Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5495 and Pf1N1B4_5496 are separated by 68 nucleotides
Pf1N1B4_5495 - Cyclic AMP receptor protein, at 243,236 to 243,814
_5495
Pf1N1B4_5496 - hypothetical protein, at 243,883 to 244,107
_5496
Position (kb) 243
244 Strain fitness (log2 ratio) -2
-1
0
1
2 at 242.278 kb on + strand at 242.329 kb on + strand at 242.329 kb on + strand at 242.506 kb on + strand at 242.520 kb on + strand at 242.520 kb on + strand at 242.520 kb on + strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.622 kb on - strand at 242.790 kb on + strand at 242.790 kb on + strand at 242.791 kb on - strand at 242.817 kb on + strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.831 kb on + strand at 242.831 kb on + strand at 242.831 kb on + strand at 242.832 kb on - strand at 242.870 kb on + strand at 242.871 kb on - strand at 242.871 kb on - strand at 243.050 kb on - strand at 243.064 kb on + strand at 243.065 kb on - strand at 243.065 kb on - strand at 243.081 kb on + strand at 243.081 kb on + strand at 243.081 kb on + strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.155 kb on - strand at 243.292 kb on + strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.352 kb on - strand, within Pf1N1B4_5495 at 243.352 kb on - strand, within Pf1N1B4_5495 at 243.360 kb on + strand, within Pf1N1B4_5495 at 243.361 kb on - strand, within Pf1N1B4_5495 at 243.369 kb on + strand at 243.370 kb on - strand, within Pf1N1B4_5495 at 243.475 kb on - strand, within Pf1N1B4_5495 at 243.583 kb on + strand, within Pf1N1B4_5495 at 243.584 kb on - strand, within Pf1N1B4_5495 at 243.584 kb on - strand, within Pf1N1B4_5495 at 243.719 kb on - strand, within Pf1N1B4_5495 at 243.790 kb on + strand at 243.791 kb on - strand at 243.791 kb on - strand at 243.834 kb on + strand at 243.834 kb on + strand at 243.835 kb on - strand at 243.835 kb on - strand at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 244.000 kb on + strand, within Pf1N1B4_5496 at 244.001 kb on - strand, within Pf1N1B4_5496 at 244.106 kb on - strand at 244.129 kb on - strand at 244.129 kb on - strand at 244.154 kb on + strand at 244.155 kb on - strand at 244.155 kb on - strand at 244.155 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.300 kb on + strand at 244.431 kb on - strand at 244.431 kb on - strand at 244.445 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 242,278 + -1.3 242,329 + -0.5 242,329 + +1.6 242,506 + +0.7 242,520 + +1.6 242,520 + -1.9 242,520 + +1.1 242,521 - -0.3 242,521 - +0.1 242,521 - +0.5 242,521 - +0.1 242,521 - +1.1 242,521 - -0.3 242,521 - -0.4 242,622 - +0.1 242,790 + -1.9 242,790 + +0.6 242,791 - -0.9 242,817 + -0.3 242,818 - -1.0 242,818 - -0.4 242,818 - -0.5 242,818 - +0.1 242,831 + +0.5 242,831 + +1.1 242,831 + +0.1 242,832 - +2.6 242,870 + -0.1 242,871 - -1.5 242,871 - +0.1 243,050 - +1.4 243,064 + -0.9 243,065 - -1.7 243,065 - -0.3 243,081 + -0.3 243,081 + +0.1 243,081 + -0.9 243,082 - -0.9 243,082 - +1.1 243,082 - -2.2 243,082 - -0.8 243,082 - -1.3 243,082 - -0.5 243,155 - +1.1 243,292 + -2.3 243,293 - -0.7 243,293 - +0.1 243,293 - +0.8 243,296 + Pf1N1B4_5495 0.10 +0.1 243,296 + Pf1N1B4_5495 0.10 +1.1 243,296 + Pf1N1B4_5495 0.10 -0.5 243,296 + Pf1N1B4_5495 0.10 -0.9 243,296 + Pf1N1B4_5495 0.10 -1.9 243,297 - Pf1N1B4_5495 0.11 -0.9 243,297 - Pf1N1B4_5495 0.11 -1.4 243,297 - Pf1N1B4_5495 0.11 -2.5 243,351 + Pf1N1B4_5495 0.20 +0.5 243,351 + Pf1N1B4_5495 0.20 -0.5 243,351 + Pf1N1B4_5495 0.20 +0.1 243,351 + Pf1N1B4_5495 0.20 -1.9 243,352 - Pf1N1B4_5495 0.20 -1.5 243,352 - Pf1N1B4_5495 0.20 +0.6 243,360 + Pf1N1B4_5495 0.21 +0.6 243,361 - Pf1N1B4_5495 0.22 -0.2 243,369 + -1.5 243,370 - Pf1N1B4_5495 0.23 -0.9 243,475 - Pf1N1B4_5495 0.41 -0.3 243,583 + Pf1N1B4_5495 0.60 +0.3 243,584 - Pf1N1B4_5495 0.60 -0.9 243,584 - Pf1N1B4_5495 0.60 -0.9 243,719 - Pf1N1B4_5495 0.83 -1.5 243,790 + +0.4 243,791 - -1.3 243,791 - -0.9 243,834 + +1.6 243,834 + +2.4 243,835 - +0.1 243,835 - +0.1 243,922 + Pf1N1B4_5496 0.17 -2.3 243,922 + Pf1N1B4_5496 0.17 +0.5 243,922 + Pf1N1B4_5496 0.17 +0.4 243,923 - Pf1N1B4_5496 0.18 +0.7 243,923 - Pf1N1B4_5496 0.18 +1.1 243,923 - Pf1N1B4_5496 0.18 +0.6 244,000 + Pf1N1B4_5496 0.52 +1.1 244,001 - Pf1N1B4_5496 0.52 -1.4 244,106 - -2.5 244,129 - +0.1 244,129 - +0.1 244,154 + +0.9 244,155 - +0.4 244,155 - -0.2 244,155 - -0.9 244,183 - +0.1 244,183 - -0.7 244,183 - +0.1 244,183 - -0.8 244,183 - +0.5 244,183 - -2.5 244,300 + +1.6 244,431 - +0.6 244,431 - +0.1 244,445 - -0.5 244,571 - -1.5 244,571 - -1.2 244,571 - -0.7 244,571 - -0.7
Or see this region's nucleotide sequence