pf1N1B4_5541 0.52 +1.1 307,587 + Pf1N1B4_5541 0.52 +0.1 307,587 + Pf1N1B4_5541 0.52 -0.5 307,587 + Pf1N1B4_5541 0.52 -0.3 307,588 - Pf1N1B4_5541 0.52 -0.7 307,588 - Pf1N1B4_5541 0.52 -0.9 307,588 - Pf1N1B4_5541 0.52 +0.1 307,656 + Pf1N1B4_5541 0.66 +0.4 307,656 + Pf1N1B4_5541 0.66 -0.9 307,657 - Pf1N1B4_5541 0.66 -1.5 307,745 + Pf1N1B4_5541 0.84 -1.5 307,746 - Pf1N1B4_5541 0.84 +2.1 307,800 - -1.5 307,840 + +1.4 307,840 + +0.1 307,841 - -0.9 307,841 - +0.1 307,841 - +0.1 307,841 - -1.4 307,841 - +0.4 307,965 - -0.5 308,061 - -0.5 308,113 + -1.9 308,114 - -2.5 308,114 - -2.3 308,117 + -2.3 308,118 - +0.6 308,118 - +0.4 308,118 - +0.1 308,118 - -1.1 308,118 - -0.2 308,118 - +1.6 308,118 - +0.1 308,122 - +1.4 308,122 - -0.9 308,129 - -2.1 308,129 - -1.5 308,152 - -0.3 308,152 - +0.1 308,152 - +1.5 308,252 - -1.5 308,366 - Pf1N1B4_5542 0.23 +0.9 308,366 - Pf1N1B4_5542 0.23 +1.1 308,449 + Pf1N1B4_5542 0.63 -1.3 308,450 - Pf1N1B4_5542 0.64 +0.1 308,521 + +0.1 308,522 - -0.5 308,522 - +0.9 308,574 - -0.2 308,574 - +0.1 308,637 - +0.1 308,695 - -1.0 308,695 - +1.1 308,702 + -1.1 308,762 - -1.5 308,762 - -0.3 308,762 - -0.3 308,762 - -1.5 308,848 + -0.4 308,848 + -0.9 308,848 + -2.3 308,849 - +1.1 308,849 - -2.8 308,849 - -0.9 308,850 + +1.1 308,850 + +0.1 308,850 + -0.9 308,850 + -0.9 308,851 - +0.3 308,851 - -1.3 308,851 - +0.3 308,851 - -1.2 308,851 - +1.6 308,851 - +2.1 308,963 + Pf1N1B4_5543 0.59 +0.1 308,964 - Pf1N1B4_5543 0.60 -0.4 308,964 - -0.9 308,993 - Pf1N1B4_5543 0.85 -1.9 309,004 - -1.2 309,004 - +0.1 309,004 - -1.8 309,004 - -0.9 309,036 + +1.9 309,037 - +0.1 309,037 - +1.1 309,037 - -0.5 309,037 - +1.1 309,037 - -0.2 309,037 - +1.1 309,180 + +1.3 309,180 + -0.3 309,180 + -0.9 309,181 - +0.4 309,182 + +0.6 309,182 + +0.6 309,182 + +0.1 309,182 + -1.5 309,183 - -0.4 309,183 - +2.1 309,213 + +0.1 309,213 + -0.8 309,214 - +0.3 309,248 - Pf1N1B4_5544 0.11 +0.1 309,330 - Pf1N1B4_5544 0.15 -0.5 309,344 + Pf1N1B4_5544 0.16 +1.3 309,359 - +0.1 309,359 - +0.1 309,398 + Pf1N1B4_5544 0.19 +0.1 309,398 + Pf1N1B4_5544 0.19 -0.3 309,410 + Pf1N1B4_5544 0.19 -0.6 309,421 + Pf1N1B4_5544 0.20 -0.2 309,421 + Pf1N1B4_5544 0.20 +2.4 309,422 - -0.9
Or see this region's nucleotide sequence